2012
The Mechanistic Basis for Noncompetitive Ibogaine Inhibition of Serotonin and Dopamine Transporters*
Bulling S, Schicker K, Zhang YW, Steinkellner T, Stockner T, Gruber CW, Boehm S, Freissmuth M, Rudnick G, Sitte HH, Sandtner W. The Mechanistic Basis for Noncompetitive Ibogaine Inhibition of Serotonin and Dopamine Transporters*. Journal Of Biological Chemistry 2012, 287: 18524-18534. PMID: 22451652, PMCID: PMC3365767, DOI: 10.1074/jbc.m112.343681.Peer-Reviewed Original Research
2009
Ligand Effects on Cross-linking Support a Conformational Mechanism for Serotonin Transport*
Tao Z, Zhang YW, Agyiri A, Rudnick G. Ligand Effects on Cross-linking Support a Conformational Mechanism for Serotonin Transport*. Journal Of Biological Chemistry 2009, 284: 33807-33814. PMID: 19837674, PMCID: PMC2797150, DOI: 10.1074/jbc.m109.071977.Peer-Reviewed Original ResearchConceptsN-terminal cyanogen bromide fragmentGamma-aminobutyric acid transporterCyanogen bromide fragmentsTransmembrane 1Cysteine residuesMutagenesis strategyAcid transportersConformational mechanismSerotonin transportCysteineCorresponding positionDisulfide CrossTransportersSynaptic cleftResiduesSame molecule
2008
Involvement of serotonin transporter extracellular loop 1 in serotonin binding and transport
Mao Y, Mao Y, Mathewson L, Mao Y, Mathewson L, Gesmonde J, Sato Y, Mao Y, Mathewson L, Gesmonde J, Sato Y, Holy M, Sitte H, Rudnick G. Involvement of serotonin transporter extracellular loop 1 in serotonin binding and transport. Molecular Membrane Biology 2008, 25: 115-127. PMID: 18307099, PMCID: PMC4510095, DOI: 10.1080/09687680701633257.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBiological TransportCell MembraneHeLa CellsHumansIndicators and ReagentsKineticsLigandsMesylatesMolecular Sequence DataMutant ProteinsProtein Structure, TertiaryRatsSequence DeletionSerotoninSerotonin Plasma Membrane Transport ProteinsStructure-Activity Relationship
2007
Interaction between lysine 102 and aspartate 338 in the insect amino acid cotransporter KAAT1
Castagna M, Soragna A, Mari S, Santacroce M, Betté S, Mandela P, Rudnick G, Peres A, Sacchi V. Interaction between lysine 102 and aspartate 338 in the insect amino acid cotransporter KAAT1. American Journal Of Physiology - Cell Physiology 2007, 293: c1286-c1295. PMID: 17626242, DOI: 10.1152/ajpcell.00190.2007.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid SubstitutionAmino Acid Transport Systems, NeutralAnimalsAspartic AcidBinding SitesBiological TransportCross-Linking ReagentsCysteineDithiothreitolFemaleInsect ProteinsKineticsLepidopteraLysineModels, MolecularMolecular Sequence DataOocytesPhenanthrolinesPotassiumProtein Structure, TertiarySequence Homology, Amino AcidSodiumTryptophanXenopus laevisConceptsSingle cysteine mutantsNeutral amino acid transporterSite-directed mutagenesisAmino acid transportersTransport-associated currentNSS transportersDouble mutantXenopus laevis oocytesCysteine mutantsWild typeDependent transportLysine 102MutantsSuper familyAcid transportersPermeation pathwayAmino acidsDisulfide bondsLaevis oocytesFunctional evidenceAsp338Leucine uptakeKAAT1Spatial organizationResidues
1999
Molecular cloning, expression and characterization of a bovine serotonin transporter1The sequence reported in this paper has been deposited in the GenBank data base (accession number AF119122).1
Mortensen O, Kristensen A, Rudnick G, Wiborg O. Molecular cloning, expression and characterization of a bovine serotonin transporter1The sequence reported in this paper has been deposited in the GenBank data base (accession number AF119122).1. Brain Research 1999, 71: 120-126. PMID: 10407194, DOI: 10.1016/s0169-328x(99)00178-3.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarrier ProteinsCattleCitalopramCloning, MolecularDesipramineFemaleFluoxetineHeLa CellsHumansImipramineKineticsMembrane GlycoproteinsMembrane Transport ProteinsMolecular Sequence DataNerve Tissue ProteinsN-Methyl-3,4-methylenedioxyamphetamineOrgan SpecificityParoxetinePhylogenyPregnancyRatsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionSequence AlignmentSequence Homology, Amino AcidSerotoninSerotonin Plasma Membrane Transport ProteinsTransfectionConceptsSerotonin transporterHuman serotonin transporterExpression of SERTAdrenal glandBrain stemParathyroid glandsPharmacological profileBone marrowThyroid glandSmall intestinePharmacological targetsRT-PCR amplificationDecreased sensitivityExtracellular fluidGlandAmino acid differencesBiogenic aminesNeurotransmitter transportersDependent neurotransmitter transportersImportant antidepressantsAcid differencesDifferent tissuesAntidepressantsParoxetineDesipramine
1998
[16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry
Rudnick G. [16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry. Methods In Enzymology 1998, 296: 233-247. PMID: 9779452, DOI: 10.1016/s0076-6879(98)96018-9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNumber of ionsKinetic determinationMolecular machinesCharge movesIonsLipid bilayersNeurotransmitter moleculesMoleculesInput of energySteady-state distributionLow concentrationsChargeElectrical potentialMembraneIon gradientsStoichiometryHigh concentrationsUltimate concentrationNaConcentrationEnergyBilayersNeurotransmitter transportersNumber of NaNeurotransmitter transport
1994
Ligand binding to the serotonin transporter: equilibria, kinetics, and ion dependence.
Humphreys C, Wall S, Rudnick G. Ligand binding to the serotonin transporter: equilibria, kinetics, and ion dependence. Biochemistry 1994, 33: 9118-25. PMID: 8049215, DOI: 10.1021/bi00197a014.Peer-Reviewed Original ResearchHuman norepinephrine transporter. Biosynthetic studies using a site-directed polyclonal antibody.
Melikian H, McDonald J, Gu H, Rudnick G, Moore K, Blakely R. Human norepinephrine transporter. Biosynthetic studies using a site-directed polyclonal antibody. Journal Of Biological Chemistry 1994, 269: 12290-12297. PMID: 8163533, DOI: 10.1016/s0021-9258(17)32714-x.Peer-Reviewed Original ResearchAnimalsAntibodiesCarrier ProteinsCell LineCysteineElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayFluorescent Antibody TechniqueHeLa CellsHumansImmunoblottingKineticsMethionineMolecular WeightNorepinephrineNorepinephrine Plasma Membrane Transport ProteinsRabbitsSulfur RadioisotopesSymportersTransfectionTunicamycinStable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence.
Gu H, Wall S, Rudnick G. Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence. Journal Of Biological Chemistry 1994, 269: 7124-7130. PMID: 8125921, DOI: 10.1016/s0021-9258(17)37256-3.Peer-Reviewed Original ResearchAnimalsBinding SitesBiogenic AminesBiological TransportCarrier ProteinsCell LineDopamine Plasma Membrane Transport ProteinsHumansKineticsMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsNorepinephrine Plasma Membrane Transport ProteinsRatsRecombinant ProteinsSerotonin Plasma Membrane Transport ProteinsSymportersTransfection
1992
p-Chloroamphetamine induces serotonin release through serotonin transporters.
Rudnick G, Wall S. p-Chloroamphetamine induces serotonin release through serotonin transporters. Biochemistry 1992, 31: 6710-8. PMID: 1322169, DOI: 10.1021/bi00144a010.Peer-Reviewed Original ResearchMeSH KeywordsBinding, CompetitiveBiological TransportBlood PlateletsCarrier ProteinsCell MembraneChloridesHumansHydrogen-Ion ConcentrationImipramineKineticsLithiumLithium ChlorideMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsP-ChloroamphetamineRadioisotope Dilution TechniqueSerotoninSerotonin Plasma Membrane Transport ProteinsTritiumConceptsPlasma membrane vesiclesMembrane vesiclesChromaffin granule membrane vesiclesVesicular amine transporterATP hydrolysisBovine adrenal chromaffin granulesSerotonin transporterAdrenal chromaffin granulesPrevents accumulationAmine transportersPresence of Mg2TransportersVesiclesChromaffin granulesModel systemDelta pHHuman plateletsATPEffluxManner characteristicAccumulationTransmembraneNaCl gradientPCA's abilityP-chloroamphetamineExpression of a cloned gamma-aminobutyric acid transporter in mammalian cells.
Keynan S, Suh Y, Kanner B, Rudnick G. Expression of a cloned gamma-aminobutyric acid transporter in mammalian cells. Biochemistry 1992, 31: 1974-9. PMID: 1536839, DOI: 10.1021/bi00122a011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCarrier ProteinsChloridesCloning, MolecularDNAGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidGene ExpressionGenetic VectorsHeLa CellsHumansKineticsL CellsMembrane ProteinsMembrane Transport ProteinsMiceNerve Tissue ProteinsOrganic Anion TransportersPlasmidsPrecipitin TestsRatsSodiumTransfectionTunicamycinXenopusConceptsMammalian cellsGABA transportMouse Ltk- cellsT7 RNA polymerasePlasma membrane vesiclesL cellsApparent molecular massGABA transporterSynaptic plasma membrane vesiclesGamma-aminobutyric acid transporterPresence of tunicamycinEukaryotic expression vectorRNA polymeraseTransient expressionExpression vectorAcid transportersMembrane vesiclesStable expressionLtk- cellsFunctional expressionGAT-1Molecular massHeLa cellsTransportersTransfection
1991
Vaccinia-T7 RNA polymerase expression system: Evaluation for the expression cloning of plasma membrane transporters
Blakely R, Clark J, Rudnick G, Amara S. Vaccinia-T7 RNA polymerase expression system: Evaluation for the expression cloning of plasma membrane transporters. Analytical Biochemistry 1991, 194: 302-308. PMID: 1862934, DOI: 10.1016/0003-2697(91)90233-j.Peer-Reviewed Original ResearchSubstrate and inhibitor binding and translocation by the platelet plasma membrane serotonin transporter
Humphreys C, Beidler D, Rudnick G. Substrate and inhibitor binding and translocation by the platelet plasma membrane serotonin transporter. Biochemical Society Transactions 1991, 19: 95-98. PMID: 2037207, DOI: 10.1042/bst0190095.Peer-Reviewed Original Research
1990
Energetics of reserpine binding and occlusion by the chromaffin granule biogenic amine transporter.
Rudnick G, Steiner-Mordoch S, Fishkes H, Stern-Bach Y, Schuldiner S. Energetics of reserpine binding and occlusion by the chromaffin granule biogenic amine transporter. Biochemistry 1990, 29: 603-8. PMID: 2140052, DOI: 10.1021/bi00455a002.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAmmonium SulfateBinding SitesBiogenic AminesCarbonyl Cyanide m-Chlorophenyl HydrazoneCarrier ProteinsChloridesChromaffin GranulesChromaffin SystemDetergentsElectrochemistryHydrogen-Ion ConcentrationIonophoresKineticsLigandsMembrane PotentialsReserpineThermodynamicsThiocyanatesTime FactorsUreaConceptsBiogenic amine transportersReserpine bindingChromaffin granule membrane vesiclesAmine transportersAmine transportMembrane vesiclesDead-end complexIon translocationTransportersSubstrate moleculesBindingM ureaDelta pHTranslocationTriton XSodium dodecyl sulfateNeutral substrate moleculesTransmembraneHigh-affinity site
1989
2-Iodoimipramine, a novel ligand for the serotonin transporter.
Humphreys C, Cassel D, Rudnick G. 2-Iodoimipramine, a novel ligand for the serotonin transporter. Molecular Pharmacology 1989, 36: 620-6. PMID: 2811859.Peer-Reviewed Original Research
1988
Antidepressant binding to the porcine and human platelet serotonin transporters.
Humphreys C, Levin J, Rudnick G. Antidepressant binding to the porcine and human platelet serotonin transporters. Molecular Pharmacology 1988, 33: 657-63. PMID: 3380080.Peer-Reviewed Original Research
1987
Hydrodynamic properties of the chromaffin granule hydrogen ion pumping adenosinetriphosphatase.
Dean G, Nelson P, Agnew W, Rudnick G. Hydrodynamic properties of the chromaffin granule hydrogen ion pumping adenosinetriphosphatase. Biochemistry 1987, 26: 2301-5. PMID: 2887199, DOI: 10.1021/bi00382a034.Peer-Reviewed Original ResearchConceptsGel permeation chromatographyCm3/Permeation chromatographyHydrodynamic propertiesSedimentation equilibrium centrifugationMixed micellesHydrogen ionsMicellesStokes radiusBovine adrenal chromaffin granulesIntrinsic membrane proteinsDetergentsParticlesParticle volumePropertiesIonsEquilibrium centrifugationChromatographyMitochondrial F1F0-ATPaseAdrenal chromaffin granulesDaltonsProtein componentsMembrane proteinsProtein V
1986
Characterization of native and reconstituted hydrogen ion pumping adenosinetriphosphatase of chromaffin granules.
Dean G, Nelson P, Rudnick G. Characterization of native and reconstituted hydrogen ion pumping adenosinetriphosphatase of chromaffin granules. Biochemistry 1986, 25: 4918-25. PMID: 2876726, DOI: 10.1021/bi00365a029.Peer-Reviewed Original ResearchConceptsChromaffin granule membranesATPase activityChromaffin granulesGranule membranesATP-dependent ion pumpsInhibitor sodium azidePlatelet dense granulesReconstituted membranesATP hydrolysisATP hydrolaseNucleotide dependenceAmine transportTransport ATPasesMitochondrial ATPaseAdrenal chromaffin granulesInhibitor sensitivityGranule acidificationIon pumpsSerotonin transportATPasesATPaseMonodisperse formATPOctyl glucosideNBD-Cl
1984
Osmotic properties and internal pH of isolated rat parotid secretory granules.
Arvan P, Rudnick G, Castle J. Osmotic properties and internal pH of isolated rat parotid secretory granules. Journal Of Biological Chemistry 1984, 259: 13567-13572. PMID: 6490664, DOI: 10.1016/s0021-9258(18)90730-1.Peer-Reviewed Original ResearchSpiperone: evidence for uptake into secretory granules.
Dannies P, Rudnick M, Fishkes H, Rudnick G. Spiperone: evidence for uptake into secretory granules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1867-1870. PMID: 6584920, PMCID: PMC345023, DOI: 10.1073/pnas.81.6.1867.Peer-Reviewed Original ResearchConceptsSerotonin receptorsSecretory granulesRat pituitary tumor cellsMicroM reserpinePlatelet dense granulesPituitary tumor cellsDopamine antagonistsIntracellular storage organellesSpiperoneTumor cellsSerotonin transportDense granulesBiogenic amine transportReceptorsCell strainsDopamineEquimolar concentrationsSpecific ligandsAmine transportStorage organellesPlasma membrane transport systemsReserpineAntagonistMinNeurons