2024
Rab6a enables BICD2/dynein-mediated trafficking of human papillomavirus from the trans-Golgi network during virus entry
Choi J, Speckhart K, Tsai B, DiMaio D. Rab6a enables BICD2/dynein-mediated trafficking of human papillomavirus from the trans-Golgi network during virus entry. MBio 2024, 15: e02811-24. PMID: 39431827, PMCID: PMC11559006, DOI: 10.1128/mbio.02811-24.Peer-Reviewed Original ResearchRab proteinsDNA virusesVirus entryHPV traffickingIntra-Golgi transportTrans-Golgi networkIntracellular vesicular transportMotor protein complexInfected cellsIntra-GolgiL2 capsid proteinRab GTPasesDynein adaptorsNon-enveloped DNA virusesRetrograde traffickingGolgi apparatusPotential targetProtein complexesVesicular transportRab6ADyneinTGNCapsid proteinRabCellular enzymesThe BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection
Speckhart K, Choi J, DiMaio D, Tsai B. The BICD2 dynein cargo adaptor binds to the HPV16 L2 capsid protein and promotes HPV infection. PLOS Pathogens 2024, 20: e1012289. PMID: 38829892, PMCID: PMC11230635, DOI: 10.1371/journal.ppat.1012289.Peer-Reviewed Original ResearchTrans Golgi networkL2 capsid proteinPromote infectionCapsid proteinIn vitro binding studiesDynein adaptorsGolgi networkCargo adaptorsBICD2 functionDynein motorsC-terminusDyneinSupport infectionMolecular basisBICD2EndosomesAdaptorProteinBinding studiesCell-basedVirusShort segmentHuman papillomavirusGolgiInfectionDe novo-designed transmembrane proteins bind and regulate a cytokine receptor
Mravic M, He L, Kratochvil H, Hu H, Nick S, Bai W, Edwards A, Jo H, Wu Y, DiMaio D, DeGrado W. De novo-designed transmembrane proteins bind and regulate a cytokine receptor. Nature Chemical Biology 2024, 20: 751-760. PMID: 38480980, PMCID: PMC11142920, DOI: 10.1038/s41589-024-01562-z.Peer-Reviewed Original ResearchTM proteinsTM regionTarget membrane proteinsComplex biological functionsTM domainTM helicesInteraction partnersTransmembrane proteinsMembrane proteinsReceptor homodimerizationBiological functionsCytokine receptorsBinding topologyAmino acidsProteinErythropoietin receptorHomodimerizationCell proliferationTransmembraneEpoRIn vitroMolecular modelingBinding modeReceptorsMotifCorrection for Rasmussen et al., “Virology—the path forward”
Rasmussen A, Gronvall G, Lowen A, Goodrum F, Alwine J, Andersen K, Anthony S, Baines J, Banerjee A, Broadbent A, Brooke C, Campos S, Caposio P, Casadevall A, Chan G, Cliffe A, Collins-McMillen D, Connell N, Damania B, Daugherty M, Debbink K, Dermody T, DiMaio D, Duprex W, Emerman M, Galloway D, Garry R, Goldstein S, Greninger A, Hartman A, Hogue B, Horner S, Hotez P, Jung J, Kamil J, Karst S, Laimins L, Lakdawala S, Landais I, Letko M, Lindenbach B, Liu S, Luftig M, McFadden G, Mehle A, Morrison J, Moscona A, Mühlberger E, Munger J, Münger K, Murphy E, Neufeldt C, Nikolich J, O'Connor C, Pekosz A, Permar S, Pfeiffer J, Popescu S, Purdy J, Racaniello V, Rice C, Runstadler J, Sapp M, Scott R, Smith G, Sorrell E, Speranza E, Streblow D, Tibbetts S, Toth Z, Van Doorslaer K, Weiss S, White E, White T, Wobus C, Worobey M, Yamaoka S, Yurochko A. Correction for Rasmussen et al., “Virology—the path forward”. Journal Of Virology 2024, 98: e00074-24. PMID: 38334328, PMCID: PMC10949460, DOI: 10.1128/jvi.00074-24.Peer-Reviewed Original ResearchT cell receptor inhibition with conditional transmembrane peptide
Ye Y, Morita S, Chang J, Buckley P, Wilhelm K, DiMaio D, Groves J, Barrera F. T cell receptor inhibition with conditional transmembrane peptide. Biophysical Journal 2024, 123: 510a. DOI: 10.1016/j.bpj.2023.11.3079.Peer-Reviewed Original ResearchVirology—the path forward
Rasmussen A, Gronvall G, Lowen A, Goodrum F, Alwine J, Andersen K, Anthony S, Baines J, Banerjee A, Broadbent A, Brooke C, Campos S, Caposio P, Casadevall A, Chan G, Cliffe A, Collins-McMillen D, Connell N, Damania B, Daugherty M, Debbink K, Dermody T, DiMaio D, Duprex W, Emerman M, Galloway D, Garry R, Goldstein S, Greninger A, Hartman A, Hogue B, Horner S, Hotez P, Jung J, Kamil J, Karst S, Laimins L, Lakdawala S, Landais I, Letko M, Lindenbach B, Liu S, Luftig M, McFadden G, Mehle A, Morrison J, Moscona A, Mühlberger E, Munger J, Münger K, Murphy E, Neufeldt C, Nikolich J, O'Connor C, Pekosz A, Permar S, Pfeiffer J, Popescu S, Purdy J, Racaniello V, Rice C, Runstadler J, Sapp M, Scott R, Smith G, Sorrell E, Speranza E, Streblow D, Tibbetts S, Toth Z, Van Doorslaer K, Weiss S, White E, White T, Wobus C, Worobey M, Yamaoka S, Yurochko A. Virology—the path forward. Journal Of Virology 2024, 98: e01791-23. PMID: 38168672, PMCID: PMC10804978, DOI: 10.1128/jvi.01791-23.Peer-Reviewed Original Research
2023
Sequence-independent activity of a predicted long disordered segment of the human papillomavirus type 16 L2 capsid protein during virus entry
Oh C, Buckley P, Choi J, Hierro A, DiMaio D. Sequence-independent activity of a predicted long disordered segment of the human papillomavirus type 16 L2 capsid protein during virus entry. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2307721120. PMID: 37819982, PMCID: PMC10589650, DOI: 10.1073/pnas.2307721120.Peer-Reviewed Original ResearchConceptsAmino acid sequenceAcid sequenceProtein segmentsVirus traffickingUnrelated cellular proteinsSequence-independent mannerIntracellular virus traffickingActivity of proteinsAmino acid segmentComplex biological functionsVirus entryTandem arraysProtein functionTrafficking factorsCellular proteinsEndosome membraneBiological functionsHPV16 pseudovirus infectionCellular factorsDiverse sequencesL2 capsid proteinsAllosteric inhibition of the T cell receptor by a designed membrane ligand
Ye Y, Morita S, Chang J, Buckley P, Wilhelm K, DiMaio D, Groves J, Barrera F. Allosteric inhibition of the T cell receptor by a designed membrane ligand. ELife 2023, 12: e82861. PMID: 37796108, PMCID: PMC10554751, DOI: 10.7554/elife.82861.Peer-Reviewed Original ResearchConceptsT cell receptorTCR activationCD3ζ subunitsTM ligandsComplex molecular machinesReceptor tyrosine kinase EphA2Cell receptorTM domainTransmembrane domainNative nanodiscsTCR subunitsAllosteric changesTM bundleCytoplasmic sideTM regionMolecular mechanismsExtracellular domainAllosteric inhibitionLck phosphorylationMolecular machinesMembrane receptorsAberrant activationSubunitsMembrane ligandsDecades of investigationAnticipating the Next Ten Years of the Annual Review of Virology
Pfeiffer J, Enquist L, DiMaio D, Dermody T. Anticipating the Next Ten Years of the Annual Review of Virology. Annual Review Of Virology 2023, 10: iv-vii. DOI: 10.1146/annurev-vi-10-062723-101111.Peer-Reviewed Original ResearchNoncanonical Rab9a action supports retromer-mediated endosomal exit of human papillomavirus during virus entry
Choi J, DiMaio D. Noncanonical Rab9a action supports retromer-mediated endosomal exit of human papillomavirus during virus entry. PLOS Pathogens 2023, 19: e1011648. PMID: 37703297, PMCID: PMC10519607, DOI: 10.1371/journal.ppat.1011648.Peer-Reviewed Original ResearchConceptsRetromer-mediated endosomeHPV entryGTP-bound formDominant negative Rab7Intracellular vesicular transportRetrograde transport pathwayVirus entryEndosomal exitRab GTPasesRab proteinsVesicle traffickingGolgi transportCellular proteinsVesicular transportProtein cargoKnockdown cellsIntracellular traffickingRab9AIncoming virusRab7EndosomesTraffickingTransport pathwaysProteinKey roleVirology under the Microscope—a Call for Rational Discourse
Goodrum F, Lowen A, Lakdawala S, Alwine J, Casadevall A, Imperiale M, Atwood W, Avgousti D, Baines J, Banfield B, Banks L, Bhaduri-McIntosh S, Bhattacharya D, Blanco-Melo D, Bloom D, Boon A, Boulant S, Brandt C, Broadbent A, Brooke C, Cameron C, Campos S, Caposio P, Chan G, Cliffe A, Coffin J, Collins K, Damania B, Daugherty M, Debbink K, DeCaprio J, Dermody T, Dikeakos J, DiMaio D, Dinglasan R, Duprex W, Dutch R, Elde N, Emerman M, Enquist L, Fane B, Fernandez-Sesma A, Flenniken M, Frappier L, Frieman M, Frueh K, Gack M, Gaglia M, Gallagher T, Galloway D, García-Sastre A, Geballe A, Glaunsinger B, Goff S, Greninger A, Hancock M, Harris E, Heaton N, Heise M, Heldwein E, Hogue B, Horner S, Hutchinson E, Hyser J, Jackson W, Kalejta R, Kamil J, Karst S, Kirchhoff F, Knipe D, Kowalik T, Lagunoff M, Laimins L, Langlois R, Lauring A, Lee B, Leib D, Liu S, Longnecker R, Lopez C, Luftig M, Lund J, Manicassamy B, McFadden G, McIntosh M, Mehle A, Miller W, Mohr I, Moody C, Moorman N, Moscona A, Mounce B, Munger J, Münger K, Murphy E, Naghavi M, Nelson J, Neufeldt C, Nikolich J, O'Connor C, Ono A, Orenstein W, Ornelles D, Ou J, Parker J, Parrish C, Pekosz A, Pellett P, Pfeiffer J, Plemper R, Polyak S, Purdy J, Pyeon D, Quinones-Mateu M, Renne R, Rice C, Schoggins J, Roller R, Russell C, Sandri-Goldin R, Sapp M, Schang L, Schmid S, Schultz-Cherry S, Semler B, Shenk T, Silvestri G, Simon V, Smith G, Smith J, Spindler K, Stanifer M, Subbarao K, Sundquist W, Suthar M, Sutton T, Tai A, Tarakanova V, tenOever B, Tibbetts S, Tompkins S, Toth Z, van Doorslaer K, Vignuzzi M, Wallace N, Walsh D, Weekes M, Weinberg J, Weitzman M, Weller S, Whelan S, White E, Williams B, Wobus C, Wong S, Yurochko A. Virology under the Microscope—a Call for Rational Discourse. MBio 2023, 14: e00188-23. PMID: 36700642, PMCID: PMC9973315, DOI: 10.1128/mbio.00188-23.Peer-Reviewed Original ResearchConceptsOrigin of SARS-CoV-2Evidence-based discourseRational discourseVirology researchGain-of-function approachesVirus-related researchPolicy makersField of virologyLegitimate questionsHuman pathogensDiscourseVocal groupRegulatory structureSARS-CoV-2United StatesPublic concernPublic confusionGroup of individualsVirology under the Microscope—a Call for Rational Discourse
Goodrum F, Lowen A, Lakdawala S, Alwine J, Casadevall A, Imperiale M, Atwood W, Avgousti D, Baines J, Banfield B, Banks L, Bhaduri-McIntosh S, Bhattacharya D, Blanco-Melo D, Bloom D, Boon A, Boulant S, Brandt C, Broadbent A, Brooke C, Cameron C, Campos S, Caposio P, Chan G, Cliffe A, Coffin J, Collins K, Damania B, Daugherty M, Debbink K, DeCaprio J, Dermody T, Dikeakos J, DiMaio D, Dinglasan R, Duprex W, Dutch R, Elde N, Emerman M, Enquist L, Fane B, Fernandez-Sesma A, Flenniken M, Frappier L, Frieman M, Frueh K, Gack M, Gaglia M, Gallagher T, Galloway D, García-Sastre A, Geballe A, Glaunsinger B, Goff S, Greninger A, Hancock M, Harris E, Heaton N, Heise M, Heldwein E, Hogue B, Horner S, Hutchinson E, Hyser J, Jackson W, Kalejta R, Kamil J, Karst S, Kirchhoff F, Knipe D, Kowalik T, Lagunoff M, Laimins L, Langlois R, Lauring A, Lee B, Leib D, Liu S, Longnecker R, Lopez C, Luftig M, Lund J, Manicassamy B, McFadden G, McIntosh M, Mehle A, Miller W, Mohr I, Moody C, Moorman N, Moscona A, Mounce B, Munger J, Münger K, Murphy E, Naghavi M, Nelson J, Neufeldt C, Nikolich J, O'Connor C, Ono A, Orenstein W, Ornelles D, Ou J, Parker J, Parrish C, Pekosz A, Pellett P, Pfeiffer J, Plemper R, Polyak S, Purdy J, Pyeon D, Quinones-Mateu M, Renne R, Rice C, Schoggins J, Roller R, Russell C, Sandri-Goldin R, Sapp M, Schang L, Schmid S, Schultz-Cherry S, Semler B, Shenk T, Silvestri G, Simon V, Smith G, Smith J, Spindler K, Stanifer M, Subbarao K, Sundquist W, Suthar M, Sutton T, Tai A, Tarakanova V, tenOever B, Tibbetts S, Tompkins S, Toth Z, van Doorslaer K, Vignuzzi M, Wallace N, Walsh D, Weekes M, Weinberg J, Weitzman M, Weller S, Whelan S, White E, Williams B, Wobus C, Wong S, Yurochko A. Virology under the Microscope—a Call for Rational Discourse. MSphere 2023, 8: e00034-23. PMID: 36700653, PMCID: PMC10117089, DOI: 10.1128/msphere.00034-23.Peer-Reviewed Original ResearchConceptsOrigin of SARS-CoV-2Evidence-based discourseRational discourseVirology researchGain-of-function approachesVirus-related researchPolicy makersField of virologyLegitimate questionsHuman pathogensDiscourseVocal groupRegulatory structureSARS-CoV-2United StatesPublic concernPublic confusionGroup of individualsVirology under the Microscope—a Call for Rational Discourse
Goodrum F, Lowen A, Lakdawala S, Alwine J, Casadevall A, Imperiale M, Atwood W, Avgousti D, Baines J, Banfield B, Banks L, Bhaduri-McIntosh S, Bhattacharya D, Blanco-Melo D, Bloom D, Boon A, Boulant S, Brandt C, Broadbent A, Brooke C, Cameron C, Campos S, Caposio P, Chan G, Cliffe A, Coffin J, Collins K, Damania B, Daugherty M, Debbink K, DeCaprio J, Dermody T, Dikeakos J, DiMaio D, Dinglasan R, Duprex W, Dutch R, Elde N, Emerman M, Enquist L, Fane B, Fernandez-Sesma A, Flenniken M, Frappier L, Frieman M, Frueh K, Gack M, Gaglia M, Gallagher T, Galloway D, García-Sastre A, Geballe A, Glaunsinger B, Goff S, Greninger A, Hancock M, Harris E, Heaton N, Heise M, Heldwein E, Hogue B, Horner S, Hutchinson E, Hyser J, Jackson W, Kalejta R, Kamil J, Karst S, Kirchhoff F, Knipe D, Kowalik T, Lagunoff M, Laimins L, Langlois R, Lauring A, Lee B, Leib D, Liu S, Longnecker R, Lopez C, Luftig M, Lund J, Manicassamy B, McFadden G, McIntosh M, Mehle A, Miller W, Mohr I, Moody C, Moorman N, Moscona A, Mounce B, Munger J, Münger K, Murphy E, Naghavi M, Nelson J, Neufeldt C, Nikolich J, O'Connor C, Ono A, Orenstein W, Ornelles D, Ou J, Parker J, Parrish C, Pekosz A, Pellett P, Pfeiffer J, Plemper R, Polyak S, Purdy J, Pyeon D, Quinones-Mateu M, Renne R, Rice C, Schoggins J, Roller R, Russell C, Sandri-Goldin R, Sapp M, Schang L, Schmid S, Schultz-Cherry S, Semler B, Shenk T, Silvestri G, Simon V, Smith G, Smith J, Spindler K, Stanifer M, Subbarao K, Sundquist W, Suthar M, Sutton T, Tai A, Tarakanova V, tenOever B, Tibbetts S, Tompkins S, Toth Z, van Doorslaer K, Vignuzzi M, Wallace N, Walsh D, Weekes M, Weinberg J, Weitzman M, Weller S, Whelan S, White E, Williams B, Wobus C, Wong S, Yurochko A. Virology under the Microscope—a Call for Rational Discourse. Journal Of Virology 2023, 97: e00089-23. PMID: 36700640, PMCID: PMC9972907, DOI: 10.1128/jvi.00089-23.Peer-Reviewed Original ResearchConceptsOrigin of SARS-CoV-2Evidence-based discourseRational discourseVirology researchGain-of-function approachesVirus-related researchPolicy makersField of virologyLegitimate questionsHuman pathogensDiscourseVocal groupRegulatory structureSARS-CoV-2United StatesPublic concernPublic confusionGroup of individualsHPV is a cargo for the COPI sorting complex during virus entry
Harwood M, Woo T, Takeo Y, DiMaio D, Tsai B. HPV is a cargo for the COPI sorting complex during virus entry. Science Advances 2023, 9: eadc9830. PMID: 36662862, PMCID: PMC9858521, DOI: 10.1126/sciadv.adc9830.Peer-Reviewed Original ResearchConceptsCoat protein complex ITrans-Golgi networkProtein complex IGene knockdown strategyVirus entryCOPI complexProtein complexesCellular fractionationUnbiased proteomicsRetrograde traffickingCytoplasmic segmentGolgi stacksCellular cargoL2 mutantKnockdown strategyGolgi apparatusComplex IIncoming virusCell surfaceGolgiHost factorsCargoComplexesMutantsEndosomes
2022
Introduction: Virology in the Headlines
Enquist L, DiMaio D, Dermody T. Introduction: Virology in the Headlines. Annual Review Of Virology 2022, 9: i-ii. DOI: 10.1146/annurev-vi-09-071222-100001.Peer-Reviewed Original ResearchHuman Papillomavirus L2 Capsid Protein Stabilizes γ-Secretase during Viral Infection
Crite M, DiMaio D. Human Papillomavirus L2 Capsid Protein Stabilizes γ-Secretase during Viral Infection. Viruses 2022, 14: 804. PMID: 35458534, PMCID: PMC9027364, DOI: 10.3390/v14040804.Peer-Reviewed Original ResearchConceptsTM domainΓ-secretaseVirus traffickingCellular transmembrane proteinsNon-canonical rolePutative TM domainRetrograde transport pathwayΓ-secretase complexSubstrate proteinsMinor capsid protein L2Transmembrane proteinCatalytic subunitMutational studiesEndosomal membranesIntracellular traffickingProtein L2Cellular proteasesCellular factorsL2 capsid proteinsTM mutantsCapsid proteinHPV entryTraffickingL2 proteinProtein
2021
Introduction
Enquist L, Dermody T, DiMaio D. Introduction. Annual Review Of Virology 2021, 8: 1-2. PMID: 34586872, DOI: 10.1146/annurev-vi-08-072621-100001.Peer-Reviewed Original ResearchRetromer stabilizes transient membrane insertion of L2 capsid protein during retrograde entry of human papillomavirus
Xie J, Zhang P, Crite M, Lindsay CV, DiMaio D. Retromer stabilizes transient membrane insertion of L2 capsid protein during retrograde entry of human papillomavirus. Science Advances 2021, 7: eabh4276. PMID: 34193420, PMCID: PMC11057781, DOI: 10.1126/sciadv.abh4276.Peer-Reviewed Original ResearchCell-penetrating peptidesMembrane insertionL2 capsid proteinsArtificial transmembrane proteinsCapsid proteinComplex genetic interactionsTrafficking complexTrafficking stepsGenetic interactionsTraptamersTransmembrane proteinEndosome membraneRetrograde pathwayRetromerHPV entryIncoming virusProteinTransient insertionVirus entryCoherent pathwaysEntry routePathwaySequential passageDifferent stepsUbiquitinationRecurring Revolutions in Virology
Enquist L, DiMaio D, Dermody T. Recurring Revolutions in Virology. Annual Review Of Virology 2021, 8: 1-3. PMID: 33826854, DOI: 10.1146/annurev-vi-08-032921-100002.Peer-Reviewed Original Research
2020
Vaccine Safety, Efficacy, and Trust Take Time
Dermody T, DiMaio D, Enquist L. Vaccine Safety, Efficacy, and Trust Take Time. Annual Review Of Virology 2020, 8: 1-3. PMID: 33186082, DOI: 10.1146/annurev-vi-08-102220-100001.Peer-Reviewed Original Research