2001
Factors that determine the unusually low reduction potential of cytochrome c550 in cyanobacterial photosystem II
Vrettos J, Reifler M, Kievit O, Lakshmi K, de Paula J, Brudvig G. Factors that determine the unusually low reduction potential of cytochrome c550 in cyanobacterial photosystem II. JBIC Journal Of Biological Inorganic Chemistry 2001, 6: 708-716. PMID: 11681704, DOI: 10.1007/s007750100249.Peer-Reviewed Original ResearchConceptsPCC 6803 photosystem IILow reduction potentialReduction potentialPyrolytic graphite edge electrodeElectron paramagnetic resonance spectroscopySquare wave voltammetryDirect electrochemical measurementsParamagnetic resonance spectroscopyBis-histidine axial ligationHeme reduction potentialCyanobacterial photosystem IIResonance Raman spectraPhotosystem IIWave voltammetryElectrode surfaceElectrochemistry experimentsElectrochemical measurementsElectrochemical valuesAxial ligationSolvent waterCyt c550Solvent exposureRedox titrationPeak separationPSII preparations
1996
Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase †
Sheptovitsky Y, Brudvig G. Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase †. Biochemistry 1996, 35: 16255-16263. PMID: 8973199, DOI: 10.1021/bi9613842.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsCatalaseCatechol OxidaseChloroplastsChromatography, High Pressure LiquidChromatography, Ion ExchangeCopperElectrophoresis, Polyacrylamide GelHot TemperatureIntracellular MembranesKineticsMolecular WeightOxygenOxygen ConsumptionPeptide MappingPhotosynthetic Reaction Center Complex ProteinsPhotosystem II Protein ComplexSpectrophotometrySpinacia oleraceaSubstrate SpecificityConceptsPSII membranesCentre of PSIIUV-visible absorptionO2 evolution activityThylakoid membranesSpinach thylakoid membranesAnion-exchange chromatographyMembrane-associated catalaseHeme enzymesBatch adsorptionHeme catalasesMild heat treatmentHeat treatmentMolecular weightAnion-exchange beadsNative molecular weightPPOIrreversible aggregationGel filtrationFurther manipulationAdsorptionAmino acid compositionTriazoleMembraneChromatography