2017
Identification of RUNX1 as a Mediator of Aberrant Retinal Angiogenesis
Lam JD, Oh DJ, Wong LL, Amarnani D, Park-Windhol C, Sanchez AV, Cardona-Velez J, McGuone D, Stemmer-Rachamimov AO, Eliott D, Bielenberg DR, van Zyl T, Shen L, Gai X, D'Amore PA, Kim LA, Arboleda-Velasquez JF. Identification of RUNX1 as a Mediator of Aberrant Retinal Angiogenesis. Diabetes 2017, 66: db161035. PMID: 28400392, PMCID: PMC5482092, DOI: 10.2337/db16-1035.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAnimalsCell MovementCell ProliferationCore Binding Factor Alpha 2 SubunitDiabetes Mellitus, Type 1Diabetes Mellitus, Type 2Diabetic RetinopathyDisease Models, AnimalEndothelial CellsFemaleGlucoseHumansImmunohistochemistryMaleMiceMiddle AgedOxygenRetinaRetinal NeovascularizationRNA, MessengerConceptsAberrant retinal angiogenesisHuman retinal microvascular endothelial cellsProliferative diabetic retinopathyOxygen-induced retinopathyFibrovascular membranesRetinal angiogenesisEndothelial cellsRetinal microvascular endothelial cellsType 2 diabetesRetina of miceMicrovascular endothelial cellsVascular endothelial cellsNeovascular tuftsDiabetic retinopathyTranscription factor 1Common causeRUNX1 inhibitionImmunohistochemical stainingAdult populationHigh glucoseType 1RetinopathyProtein expressionTube formationFactor 1
2015
Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy.
Kim LA, Wong LL, Amarnani DS, Bigger-Allen AA, Hu Y, Marko CK, Eliott D, Shah VA, McGuone D, Stemmer-Rachamimov AO, Gai X, D'Amore PA, Arboleda-Velasquez JF. Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy. Molecular Vision 2015, 21: 673-87. PMID: 26120272, PMCID: PMC4462955.Peer-Reviewed Original ResearchConceptsComparative genomic hybridizationDiscovery of cellsPlasma membrane infoldingsSpecific cell populationsCell identityPrimary culturesCell culture modelCharacterization of cellsMajor chromosomal aberrationsMembrane infoldingsCellular constituentsGenomic hybridizationAlpha-smooth muscle actinThrombospondin-1Cell populationsExpression of markersCulture conditionsStromal cellsPerivascular cellsCulture modelUnique resourceGlial fibrillary acidic protein-positive cellsCellsChromosomal aberrationsEndothelial cells