2021
Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis
Santoro F, Chien K, Sahara M. Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis. STAR Protocols 2021, 2: 100339. PMID: 33644774, PMCID: PMC7887647, DOI: 10.1016/j.xpro.2021.100339.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cell differentiationEmbryonic stem cell differentiationSingle-cell RNA-seq analysisSingle-cell RNA sequencing analysisComprehensive transcriptional analysisRNA-seq analysisStem cell differentiationRNA sequencing analysisCardiac derivativesFluorescence-activated cell sortingSmart-seq2Developmental tissuesTranscriptional analysisCombination of populationCDNA libraryMolecular atlasHuman embryogenesisHuman ESCsCell differentiationSequencing analysisComplete detailsCell sortingPowerful approachEmbryonic heart cellsDifferentiation
2019
Cardiac progenitors and paracrine mediators in cardiogenesis and heart regeneration
Witman N, Zhou C, Grote Beverborg N, Sahara M, Chien K. Cardiac progenitors and paracrine mediators in cardiogenesis and heart regeneration. Seminars In Cell And Developmental Biology 2019, 100: 29-51. PMID: 31862220, DOI: 10.1016/j.semcdb.2019.10.011.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCardiac progenitorsHeart regenerationCardiac progenitor cell biologyMammalian cardiac developmentProgenitor cell biologyParacrine mediatorsMorphogenetic processesCell biologyCardiac developmentMolecular mechanismsCell-free therapyCardiogenesisModern biotechnologyDiseased settingsMammalian heartProgenitorsCongenital heart diseaseRegenerative capabilityTissue engineering platformsRecent knowledgeEngineering platformHeart diseaseNovel toolHeart repairRegenerationPopulation and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract
Sahara M, Santoro F, Sohlmér J, Zhou C, Witman N, Leung CY, Mononen M, Bylund K, Gruber P, Chien KR. Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract. Developmental Cell 2019, 48: 475-490.e7. PMID: 30713072, DOI: 10.1016/j.devcel.2019.01.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineCells, CulturedEmbryonic Stem CellsEndothelial CellsHeart VentriclesHuman Embryonic Stem CellsHumansLIM-Homeodomain ProteinsMice, Inbred C57BLMultipotent Stem CellsMyocardiumMyocytes, CardiacOrganogenesisReceptors, G-Protein-CoupledSingle-Cell AnalysisConceptsCardiac stem/progenitor cellsMultipotent cardiac stem/progenitor cellsCardiac developmentMammalian cardiac developmentSingle-cell RNA-seqComprehensive gene expression profilesGene expression profilesHuman embryonic stemSingle-cell analysisStem/progenitor cellsMammalian cardiogenesisHuman cardiogenesisRNA-seqMorphogenetic processesProximal outflow tractEmbryonic stemEmbryonic outflow tractExpression profilesVentricular progenitorsPutative originHuman embryonic heartCardiac cellsEmbryonic heartProgenitor cellsCardiac derivatives
2013
Driving vascular endothelial cell fate of human multipotent Isl1+ heart progenitors with VEGF modified mRNA
Lui K, Zangi L, Silva E, Bu L, Sahara M, Li R, Mooney D, Chien K. Driving vascular endothelial cell fate of human multipotent Isl1+ heart progenitors with VEGF modified mRNA. Cell Research 2013, 23: 1172-1186. PMID: 24018375, PMCID: PMC3790234, DOI: 10.1038/cr.2013.112.Peer-Reviewed Original ResearchConceptsEndothelial specificationHeart progenitorsEndothelial cell fateCell fate decisionsCell fate switchHuman embryonic stem cellsCell-specific genesStem cellsEmbryonic stem cellsHuman pluripotent stem cellsEndothelial cell lineagesPluripotent stem cellsLarge-scale derivationCell fateMammalian cardiogenesisCardiovascular progenitorsMultipotent progenitorsHuman ESCsRegenerative therapeutic potentialCell lineagesEfficient expressionDistinct familiesAngiocrine factorsIsl1Endothelial intermediateA HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells
Später D, Abramczuk M, Buac K, Zangi L, Stachel M, Clarke J, Sahara M, Ludwig A, Chien K. A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells. Nature Cell Biology 2013, 15: 1098-1106. PMID: 23974038, DOI: 10.1038/ncb2824.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCell DifferentiationCell LineageCyclic Nucleotide-Gated Cation ChannelsEmbryo, MammalianEmbryonic Stem CellsGene Expression Regulation, DevelopmentalHeart AtriaHeart VentriclesHumansHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMesodermMiceMorphogenesisMuscle ProteinsMyocardiumMyocytes, CardiacPluripotent Stem CellsPotassium ChannelsConceptsHeart fieldFirst heart fieldHuman embryonic stem cellsSecond heart fieldStem cellsEmbryonic stem cellsHuman pluripotent stem cellsPluripotent stem cellsMesodermal cellsDifferentiation culturesCardiomyogenic lineageCardiomyogenic progenitorsMammalian heartProgenitorsLineagesDistinct groupsHCN4CellsMarkers