2005
Heterogeneity in synaptic transmission along a Drosophila larval motor axon
Guerrero G, Reiff D, Agarwal G, Ball R, Borst A, Goodman C, Isacoff E. Heterogeneity in synaptic transmission along a Drosophila larval motor axon. Nature Neuroscience 2005, 8: 1188-1196. PMID: 16116446, PMCID: PMC1402256, DOI: 10.1038/nn1526.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAxonsCalcium SignalingDiagnostic ImagingDrosophilaDrosophila ProteinsElectric StimulationEvoked PotentialsGene Expression Regulation, DevelopmentalImmunohistochemistryLarvaLuminescent ProteinsMembrane PotentialsMotor NeuronsMutagenesis, InsertionalNeuromuscular JunctionPatch-Clamp TechniquesSynaptic TransmissionConceptsNeuromuscular junctionGlutamate receptorsSynaptic transmissionAxonal branchesMotor axonsPostsynaptic terminalsIndividual boutonsBoutonsProximal connectionLarval neuromuscular junctionTransmission strengthLarval motor axonsDrosophila melanogaster larval neuromuscular junctionDistal boutonsDistal onesHigh Ca2Ca2InfluxAxonsReceptorsIn Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies
Reiff D, Ihring A, Guerrero G, Isacoff E, Joesch M, Nakai J, Borst A. In Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies. Journal Of Neuroscience 2005, 25: 4766-4778. PMID: 15888652, PMCID: PMC1464576, DOI: 10.1523/jneurosci.4900-04.2005.Peer-Reviewed Original ResearchAnimalsAnimals, Genetically ModifiedDose-Response Relationship, RadiationDrosophilaElectric StimulationFluorescence Resonance Energy TransferFluorescent DyesGene Expression RegulationGenetic EngineeringImmunohistochemistryIn Vitro TechniquesLarvaLuminescent ProteinsMicroscopy, ConfocalMolecular Probe TechniquesNeuromuscular JunctionNeuronsPresynaptic TerminalsReproducibility of ResultsTime Factors
2002
Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential
Guerrero G, Siegel M, Roska B, Loots E, Isacoff E. Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential. Biophysical Journal 2002, 83: 3607-3618. PMID: 12496128, PMCID: PMC1302436, DOI: 10.1016/s0006-3495(02)75361-7.Peer-Reviewed Original ResearchMeSH KeywordsBiosensing TechniquesFluorescence Resonance Energy TransferGreen Fluorescent ProteinsLuminescent ProteinsMembrane PotentialsMicroscopy, FluorescenceMutagenesis, Site-DirectedNeuronsOptics and PhotonicsPotassium ChannelsPotassium Channels, Voltage-GatedProtein EngineeringRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsConceptsDetector domainDual-wavelength measurementVoltage-gated ShakerWavelength measurementsOptical readoutOptical sensorsSpectral shiftElectrical signalsIntensity changesDistinct spectraFlashesReadoutSpectraFluorescence intensity changesVoltage dependenceDependenceVoltage rangeMeasurementsSensorsDistinct voltageVoltageDynamicsShift
2001
Genetically encoded optical sensors of neuronal activity and cellular function
Guerrero G, Isacoff E. Genetically encoded optical sensors of neuronal activity and cellular function. Current Opinion In Neurobiology 2001, 11: 601-607. PMID: 11595495, DOI: 10.1016/s0959-4388(00)00256-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGenetic EngineeringGreen Fluorescent ProteinsHumansIndicators and ReagentsLuminescent ProteinsNeuronsOptics and PhotonicsSignal TransductionConceptsCellular signalsFluorescent proteinSpecific cellular signalsCellular functionsIntracellular messengerSpecific mutationsStructural rearrangementsFP fluorescenceCyclic nucleotidesNeurotransmitter releaseNew insightsProteinMembrane voltageOptical sensorsNeural circuitsNucleotidesFluorescenceMessengerMutationsRearrangementNitric oxideSignalsChemical environmentNeuronal activityFusion