2018
Structural elements required for coupling ion and substrate transport in the neurotransmitter transporter homolog LeuT
Zhang YW, Tavoulari S, Sinning S, Aleksandrova AA, Forrest LR, Rudnick G. Structural elements required for coupling ion and substrate transport in the neurotransmitter transporter homolog LeuT. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: e8854-e8862. PMID: 30181291, PMCID: PMC6156673, DOI: 10.1073/pnas.1716870115.Peer-Reviewed Original ResearchConceptsTransporter domainConformational changesOpen stateSodium symporter familyIon-substrate couplingTransmembrane ion gradientsSymporter familyNSS transportersSubstrate bindingLeuTIntracellular substratesCysteine accessibilitySubstrate transportAccessibility of substrateTyrosine residuesConformational responseNa2 siteUncoupled movementIon gradientsExtracellular pathwaysMechanistic componentsTransportersProteinTransport of ionsBinding
2013
How do transporters couple solute movements?
Rudnick G. How do transporters couple solute movements? Molecular Membrane Biology 2013, 30: 355-359. PMID: 24147977, PMCID: PMC4077868, DOI: 10.3109/09687688.2013.842658.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2010
Reconstructing a Chloride-binding Site in a Bacterial Neurotransmitter Transporter Homologue*
Tavoulari S, Rizwan AN, Forrest LR, Rudnick G. Reconstructing a Chloride-binding Site in a Bacterial Neurotransmitter Transporter Homologue*. Journal Of Biological Chemistry 2010, 286: 2834-2842. PMID: 21115480, PMCID: PMC3024779, DOI: 10.1074/jbc.m110.186064.Peer-Reviewed Original ResearchConceptsChloride-binding siteConformational changesAdjacent binding sitesSingle point mutationProkaryotic homologuesSubstrate translocationIon-binding sitesTransporter homologueTransport proteinsNeurotransmitter transportersNeurotransmitter transportPoint mutationsBinding sitesHomologuesProteinMutationsCl(-) bindsDirect evidenceTherapeutic drugsSitesDependent formTranslocationTransportersBindsResidues
2002
Chemical Modification Strategies for Structure‐Function Studies
Rudnick G. Chemical Modification Strategies for Structure‐Function Studies. 2002, 125-141. DOI: 10.1002/0471434043.ch8.ChaptersTransmembrane domainConformational changesPolytopic membrane proteinsStructure-function studiesMembrane proteinsTransporter proteinsReactive residuesLipid bilayersProteinTransportersExternal faceExternal loopChemical modification strategiesDomainChemical modificationSubstrateCysteineResiduesSitesMembraneModificationBilayers
1998
Bioenergetics of Neurotransmitter Transport
Rudnick G. Bioenergetics of Neurotransmitter Transport. Journal Of Bioenergetics And Biomembranes 1998, 30: 173-185. PMID: 9672239, DOI: 10.1023/a:1020573325823.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsSynaptic vesicle membranePlasma membrane transportersRecycling of neurotransmittersGene familyVesicle lumenPlasma membraneMembrane transportersNeurotransmitter transportersVesicle membraneNeurotransmitter transportVesicular transportersAmino acidsTransportersCoupling stoichiometryDownhill movementCytoplasmEssential componentUphill transportFamilyMembraneEffluxBioenergeticsImportant drugsProteinInflux
1993
From synapse to vesicle: The reuptake and storage of biogenic amine neurotransmitters
Rudnick G, Clark J. From synapse to vesicle: The reuptake and storage of biogenic amine neurotransmitters. Biochimica Et Biophysica Acta 1993, 1144: 249-263. PMID: 8104483, DOI: 10.1016/0005-2728(93)90109-s.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsPresynaptic plasma membraneBiogenic amine neurotransmittersEndogenous regulatory mechanismsSynaptic transmitter levelsPlasma membraneIndividual proteinsRegulatory mechanismsTransport systemMolecular levelSynaptic vesiclesAmine neurotransmittersIon gradientsTransportersVesiclesCDNARapid progressProteinNeurotransmittersRegulationMechanismMembraneSynapseTransmitter levels
1979
Mechanism of imipramine inhibition of platelet 5-hydroxytryptamine transport.
Talvenheimo J, Nelson P, Rudnick G. Mechanism of imipramine inhibition of platelet 5-hydroxytryptamine transport. Journal Of Biological Chemistry 1979, 254: 4631-4635. PMID: 438209, DOI: 10.1016/s0021-9258(17)30057-1.Peer-Reviewed Original Research
1976
Equilibrium between two forms of the lac carrier protein in energized and nonenergized membrane vesicles from Escherichia coli.
Rudnick G, Schuldiner S, Kaback H. Equilibrium between two forms of the lac carrier protein in energized and nonenergized membrane vesicles from Escherichia coli. Biochemistry 1976, 15: 5126-31. PMID: 791364, DOI: 10.1021/bi00668a028.Peer-Reviewed Original ResearchConceptsLac carrier proteinMembrane vesiclesCarrier proteinY gene productEscherichia coli ML 308P-nitrophenyl alphaD-lactateMembrane proteinsGene productsML 308Cryptic formVesicle membraneLactose transportElectrochemical gradientEscherichia coliEnergy couplingProteinVesiclesCarbonyl cyanideSimilar affinityCompetitive inhibitorHigh affinity formMembraneBindingFlow dialysis
1975
ACTIVE TRANSPORT IN ISOLATED BACTERIAL MEMBRANE VESICLES: BINDING OF β‐GALACTOSIDES TO THE LAC CARRIER PROTEIN
Kaback H, Rudnick G, Schuldiner S, Short S. ACTIVE TRANSPORT IN ISOLATED BACTERIAL MEMBRANE VESICLES: BINDING OF β‐GALACTOSIDES TO THE LAC CARRIER PROTEIN. Annals Of The New York Academy Of Sciences 1975, 264: 350-357. PMID: 769642, DOI: 10.1111/j.1749-6632.1975.tb31495.x.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements