2015
Commentary on “Caged Phosphate and the Slips and Misses in Determination of Quantum Yields for Ultraviolet‐A‐Induced Photouncaging” by G. Gasser and Co‐Workers
Corrie JE, Kaplan JH, Forbush B, Ogden DC, Trentham DR. Commentary on “Caged Phosphate and the Slips and Misses in Determination of Quantum Yields for Ultraviolet‐A‐Induced Photouncaging” by G. Gasser and Co‐Workers. ChemPhysChem 2015, 16: 1861-1862. PMID: 25704071, DOI: 10.1002/cphc.201402808.Peer-Reviewed Original Research
2002
Activation of the Na-K-Cl Cotransporter NKCC1 Detected with a Phospho-specific Antibody*
Flemmer AW, Giménez I, Dowd BF, Darman RB, Forbush B. Activation of the Na-K-Cl Cotransporter NKCC1 Detected with a Phospho-specific Antibody*. Journal Of Biological Chemistry 2002, 277: 37551-37558. PMID: 12145305, DOI: 10.1074/jbc.m206294200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibody SpecificityBinding SitesColonDipeptidesEpinephrineHumansIon TransportIsoproterenolKineticsMolecular Sequence DataMutagenesis, Site-DirectedPhosphatesPhosphopeptidesRatsRecombinant ProteinsSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2ThreonineTracheaConceptsPhospho-specific antibodiesNa-K-Cl cotransporter NKCC1Divergent vertebrate speciesBasolateral membraneShark rectal glandPhosphorylation of NKCC1Regulatory lociHEK-293 cellsHigh conservationThreonine residuesPhosphorylation experimentsVertebrate speciesRegulatory domainCotransporter NKCC1NKCC1 regulationN-terminusRectal glandCell membranePhosphorylationImmunofluorescence analysisAgonist stimulationActivation stateGland tubulesRectal gland tubulesEpithelial cells
1994
Kinetic heterogeneity of phosphoenzyme of Na,K-ATPase modeled by unmixed lipid phases. Competence of the phosphointermediate.
Klodos I, Post R, Forbush B. Kinetic heterogeneity of phosphoenzyme of Na,K-ATPase modeled by unmixed lipid phases. Competence of the phosphointermediate. Journal Of Biological Chemistry 1994, 269: 1734-1743. PMID: 8294422, DOI: 10.1016/s0021-9258(17)42089-8.Peer-Reviewed Original Research
1992
The Na-K-Cl cotransporter of avian salt gland. Phosphorylation in response to cAMP-dependent and calcium-dependent secretogogues.
Torchia J, Lytle C, Pon D, Forbush B, Sen A. The Na-K-Cl cotransporter of avian salt gland. Phosphorylation in response to cAMP-dependent and calcium-dependent secretogogues. Journal Of Biological Chemistry 1992, 267: 25444-25450. PMID: 1281159, DOI: 10.1016/s0021-9258(19)74061-7.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine8-Bromo Cyclic Adenosine MonophosphateAnimalsBlotting, WesternCalciumCarbacholCarrier ProteinsCell MembraneColforsinCyclic AMPDucksKineticsMembrane ProteinsPhosphatesPhosphorylationSalt GlandSodium-Potassium-Chloride SymportersTime FactorsVasoactive Intestinal PeptideConceptsNa-K-Cl cotransporterPhosphorylation of pp170Avian salt glandMonoclonal antibodiesResponse to carbacholEffect of forskolinNa-K-ClIntact cell preparationsConcentration-dependent increaseStimulation of secretionIncreased approximately 5-foldCAMP accumulationExposure of cellsCAMP-dependent protein kinasePp170Approximately 5-foldCell preparationsCAMP-dependentSalt gland cellsGland membranesWestern blottingForskolinSecretogoguesVIPAntibodies
1988
Rapid release of 45Ca from an occluded state of the Na,K-pump.
Forbush B. Rapid release of 45Ca from an occluded state of the Na,K-pump. Journal Of Biological Chemistry 1988, 263: 7970-7978. PMID: 2836404, DOI: 10.1016/s0021-9258(18)68429-7.Peer-Reviewed Original ResearchConceptsNa,K-pumpNa,K-ATPaseK-pumpK-ATPasePhosphorylation of Na,K-ATPaseRelease of 45CaExposure to K+Release of 86RbApparent affinityCa2+Transport sitesK+ congenerMg2+ + ATPIntracellular faceRelease of K+Simultaneous occlusionExtracellular sitesPrevent phosphorylationCa2Exposure to Mg2Extracellular faceReleaseAbsence of PiN-methylglucamineIntracellular mediumThe interaction of amines with the occluded state of the Na,K-pump.
Forbush B. The interaction of amines with the occluded state of the Na,K-pump. Journal Of Biological Chemistry 1988, 263: 7979-7988. PMID: 2836405, DOI: 10.1016/s0021-9258(18)68430-3.Peer-Reviewed Original ResearchConceptsRelease of 86RbInteraction of aminesPresence of aminesNa,K-pumpIntracellular faceDog kidney NaK-pumpK-ATPaseEffect of aminesBenzyl amineBifunctional aminesBlock releaseKidney NaOcclusionOrganic cationsPre-incubationAminesAmine blocksTransport sitesPresence of ATPK+ siteCompetitive mannerLow affinityOccluded state
1987
Rapid release of 42K or 86Rb from two distinct transport sites on the Na,K-pump in the presence of Pi or vanadate.
Forbush B. Rapid release of 42K or 86Rb from two distinct transport sites on the Na,K-pump in the presence of Pi or vanadate. Journal Of Biological Chemistry 1987, 262: 11116-11127. PMID: 2440884, DOI: 10.1016/s0021-9258(18)60933-0.Peer-Reviewed Original Research
1979
Evidence that ouabain binds to the same large polypeptide chain of dimeric Na,K-ATPase that is phosphorylated from Pi.
Forbush B, Hoffman J. Evidence that ouabain binds to the same large polypeptide chain of dimeric Na,K-ATPase that is phosphorylated from Pi. Biochemistry 1979, 18: 2308-15. PMID: 221003, DOI: 10.1021/bi00578a027.Peer-Reviewed Original Research