Studying synapses in human brain with array tomography and electron microscopy
Kay KR, Smith C, Wright AK, Serrano-Pozo A, Pooler AM, Koffie R, Bastin ME, Bak TH, Abrahams S, Kopeikina KJ, McGuone D, Frosch MP, Gillingwater TH, Hyman BT, Spires-Jones TL. Studying synapses in human brain with array tomography and electron microscopy. Nature Protocols 2013, 8: 1366-1380. PMID: 23787894, PMCID: PMC3712649, DOI: 10.1038/nprot.2013.078.Peer-Reviewed Original ResearchConceptsHuman brainClinicopathological correlationArray tomographySynapse densityPostmortem studiesPsychiatric illnessClinical cohortAutopsy tissueBrain bankingThousands of synapsesNeurodegenerative diseasesSynapsesUltrastructural studySerial sectionsSynapse levelHuman samplesDiseaseBrainDevelopmental diseasesTomographyLight microscopyUltrathin serial sectionsCohortIllnessPotent way