2018
Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain
Dave JM, Mirabella T, Weatherbee SD, Greif DM. Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain. Developmental Cell 2018, 44: 665-678.e6. PMID: 29456135, PMCID: PMC5871595, DOI: 10.1016/j.devcel.2018.01.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood-Brain BarrierBrainEmbryo, MammalianEndothelium, VascularFemaleHumansIntracranial HemorrhagesMaleMiceMice, Inbred C57BLMice, KnockoutMorphogenesisPericytesProtein Serine-Threonine KinasesReceptor, Transforming Growth Factor-beta Type IReceptors, Transforming Growth Factor betaSignal TransductionTissue Inhibitor of Metalloproteinase-3ConceptsEndothelial morphogenesisReceptor-like kinasesBlood-brain barrierActivin receptor-like kinaseGerminal matrixGerminal matrix hemorrhage-intraventricular hemorrhageMurine embryosEC morphogenesisEndothelial cellsEC hyperproliferationMorphogenesisMatrix metalloproteinase-3Glial precursorsBasement membrane collagenMatrix metalloproteinase activityBBB developmentMutantsVascular stabilityALK5Brain pericytesAbnormal dilationSubstantial therapeutic potentialMetalloproteinase-3Membrane collagenTissue inhibitor
2013
Development and pathologies of the arterial wall
Seidelmann SB, Lighthouse JK, Greif DM. Development and pathologies of the arterial wall. Cellular And Molecular Life Sciences 2013, 71: 1977-1999. PMID: 24071897, PMCID: PMC11113178, DOI: 10.1007/s00018-013-1478-y.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenic ProteinsAnimalsArteriesCardiovascular DiseasesCell DifferentiationCell LineageEndothelial CellsEndothelium, VascularGene Expression Regulation, DevelopmentalHumansMorphogenesisMuscle, Smooth, VascularMyocytes, Smooth MuscleNeovascularization, PathologicNeovascularization, PhysiologicConceptsAdventitial progenitor cellsDevastating vascular diseaseDevelopmental biologyWall developmentSmooth muscle cell originHuman diseasesExtracellular matrixMuscle cell originProcess of angiogenesisProgenitor cellsEndothelial networksDisease mechanismsNovel therapeutic strategiesDevelopmental studiesEndothelial cellsSmooth muscleCellsMorphogenesisTherapeutic strategiesCell originBiologyDifferentiationVascular diseaseVascular wallVascular wall abnormalities
2010
Targeting Robo4-Dependent Slit Signaling to Survive the Cytokine Storm in Sepsis and Influenza
London NR, Zhu W, Bozza FA, Smith MC, Greif DM, Sorensen LK, Chen L, Kaminoh Y, Chan AC, Passi SF, Day CW, Barnard DL, Zimmerman GA, Krasnow MA, Li DY. Targeting Robo4-Dependent Slit Signaling to Survive the Cytokine Storm in Sepsis and Influenza. Science Translational Medicine 2010, 2: 23ra19. PMID: 20375003, PMCID: PMC2875996, DOI: 10.1126/scitranslmed.3000678.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCadherinsCapillary PermeabilityCateninsCell LineCytokinesDelta CateninDisease Models, AnimalEndothelium, VascularHumansInfluenza A Virus, H5N1 SubtypeIntercellular Signaling Peptides and ProteinsLipopolysaccharidesMiceMice, Inbred C57BLNerve Tissue ProteinsOrthomyxoviridae InfectionsProtein BindingProtein StabilityProtein TransportReceptors, Cell SurfaceReceptors, ImmunologicSepsisSignal TransductionConceptsInnate immune responseImmune systemCytokine stormImmune responseInfectious agentsHost innate immune responseCurrent medical therapyMultiple inflammatory cytokinesBacterial endotoxin exposureHost immune systemInnate immune systemMultiple infectious agentsAdditional immune responsesPolymicrobial sepsisMedical therapyOrgan failureInflammatory cytokinesCytokine releaseTumor necrosisEndotoxin exposureTissue edemaInterleukin-1betaVascular permeabilityTherapeutic strategiesAnimal models
2004
KLF2 Is a Novel Transcriptional Regulator of Endothelial Proinflammatory Activation
SenBanerjee S, Lin Z, Atkins GB, Greif DM, Rao RM, Kumar A, Feinberg MW, Chen Z, Simon DI, Luscinskas FW, Michel TM, Gimbrone MA, García-Cardeña G, Jain MK. KLF2 Is a Novel Transcriptional Regulator of Endothelial Proinflammatory Activation. Journal Of Experimental Medicine 2004, 199: 1305-1315. PMID: 15136591, PMCID: PMC2211816, DOI: 10.1084/jem.20031132.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCells, CulturedDNA PrimersEndothelium, VascularE-SelectinGene Expression RegulationGlutathione TransferaseHumansInflammationIntercellular Adhesion Molecule-1Interleukin-1Kruppel-Like Transcription FactorsNF-kappa BNitric Oxide SynthaseNitric Oxide Synthase Type IIIOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionPromoter Regions, GeneticStress, MechanicalTrans-ActivatorsTranscription, GeneticUmbilical VeinsZinc FingersConceptsUmbilical vein endothelial cellsVein endothelial cellsProinflammatory cytokinesEndothelial nitric oxide synthase expressionVascular cell adhesion molecule-1Nitric oxide synthase expressionInflammatory cytokines interleukin-1betaEndothelial cellsCell adhesion molecule-1Endothelial proinflammatory activationOxide synthase expressionCultured human umbilical vein endothelial cellsCytokine interleukin-1betaVascular disease statesEndothelial adhesion molecules EAdhesion molecule-1Adhesion molecules EOverexpression of KLF2Human umbilical vein endothelial cellsAMP response element binding proteinCyclic AMP response element binding proteinResponse element-binding proteinT cell attachmentEndothelial functionEndothelial activation
2002
Dephosphorylation of Endothelial Nitric-oxide Synthase by Vascular Endothelial Growth Factor IMPLICATIONS FOR THE VASCULAR RESPONSES TO CYCLOSPORIN A*
Kou R, Greif D, Michel T. Dephosphorylation of Endothelial Nitric-oxide Synthase by Vascular Endothelial Growth Factor IMPLICATIONS FOR THE VASCULAR RESPONSES TO CYCLOSPORIN A*. Journal Of Biological Chemistry 2002, 277: 29669-29673. PMID: 12050171, DOI: 10.1074/jbc.m204519200.Peer-Reviewed Original ResearchConceptsENOS dephosphorylationSerine 116MAP kinase pathway inhibitor U0126Protein phosphatase pathwaysPhosphorylation-deficient mutantDependent protein phosphatasePhosphorylation state-specific antibodiesWild-type enzymeVascular endothelial growth factorProtein kinase C inhibitor calphostinPathway inhibitor U0126Activation of calcineurinProtein phosphataseInhibitor of calcineurinPhosphatase pathwaysENOS agonistsProtein kinaseEndothelial cellsAlanine residuesDephosphorylationDependent enzymesInhibitor U0126Dephosphorylation of eNOSCultured endothelial cellsEnzyme activity
1997
Isolated P-selectin Glycoprotein Ligand-1 Dynamic Adhesion to P- and E-selectin
Goetz D, Greif D, Ding H, Camphausen R, Howes S, Comess K, Snapp K, Kansas G, Luscinskas F. Isolated P-selectin Glycoprotein Ligand-1 Dynamic Adhesion to P- and E-selectin. Journal Of Cell Biology 1997, 137: 509-519. PMID: 9128259, PMCID: PMC2139768, DOI: 10.1083/jcb.137.2.509.Peer-Reviewed Original ResearchAnimalsCell AdhesionCHO CellsCricetinaeEndothelium, VascularE-SelectinHumansImmunoglobulin Fc FragmentsLigandsMembrane GlycoproteinsMetalloendopeptidasesMicrospheresNeuraminidaseOligosaccharidesP-SelectinRecombinant Fusion ProteinsSialyl Lewis X AntigenThreonineTumor Necrosis Factor-alphaUmbilical Veins