Identification of neurotoxic cytokines by profiling Alzheimer’s disease tissues and neuron culture viability screening
Wood LB, Winslow AR, Proctor EA, McGuone D, Mordes DA, Frosch MP, Hyman BT, Lauffenburger DA, Haigis KM. Identification of neurotoxic cytokines by profiling Alzheimer’s disease tissues and neuron culture viability screening. Scientific Reports 2015, 5: 16622. PMID: 26564777, PMCID: PMC4643219, DOI: 10.1038/srep16622.Peer-Reviewed Original ResearchMeSH KeywordsAgedAged, 80 and overAlzheimer DiseaseAmyloid beta-PeptidesBrainCell SurvivalCells, CulturedCytokinesFemaleGene Expression ProfilingGene Regulatory NetworksHumansInflammation MediatorsInterleukin-5Least-Squares AnalysisMaleMiddle AgedMultivariate AnalysisNeuronsRegression AnalysisTumor Necrosis Factor-alphaVascular Endothelial Growth Factor AConceptsCytokine expressionNeuronal deathNeuron culturesAD-associated cytokinesPathologic disease statesDisease tissuesControl brain tissueAlzheimer's disease tissueDisease therapeuticsAlzheimer's disease therapeuticsNeurotoxic cytokinesPro-death effectAD pathogenesisNeuronal viabilityAD tissueAmyloid betaAmyloid hypothesisMinimal efficacyHuman ADSmall cohortPathological severityCytokinesBrain tissueDisease statesVEGFCharacterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy.
Kim LA, Wong LL, Amarnani DS, Bigger-Allen AA, Hu Y, Marko CK, Eliott D, Shah VA, McGuone D, Stemmer-Rachamimov AO, Gai X, D'Amore PA, Arboleda-Velasquez JF. Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy. Molecular Vision 2015, 21: 673-87. PMID: 26120272, PMCID: PMC4462955.Peer-Reviewed Original ResearchConceptsComparative genomic hybridizationDiscovery of cellsPlasma membrane infoldingsSpecific cell populationsCell identityPrimary culturesCell culture modelCharacterization of cellsMajor chromosomal aberrationsMembrane infoldingsCellular constituentsGenomic hybridizationAlpha-smooth muscle actinThrombospondin-1Cell populationsExpression of markersCulture conditionsStromal cellsPerivascular cellsCulture modelUnique resourceGlial fibrillary acidic protein-positive cellsCellsChromosomal aberrationsEndothelial cells