2012
Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices.
Popovic M, Gao X, Zecevic D. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices. Journal Of Visualized Experiments 2012, e4261. PMID: 23222505, PMCID: PMC3565854, DOI: 10.3791/4261.Peer-Reviewed Original ResearchConceptsLaser light sourceSub-micrometer resolutionLight sourceOptical recordingDetectable pharmacological effectsCurrent sensitivityVoltage-sensitive dyeSubthreshold eventsElectrical propertiesDetailed measurementsMultiple-site optical recordingVoltage-sensitive dye recordingFluorescence signalOptimal wavelengthsMembrane potential transientsElectrical signalsIndividual dendritic spinesExperimental evidenceWavelengthMeasurement oneMeasurementsBroadeningDye loadingPotential transientsBrightness
2009
Imaging Membrane Potential with Voltage-Sensitive Dyes
Vucinic D, Kosmidis E, Falk C, Cohen L, Loew L, Djurisic M, Zecevic D. Imaging Membrane Potential with Voltage-Sensitive Dyes. 2009, 132-160. DOI: 10.1093/oso/9780195150445.003.0006.Peer-Reviewed Original ResearchMembrane-bound dyeOptical signalSecond harmonic generationVoltage-sensitive dyeDifferent optical propertiesHarmonic generationOptical measurementsOptical propertiesRaman absorptionOptical recordingRise timeSimultaneous measurementAbsorptionFigure 6.1BirefringenceFluorescenceMeasurementsDichroismDyeMolecular probesSignalsSquidKinds of results
2005
Imaging Brain Activity With Voltage- and Calcium-Sensitive Dyes
Baker BJ, Kosmidis EK, Vucinic D, Falk CX, Cohen LB, Djurisic M, Zecevic D. Imaging Brain Activity With Voltage- and Calcium-Sensitive Dyes. Cellular And Molecular Neurobiology 2005, 25: 245-282. PMID: 16050036, DOI: 10.1007/s10571-005-3059-6.Peer-Reviewed Original ResearchConceptsVoltage-sensitive dyeCalcium-sensitive dyeLight sourceDark noiseVibrational noiseOptical recordingRandom emissionBrain activityFluorescent protein sensorOlfactory receptor neuronsNerve terminalsOlfactory bulbSpike activityNoise ratioCell bodiesReceptor neuronsPhotonsOpticsOptimal signalNeuronsIndividual cell typesKinds of noiseExternal sourcesVoltageCell types
2003
Imaging Nervous System Activity with Voltage‐Sensitive Dyes
Zecevic D, Djurisic M, Cohen LB, Antic S, Wachowiak M, Falk CX, Zochowski MR. Imaging Nervous System Activity with Voltage‐Sensitive Dyes. Current Protocols In Neuroscience 2003, 23: 6.17.1-6.17.29. PMID: 18428582, DOI: 10.1002/0471142301.ns0617s23.Peer-Reviewed Original ResearchConceptsNumber of photonsVoltage-sensitive dyeVoltage-sensitive dye measurementsLight sourceShot noiseOptical recordingOptical recording methodSynaptic inputsChoice of dyeSimultaneous measurementNervous system activityAction potential outputAplysia abdominal ganglionLarge signalNoise ratioAbdominal ganglionNervous systemPhotonsDye measurementsOpticsMotor outputSystem activitySensory stimuliMeasurementsVertebrate brain
1999
Fast Optical Recordings of Membrane Potential Changes From Dendrites of Pyramidal Neurons
Antic S, Major G, Zecevic D. Fast Optical Recordings of Membrane Potential Changes From Dendrites of Pyramidal Neurons. Journal Of Neurophysiology 1999, 82: 1615-1621. PMID: 10482775, DOI: 10.1152/jn.1999.82.3.1615.Peer-Reviewed Original ResearchConceptsBasolateral dendritesApical dendritesBrain slicesVoltage-sensitive dyeNeuronal processesPyramidal neuronsMembrane potential transientsPharmacological effectsSynaptic stimulationAction potentialsInvertebrate neuronsNeuronsPatch electrodeSingle neuronsElectrical activityMembrane potential changesFast optical recordingDistal processesVertebrate neuronsOptical recordingDendritesH incubation periodStimulationApical processesRecordingsChapter sixteen Imaging Membrane Potential Changes in Individual Neurons
Antić S, Zečević D. Chapter sixteen Imaging Membrane Potential Changes in Individual Neurons. 1999, 238-248. DOI: 10.1016/b978-012447836-7/50018-x.Peer-Reviewed Original ResearchVoltage-sensitive dyeIndividual neuronsSingle neuronsVoltage-sensitive dye recordingSpatial resolutionIndirect optical measurementsDirect electrical recordingOptical signalGood spatial resolutionOptical measurementsIndividual nerve cellsPresent sensitivityFunctional neuronal networksNeuronal dendritic treeOptical recordingDendritic treeNerve cellsDye injectionNeuronsIntracellular applicationMembrane potential changesNeuronal networksMultisite recordingsElectrical recordingsLocal modulation
1992
6 Many neurones in the Aplysia abdominal ganglion are active during the gill-withdrawal reflex
Falk C, Wu J, Cohen L, Hopp H, Zecevic D, London J, Roschin V. 6 Many neurones in the Aplysia abdominal ganglion are active during the gill-withdrawal reflex. 1992, 88-101. DOI: 10.1016/b978-0-08-041986-2.50011-4.Peer-Reviewed Original ResearchAplysia abdominal ganglionGill withdrawal reflexAbdominal ganglionActive neuronsAction potential activityNumber of spikesGenital nerveOptical recording methodVoltage-sensitive dyeGill withdrawalNeuron activityNervous tissueGangliaSensitized stateNeuronsInvertebrate gangliaAplysia californicaReflexExtracellular electrodesOptical recordingPotential activityRecording methodNerveInterneuronsRecordings
1989
Optical recording of membrane potential at the single cell level
Cohen L, Hoepp H, Wu J, Xiao C, Zecevic D, London J. Optical recording of membrane potential at the single cell level. 1989, wd2. DOI: 10.1364/oam.1989.wd2.Peer-Reviewed Original ResearchExcellent time resolutionOptical signalsOptical recordings of membrane potentialGill withdrawal reflexMolecular rotationTime resolutionOptical measurementsRecordings of membrane potentialMembrane potentialNervous systemOptical propertiesAction potential activityPhotodiode arrayOptical recordingActivity of neuronsAbdominal ganglionSimultaneous measurement
1987
Simultaneous optical recording of activity from many neurons during feeding in Navanax
London JA, Zecević D, Cohen LB. Simultaneous optical recording of activity from many neurons during feeding in Navanax. Journal Of Neuroscience 1987, 7: 649-661. PMID: 2435862, PMCID: PMC6569073, DOI: 10.1523/jneurosci.07-03-00649.1987.Peer-Reviewed Original ResearchOptical recording of action potential propagation in demyelinated frog nerve
Shrager P, Chiu SY, Ritchie JM, Zecevic D, Cohen LB. Optical recording of action potential propagation in demyelinated frog nerve. Biophysical Journal 1987, 51: 351-355. PMID: 3493812, PMCID: PMC1329897, DOI: 10.1016/s0006-3495(87)83342-8.Peer-Reviewed Original Research