2005
In Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies
Reiff D, Ihring A, Guerrero G, Isacoff E, Joesch M, Nakai J, Borst A. In Vivo Performance of Genetically Encoded Indicators of Neural Activity in Flies. Journal Of Neuroscience 2005, 25: 4766-4778. PMID: 15888652, PMCID: PMC1464576, DOI: 10.1523/jneurosci.4900-04.2005.Peer-Reviewed Original ResearchAnimalsAnimals, Genetically ModifiedDose-Response Relationship, RadiationDrosophilaElectric StimulationFluorescence Resonance Energy TransferFluorescent DyesGene Expression RegulationGenetic EngineeringImmunohistochemistryIn Vitro TechniquesLarvaLuminescent ProteinsMicroscopy, ConfocalMolecular Probe TechniquesNeuromuscular JunctionNeuronsPresynaptic TerminalsReproducibility of ResultsTime Factors
2002
Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential
Guerrero G, Siegel M, Roska B, Loots E, Isacoff E. Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential. Biophysical Journal 2002, 83: 3607-3618. PMID: 12496128, PMCID: PMC1302436, DOI: 10.1016/s0006-3495(02)75361-7.Peer-Reviewed Original ResearchMeSH KeywordsBiosensing TechniquesFluorescence Resonance Energy TransferGreen Fluorescent ProteinsLuminescent ProteinsMembrane PotentialsMicroscopy, FluorescenceMutagenesis, Site-DirectedNeuronsOptics and PhotonicsPotassium ChannelsPotassium Channels, Voltage-GatedProtein EngineeringRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsConceptsDetector domainDual-wavelength measurementVoltage-gated ShakerWavelength measurementsOptical readoutOptical sensorsSpectral shiftElectrical signalsIntensity changesDistinct spectraFlashesReadoutSpectraFluorescence intensity changesVoltage dependenceDependenceVoltage rangeMeasurementsSensorsDistinct voltageVoltageDynamicsShift