2022
A molecular beacon assay for monitoring RNA splicing
Omran QQ, Fedorova O, Liu T, Pyle AM. A molecular beacon assay for monitoring RNA splicing. Nucleic Acids Research 2022, 50: e74-e74. PMID: 35438748, PMCID: PMC9303364, DOI: 10.1093/nar/gkac242.Peer-Reviewed Original ResearchConceptsMolecular beaconsMolecular beacon assayHigher fluorescent signalHigh-throughput screening platformSmall moleculesAutocatalyzed reactionMolecular beacon approachKinetic characterizationReactionSmall molecule targetingInhibition constantsScreening platformSmall molecule inhibitorsFluorescent signalMolecule targetingMolecule inhibitorsMolecules
2019
RIG-I Recognition of RNA Targets: The Influence of Terminal Base Pair Sequence and Overhangs on Affinity and Signaling
Ren X, Linehan MM, Iwasaki A, Pyle AM. RIG-I Recognition of RNA Targets: The Influence of Terminal Base Pair Sequence and Overhangs on Affinity and Signaling. Cell Reports 2019, 29: 3807-3815.e3. PMID: 31851914, DOI: 10.1016/j.celrep.2019.11.052.Peer-Reviewed Original ResearchConceptsRNA moleculesRIG-I activationBase pair sequenceHost RNA moleculesViral RNA moleculesRIG-I recognitionMolecular basisRNA variantsRNA targetsPair sequenceHuman cellsBase pairsImmune receptorsMechanisms of evasionTerminal base pairsLigand affinityWhole animalInterferon responseDeadly pathogenRNA therapeuticsMarburg virusCellsOverhangMoleculesSignaling
2000
[10] Using DNAzylnes to cut, process, and map RNA molecules for structural studies or modification
Pyle AM, Chu VT, Jankowsky E, Boudvillain M. [10] Using DNAzylnes to cut, process, and map RNA molecules for structural studies or modification. Methods In Enzymology 2000, 317: 140-146. PMID: 10829278, DOI: 10.1016/s0076-6879(00)17012-0.Peer-Reviewed Original ResearchConceptsStructural studiesTypes of DNAzymeTemplate-directed ligationDNAzyme reactionDNAzyme moleculesSimple DNA moleculesDNAzymeDivalent ionsAnalytical scaleMoleculesDNA moleculesAddition of Mg2Crystallographic applicationsRNA targetsRNA moleculesCatalytic coreIonsLong RNAsReactionModificationDifferent applicationsInexpensive wayRNA fragmentsMg2Incorporation