1986
Characterization of native and reconstituted hydrogen ion pumping adenosinetriphosphatase of chromaffin granules.
Dean G, Nelson P, Rudnick G. Characterization of native and reconstituted hydrogen ion pumping adenosinetriphosphatase of chromaffin granules. Biochemistry 1986, 25: 4918-25. PMID: 2876726, DOI: 10.1021/bi00365a029.Peer-Reviewed Original ResearchConceptsChromaffin granule membranesATPase activityChromaffin granulesGranule membranesATP-dependent ion pumpsInhibitor sodium azidePlatelet dense granulesReconstituted membranesATP hydrolysisATP hydrolaseNucleotide dependenceAmine transportTransport ATPasesMitochondrial ATPaseAdrenal chromaffin granulesInhibitor sensitivityGranule acidificationIon pumpsSerotonin transportATPasesATPaseMonodisperse formATPOctyl glucosideNBD-Cl
1984
Spiperone: evidence for uptake into secretory granules.
Dannies P, Rudnick M, Fishkes H, Rudnick G. Spiperone: evidence for uptake into secretory granules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1867-1870. PMID: 6584920, PMCID: PMC345023, DOI: 10.1073/pnas.81.6.1867.Peer-Reviewed Original ResearchConceptsSerotonin receptorsSecretory granulesRat pituitary tumor cellsMicroM reserpinePlatelet dense granulesPituitary tumor cellsDopamine antagonistsIntracellular storage organellesSpiperoneTumor cellsSerotonin transportDense granulesBiogenic amine transportReceptorsCell strainsDopamineEquimolar concentrationsSpecific ligandsAmine transportStorage organellesPlasma membrane transport systemsReserpineAntagonistMinNeurons
1982
Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules.
Fishkes H, Rudnick G. Bioenergetics of serotonin transport by membrane vesicles derived from platelet dense granules. Journal Of Biological Chemistry 1982, 257: 5671-5677. PMID: 7068613, DOI: 10.1016/s0021-9258(19)83830-9.Peer-Reviewed Original ResearchConceptsPlatelet dense granulesMembrane vesiclesSerotonin transport activitySoluble enzymatic activityPresence of ATPCarbonyl cyanide p-trifluoromethoxyphenylhydrazoneAddition of ATPOsmotic lysatesTransport activityCyanide p-trifluoromethoxyphenylhydrazoneEnzymatic activityDensity gradient centrifugationVesiclesSerotonin transportVesicle interiorDense granulesMembrane potentialP-trifluoromethoxyphenylhydrazoneGradient centrifugationATPAssay mediumAdenine nucleotidesBioenergeticsNucleotidesGranules