2024
Molecular MR Imaging of T-Cell Immune Response to Cryoablation in Immunologically Hot vs. Cold Hepatocellular Carcinoma.
Santana J, Shewarega A, Nam D, Duncan J, Madoff D, Hyder F, Coman D, Chapiro J. Molecular MR Imaging of T-Cell Immune Response to Cryoablation in Immunologically Hot vs. Cold Hepatocellular Carcinoma. JHEP Reports 2024, 101294. DOI: 10.1016/j.jhepr.2024.101294.Peer-Reviewed Original ResearchT cell infiltrationHepatocellular carcinomaRadiological-pathological correlationImaging mass cytometryImmune responseT1-weighted MRITumor-infiltrating CD8+ T lymphocytesAnti-tumor immune responseCD8+ T lymphocytesIncreased T lymphocyte infiltrationImaging biomarkersNon-immunogenic tumorsSystemic lymph nodesT lymphocyte infiltrationMurine tumor modelsImmune cell typesLocal tumor therapyPrimary liver cancerNon-invasive imaging biomarkerTesla MRI scannerInduce liver cirrhosisImmunogenic tumorsLocoregional therapySystemic immunotherapyHCC lesionsComplexes of Iron(II), Cobalt(II), and Nickel(II) with DOTA-Tetraglycinate for pH and Temperature Imaging Using Hyperfine Shifts of an Amide Moiety
Mishra S, Zakaria A, Mihailovic J, Maritim S, Mercado B, Coman D, Hyder F. Complexes of Iron(II), Cobalt(II), and Nickel(II) with DOTA-Tetraglycinate for pH and Temperature Imaging Using Hyperfine Shifts of an Amide Moiety. Inorganic Chemistry 2024, 63: 22559-22571. PMID: 39533962, DOI: 10.1021/acs.inorgchem.4c04023.Peer-Reviewed Original ResearchParamagnetic complexesDivalent transition-metal ionsNitrogen donor atomsLanthanide metal ionsComplexes of iron(IITransition-metal ionsChemical exchange saturation transferDonor atomsAmide moietyCrystallographic dataCoordination numberHyperfine shiftsMetal ionsPH sensitivityMoietyProtonIonsLow cytotoxicityPH imagingSaturation transferMagnetic resonanceCobalt(IINickel(IIIron(IILanthanideTransient impairment in microglial function causes sex-specific deficits in synaptic maturity and hippocampal function in mice exposed to early adversity
Ahmed S, Polis B, Jamwal S, Sanganahalli B, MacDowell Kaswan Z, Islam R, Kim D, Bowers C, Giuliano L, Biederer T, Hyder F, Kaffman A. Transient impairment in microglial function causes sex-specific deficits in synaptic maturity and hippocampal function in mice exposed to early adversity. Brain Behavior And Immunity 2024, 122: 95-109. PMID: 39134183, PMCID: PMC11402597, DOI: 10.1016/j.bbi.2024.08.010.Peer-Reviewed Original ResearchEarly-life adversityModel of early-life adversityContextual fear conditioningLimited beddingFear conditioningSynaptic engulfmentLB miceSynaptic pruningSex-specific deficitsHippocampus of maleSex-specific effectsHippocampal deficitsAdolescent miceHippocampal functionEarly adversityChemogenetic activationSynaptic connectionsBehavioral aberrationsPotential compensatory mechanismsSynaptic abnormalitiesHippocampusAblation of microgliaRodent hippocampusDeficitsWeeks of lifemTOR inhibition enhances synaptic and mitochondrial function in Alzheimer’s disease in an APOE genotype-dependent manner
Sanganahalli B, Mihailovic J, Vekaria H, Coman D, Yackzan A, Flemister A, Aware C, Wenger K, Hubbard W, Sullivan P, Hyder F, Lin A. mTOR inhibition enhances synaptic and mitochondrial function in Alzheimer’s disease in an APOE genotype-dependent manner. Cerebrovascular And Brain Metabolism Reviews 2024, 44: 1745-1758. PMID: 38879800, PMCID: PMC11494852, DOI: 10.1177/0271678x241261942.Peer-Reviewed Original ResearchResponse to rapamycinE3FAD miceMitochondrial functionAlzheimer's diseaseMammalian target of rapamycinAD genetic risk factorsApolipoprotein E4Neuronal mitochondrial functionMitochondrial oxidative metabolismE4FAD miceHuman APOE4 geneTCA cycle rateGenetic risk factorsGenotype-dependent mannerE3FADTarget of rapamycinAPOE4 geneAPOE3 alleleGlutamate-glutamine cycleAPOE4 carriersBioenergetic measurementsE4FADMetabolic functionsAPOE genotypeMammalian target of rapamycin inhibitionFe(II), Co(II) and Ni(II) complexed DOTA-tetraglycinates as pH and temperature biosensors
Mishra S, Zakaria A, Mihailovic J, Maritim S, Coman D, Hyder F. Fe(II), Co(II) and Ni(II) complexed DOTA-tetraglycinates as pH and temperature biosensors. Proceedings Of The International Society For Magnetic Resonance In Medicine ... Scientific Meeting And Exhibition. 2024 DOI: 10.58530/2024/3207.Peer-Reviewed Original ResearchA Convolutional Neural Network Approach to Personalized Neuropil Density Prediction
Chang B, Akif A, Onofrey J, Hyder F. A Convolutional Neural Network Approach to Personalized Neuropil Density Prediction. Proceedings Of The International Society For Magnetic Resonance In Medicine ... Scientific Meeting And Exhibition. 2024 DOI: 10.58530/2024/4846.Peer-Reviewed Original ResearchConvolutional neural networkSuccess of machine learning methodsConvolutional neural network approachCell countNeural network approachMachine learning methodsNeural cell countNeural networkLearning methodsMetabolic imagingMulti-modal MRINetwork approachSynaptic densityPatient-specificBrain energeticsQuantify electrical activityNeural cellsEnergy budgetDensity predictionElectrical activityIn vivo neuropil density from anatomical MRI and machine learning
Akif A, Staib L, Herman P, Rothman D, Yu Y, Hyder F. In vivo neuropil density from anatomical MRI and machine learning. Cerebral Cortex 2024, 34: bhae200. PMID: 38771239, PMCID: PMC11107380, DOI: 10.1093/cercor/bhae200.Peer-Reviewed Original ResearchConceptsMagnetic resonance imagingSynaptic densityNeuropil densityCellular densityArtificial neural networkNeural networkPositron emission tomographyAnatomical magnetic resonance imagingHealthy subjectsSynaptic activityMRI scansMachine learning algorithmsBrain's energy budgetEmission tomographyIn vivo MRI scansResonance imagingTissue cellularityLearning algorithmsDiffusion magnetic resonance imagingMachine learningMicroscopic interpretationInterpretation of functional neuroimaging dataIndividual predictionsSubjectsMacro- and microvascular contributions to cerebral structural alterations in patients with asymptomatic carotid artery stenosis
Schmitzer L, Kaczmarz S, Göttler J, Hoffmann G, Kallmayer M, Eckstein H, Hedderich D, Kufer J, Zimmer C, Preibisch C, Hyder F, Sollmann N. Macro- and microvascular contributions to cerebral structural alterations in patients with asymptomatic carotid artery stenosis. Cerebrovascular And Brain Metabolism Reviews 2024, 44: 1629-1642. PMID: 38506325, PMCID: PMC11418673, DOI: 10.1177/0271678x241238935.Peer-Reviewed Original ResearchInternal carotid artery stenosisInternal carotid artery stenosis patientsCerebral blood flowCarotid artery stenosisPeak width of skeletonized mean diffusivitySmall vessel diseaseContralateral alterationsArtery stenosisAsymptomatic internal carotid artery stenosisWhite matterAsymptomatic carotid artery stenosisCapillary transit time heterogeneityCerebral structural alterationsCortical thicknessMagnetic resonance imagingHemodynamic alterationsRisk factorsPatientsDTI metricsResonance imagingDorsal cingulate cortexBlood flowIschemic strokeMean diffusivityStenosisHigh‐resolution extracellular pH imaging of liver cancer with multiparametric MR using Deep Image Prior
Dong S, Shewarega A, Chapiro J, Cai Z, Hyder F, Coman D, Duncan J. High‐resolution extracellular pH imaging of liver cancer with multiparametric MR using Deep Image Prior. NMR In Biomedicine 2024, 37: e5145. PMID: 38488205, DOI: 10.1002/nbm.5145.Peer-Reviewed Original ResearchDeep Image PriorU-NetUnsupervised deep learning techniquesU-Net parametersDeep learning techniquesHigh-resolution ground truthU-Net architectureSuper-resolution imagingImage priorsSuper-resolutionGround truthMean absolute errorDeepSpatial resolutionPostprocessing methodDeep imagingAbsolute errorImagesAnatomical MR imagesMR spectroscopic imagingAnatomical informationSpectroscopic imagingInformationAcquisition timeErrorVEGF-C prophylaxis favors lymphatic drainage and modulates neuroinflammation in a stroke model
Boisserand L, Geraldo L, Bouchart J, Kamouh M, Lee S, Sanganahalli B, Spajer M, Zhang S, Lee S, Parent M, Xue Y, Skarica M, Yin X, Guegan J, Boyé K, Leser F, Jacob L, Poulet M, Li M, Liu X, Velazquez S, Singhabahu R, Robinson M, Askenase M, Osherov A, Sestan N, Zhou J, Alitalo K, Song E, Eichmann A, Sansing L, Benveniste H, Hyder F, Thomas J. VEGF-C prophylaxis favors lymphatic drainage and modulates neuroinflammation in a stroke model. Journal Of Experimental Medicine 2024, 221: e20221983. PMID: 38442272, PMCID: PMC10913814, DOI: 10.1084/jem.20221983.Peer-Reviewed Original ResearchConceptsVascular endothelial growth factor-CDeep cervical lymph nodesCentral nervous systemEffect of vascular endothelial growth factor-CMeningeal lymphatic vesselsAmeliorated motor performanceCervical lymph nodesIschemic strokeVEGF-C overexpressionIncreased BDNF signalingAcute ischemic strokeBrain cellsIncreased CSF drainageIschemic stroke outcomesModel of ischemic strokeMouse model of ischemic strokeImmune surveillanceCSF drainageLymph nodesFluid drainageNucleus RNA sequencingLymphatic growthLymphatic drainageMouse modelBDNF signalingTransmembrane pH gradient imaging in rodent glioma models
Mishra S, Santana J, Mihailovic J, Hyder F, Coman D. Transmembrane pH gradient imaging in rodent glioma models. NMR In Biomedicine 2024, 37: e5102. PMID: 38263680, PMCID: PMC10987279, DOI: 10.1002/nbm.5102.Peer-Reviewed Original ResearchNormal tissuesRodent glioma modelsGL261 gliomasU87 gliomasTumor microenvironmentPotential therapeutic targetGlioma modelTumor survivalExtracellular acidosisTumorMetabolic reprogrammingRegulate drug deliveryIntracellular pHRat brainExtracellular pHTherapeutic targetGliomaMouse brainDrug deliveryIntracellular milieuTransmembrane pH gradientBrainSubmillimeter resolutionTissueCellsMultimodal measures of spontaneous brain activity reveal both common and divergent patterns of cortical functional organization
Vafaii H, Mandino F, Desrosiers-Grégoire G, O’Connor D, Markicevic M, Shen X, Ge X, Herman P, Hyder F, Papademetris X, Chakravarty M, Crair M, Constable R, Lake E, Pessoa L. Multimodal measures of spontaneous brain activity reveal both common and divergent patterns of cortical functional organization. Nature Communications 2024, 15: 229. PMID: 38172111, PMCID: PMC10764905, DOI: 10.1038/s41467-023-44363-z.Peer-Reviewed Original Research
2023
Cross-hemicord spinal fiber reorganization associates with cortical sensory and motor network expansion in the rat model of hemicontusion cervical spinal cord injury
Mihailovic J, Sanganahalli B, Hyder F, Chitturi J, Elkabes S, Heary R, Kannurpatti S. Cross-hemicord spinal fiber reorganization associates with cortical sensory and motor network expansion in the rat model of hemicontusion cervical spinal cord injury. Neuroscience Letters 2023, 820: 137607. PMID: 38141752, PMCID: PMC10797561, DOI: 10.1016/j.neulet.2023.137607.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCervical spinal cord injuryDiffusion tensor imagingCord injurySpinal cordCervical spinal cordState functional connectivitySpinal cord samplesSpinal cord diffusion tensor imagingMagnetic resonance imagingBrain functional MRIInjury epicenterSpinal reorganizationPost-SCINeuroplastic changesCord samplesCortical sensoryRat modelTraumatic injuryMD changeMotor networkNervous systemResonance imagingSingle-subject levelFunctional MRIA multi-laboratory preclinical trial in rodents to assess treatment candidates for acute ischemic stroke
Lyden P, Diniz M, Bosetti F, Lamb J, Nagarkatti K, Rogatko A, Kim S, Cabeen R, Koenig J, Akhter K, Arbab A, Avery B, Beatty H, Bibic A, Cao S, Simoes Braga Boisserand L, Chamorro A, Chauhan A, Diaz-Perez S, Dhandapani K, Dhanesha N, Goh A, Herman A, Hyder F, Imai T, Johnson C, Khan M, Kamat P, Karuppagounder S, Kumskova M, Mihailovic J, Mandeville J, Morais A, Patel R, Sanganahalli B, Smith C, Shi Y, Sutariya B, Thedens D, Qin T, Velazquez S, Aronowski J, Ayata C, Chauhan A, Leira E, Hess D, Koehler R, McCullough L, Sansing L. A multi-laboratory preclinical trial in rodents to assess treatment candidates for acute ischemic stroke. Science Translational Medicine 2023, 15: eadg8656. PMID: 37729432, DOI: 10.1126/scitranslmed.adg8656.Peer-Reviewed Original ResearchConceptsPreclinical assessmentFocal cerebral ischemic insultAcute ischemic strokeCerebral ischemic insultLarge clinical trialsDiet-induced obesityNew clinical interventionsIntravascular thrombectomyIschemic strokeStroke treatmentHypertensive ratsIschemic insultBlinded assessmentClinical trialsYoung miceTreatment candidatesExclusion criteriaAnimal modelsYoung ratsFutile interventionsPreclinical trialsClinical interventionsFutility boundariesMiceDisease areasSpatiotemporal features of neurovascular (un)coupling with stimulus-induced activity and hypercapnia challenge in cerebral cortex and olfactory bulb
James S, Sanggaard S, Akif A, Mishra S, Sanganahalli B, Blumenfeld H, Verhagen J, Hyder F, Herman P. Spatiotemporal features of neurovascular (un)coupling with stimulus-induced activity and hypercapnia challenge in cerebral cortex and olfactory bulb. Cerebrovascular And Brain Metabolism Reviews 2023, 43: 1891-1904. PMID: 37340791, PMCID: PMC10676132, DOI: 10.1177/0271678x231183887.Peer-Reviewed Original ResearchConceptsVasodilatory responseCerebral cortexNeurovascular couplingOlfactory bulbNeuronal activityBrief sensory stimuliRegional neurovascular couplingStimulus-induced activityHypercapnia challengeVascular toneNeuronal deactivationHemodynamic responseNeuronal excitabilityNeuronal responsesCalcium transientsBrain functionHemodynamic signalsSensory stimuliVasodilationHypercapniaCortexMiceCareful appraisalStimuliMetabolic wasteNeurovascular coupling is optimized to compensate for the increase in proton production from nonoxidative glycolysis and glycogenolysis during brain activation and maintain homeostasis of pH, pCO2, and pO2
DiNuzzo M, Dienel G, Behar K, Petroff O, Benveniste H, Hyder F, Giove F, Michaeli S, Mangia S, Herculano‐Houzel S, Rothman D. Neurovascular coupling is optimized to compensate for the increase in proton production from nonoxidative glycolysis and glycogenolysis during brain activation and maintain homeostasis of pH, pCO2, and pO2. Journal Of Neurochemistry 2023, 168: 632-662. PMID: 37150946, PMCID: PMC10628336, DOI: 10.1111/jnc.15839.Peer-Reviewed Original ResearchHigher cerebral blood flowCerebral blood flowOxygen extraction fractionNeurovascular couplingBlood flowLower oxygen extraction fractionNonoxidative glycolysisCerebral metabolic rateBrain pHCapillary densityNeurovascular diseasesNonoxidative metabolismTissue oxygenationHuman cortexBrain functionExtraction fractionBrain activationMitochondrial ATP productionPET measurementsCMROOxygen consumptionHomeostasisGlycogenolysisFMRI contrastActivationMultivariate analysis of perfusion and oxygenation patterns in asymptomatic unilateral carotid artery stenosis
Kufer J, Göttler J, Hoffmann G, Schmitzer L, Zimmer C, Hyder F, Preibisch C, Kaczmarz S. Multivariate analysis of perfusion and oxygenation patterns in asymptomatic unilateral carotid artery stenosis. Proceedings Of The International Society For Magnetic Resonance In Medicine ... Scientific Meeting And Exhibition. 2023 DOI: 10.58530/2023/0878.Peer-Reviewed Original ResearchInternal carotid artery stenosisCarotid artery stenosisArtery stenosisInternal carotid artery stenosis patientsAsymptomatic internal carotid artery stenosisUnilateral carotid artery stenosisSystemic blood pressureAnalysis of perfusionIncreased stroke riskCerebral hemodynamic changesDegree of stenosisPhysiologic MRICerebral perfusionComplex pathophysiologyMultivariate analysisHemodynamic changesBlood pressureCarotid arteryTreatment decisionsStenosisDisease-specific patternsStroke riskImaging the transmembrane pH gradient in gliomas
Mishra S, Mihailović J, Hyder F, Coman D. Imaging the transmembrane pH gradient in gliomas. Proceedings Of The International Society For Magnetic Resonance In Medicine ... Scientific Meeting And Exhibition. 2023 DOI: 10.58530/2023/3928.Peer-Reviewed Original ResearchAssess tumor aggressivenessMonitoring treatment efficacyTransmembrane pH gradientTumor responseTumor microenvironmentTumor aggressivenessRG2 tumorsLocal drug deliveryExtracellular acidosisTherapeutic outcomesTumorTreatment efficacyNormal tissuesBrain tumorsIntracellular pHExtracellular pHFunction biomarkersPositive therapeutic outcomesDrug deliveryIntracellular milieuBrainAcidosisGlioma430. Calibrated fMRI Sensitive to Ketamine-Related Increases Throughout the Cortex
Driesen N, Herman P, Rowland M, Thompson G, Qiu M, He G, Criscuolo E, Fineberg S, Barron D, Helgeson L, Lacadie C, Chow R, Gueorguieva R, Straun T, Krystal J, Hyder F. 430. Calibrated fMRI Sensitive to Ketamine-Related Increases Throughout the Cortex. Biological Psychiatry 2023, 93: s268-s269. DOI: 10.1016/j.biopsych.2023.02.670.Peer-Reviewed Original ResearchComputational Image-Based Stroke Assessment for Evaluation of Cerebroprotectants with Longitudinal and Multi-Site Preclinical MRI
Cabeen R, Mandeville J, Hyder F, Sanganahalli B, Thedens D, Arbab A, Huang S, Bibic A, Tarakci E, Mihailovic J, Morais A, Lamb J, Nagarkatti K, Toga A, Lyden P, Ayata C. Computational Image-Based Stroke Assessment for Evaluation of Cerebroprotectants with Longitudinal and Multi-Site Preclinical MRI. 2023, 00: 1-5. DOI: 10.1109/isbi53787.2023.10230408.Peer-Reviewed Original ResearchMiddle cerebral artery occlusionData analytics approachManual annotationStroke assessmentRodent modelsCerebral artery occlusionData qualityCoronal MRI slicesPotential therapeutic interventionsMRI slicesNetworkAssessment NetworkOutcome quantificationInfarct volumeArtery occlusionIschemic strokeStroke outcomeBrain atrophyMidline shiftMRI dataLeading causeRodent preclinical studiesStroke expertsPreclinical studiesLesion extent