2022
Progress in Bioengineering Strategies for Heart Regenerative Medicine
Häneke T, Sahara M. Progress in Bioengineering Strategies for Heart Regenerative Medicine. International Journal Of Molecular Sciences 2022, 23: 3482. PMID: 35408844, PMCID: PMC8998628, DOI: 10.3390/ijms23073482.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsBioengineeringHeart DiseasesHumansMyocytes, CardiacPluripotent Stem CellsRegenerative MedicineTissue EngineeringConceptsHuman pluripotent stem cellsCardiac tissue engineering strategiesRegenerative medicineStem cell engineeringHeart regenerationNovel functional biomaterialsCardiac tissue engineeringStem cell biologyTissue engineering strategiesHeart diseasePluripotent stem cellsEngineering strategiesCell engineeringFunctional biomaterialsTissue engineeringCardiac biologyCell biologyBioengineering strategiesGreat promiseCardiomyocyte maturationDisease modelingHeart regenerative medicineInsufficient therapeutic optionsStem cellsTherapeutic applicationsUncovering the molecular identity of cardiosphere-derived cells (CDCs) by single-cell RNA sequencing
Kogan P, Wirth F, Tomar A, Darr J, Teperino R, Lahm H, Dreßen M, Puluca N, Zhang Z, Neb I, Beck N, Luzius T, de la Osa de la Rosa L, Gärtner K, Hüls C, Zeidler R, Ramanujam D, Engelhardt S, Wenk C, Holdt L, Mononen M, Sahara M, Cleuziou J, Hörer J, Lange R, Krane M, Doppler S. Uncovering the molecular identity of cardiosphere-derived cells (CDCs) by single-cell RNA sequencing. Basic Research In Cardiology 2022, 117: 11. PMID: 35258704, PMCID: PMC8902493, DOI: 10.1007/s00395-022-00913-y.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingExtracellular vesiclesRNA sequencingMolecular identityCell typesMitochondria-rich cell typesCardiosphere-derived cellsMitochondria-rich cellsHuman-induced pluripotent stem cellsPluripotent stem cellsCardiac progenitor cellsPro-apoptotic BaxGO termsNon-hematopoietic cellsCardiac developmentTranscriptomic similarityStem cellsProgenitor cellsCellular originNon-myocyte cellsNew specific markerCulture conditionsBiological similaritiesSpecial culture conditionsSequencing
2019
Cardiac progenitors and paracrine mediators in cardiogenesis and heart regeneration
Witman N, Zhou C, Grote Beverborg N, Sahara M, Chien K. Cardiac progenitors and paracrine mediators in cardiogenesis and heart regeneration. Seminars In Cell And Developmental Biology 2019, 100: 29-51. PMID: 31862220, DOI: 10.1016/j.semcdb.2019.10.011.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsAnimalsHumansMyocardiumMyocytes, CardiacParacrine CommunicationPluripotent Stem CellsRegenerationTissue EngineeringConceptsCardiac progenitorsHeart regenerationCardiac progenitor cell biologyMammalian cardiac developmentProgenitor cell biologyParacrine mediatorsMorphogenetic processesCell biologyCardiac developmentMolecular mechanismsCell-free therapyCardiogenesisModern biotechnologyDiseased settingsMammalian heartProgenitorsCongenital heart diseaseRegenerative capabilityTissue engineering platformsRecent knowledgeEngineering platformHeart diseaseNovel toolHeart repairRegenerationPopulation and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract
Sahara M, Santoro F, Sohlmér J, Zhou C, Witman N, Leung CY, Mononen M, Bylund K, Gruber P, Chien KR. Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract. Developmental Cell 2019, 48: 475-490.e7. PMID: 30713072, DOI: 10.1016/j.devcel.2019.01.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineCells, CulturedEmbryonic Stem CellsEndothelial CellsHeart VentriclesHuman Embryonic Stem CellsHumansLIM-Homeodomain ProteinsMice, Inbred C57BLMultipotent Stem CellsMyocardiumMyocytes, CardiacOrganogenesisReceptors, G-Protein-CoupledSingle-Cell AnalysisConceptsCardiac stem/progenitor cellsMultipotent cardiac stem/progenitor cellsCardiac developmentMammalian cardiac developmentSingle-cell RNA-seqComprehensive gene expression profilesGene expression profilesHuman embryonic stemSingle-cell analysisStem/progenitor cellsMammalian cardiogenesisHuman cardiogenesisRNA-seqMorphogenetic processesProximal outflow tractEmbryonic stemEmbryonic outflow tractExpression profilesVentricular progenitorsPutative originHuman embryonic heartCardiac cellsEmbryonic heartProgenitor cellsCardiac derivatives
2015
Programming and reprogramming a human heart cell
Sahara M, Santoro F, Chien K. Programming and reprogramming a human heart cell. The EMBO Journal 2015, 34: 710-738. PMID: 25712211, PMCID: PMC4369310, DOI: 10.15252/embj.201490563.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsStem cell biologyCardiac regenerative therapeuticsHuman heart cellsCardiac regenerative medicineHuman cardiogenesisMolecular programsCell biologyRegenerative therapeuticsCardiac regenerationHeart cellsRegenerative medicineDevelopmental cardiologyLatest discoveriesNovel therapeutic toolDiseased heartCardiac muscleCellsCardiogenesisTherapeutic strategiesClinical practiceTherapeutic toolBiologyModest outcomesCurrent controversiesDeeper understanding
2013
A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells
Später D, Abramczuk M, Buac K, Zangi L, Stachel M, Clarke J, Sahara M, Ludwig A, Chien K. A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells. Nature Cell Biology 2013, 15: 1098-1106. PMID: 23974038, DOI: 10.1038/ncb2824.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCell DifferentiationCell LineageCyclic Nucleotide-Gated Cation ChannelsEmbryo, MammalianEmbryonic Stem CellsGene Expression Regulation, DevelopmentalHeart AtriaHeart VentriclesHumansHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMesodermMiceMorphogenesisMuscle ProteinsMyocardiumMyocytes, CardiacPluripotent Stem CellsPotassium ChannelsConceptsHeart fieldFirst heart fieldHuman embryonic stem cellsSecond heart fieldStem cellsEmbryonic stem cellsHuman pluripotent stem cellsPluripotent stem cellsMesodermal cellsDifferentiation culturesCardiomyogenic lineageCardiomyogenic progenitorsMammalian heartProgenitorsLineagesDistinct groupsHCN4CellsMarkers
2011
The ATP-Binding Cassette Transporter ABCG2 Protects Against Pressure Overload–Induced Cardiac Hypertrophy and Heart Failure by Promoting Angiogenesis and Antioxidant Response
Higashikuni Y, Sainz J, Nakamura K, Takaoka M, Enomoto S, Iwata H, Tanaka K, Sahara M, Hirata Y, Nagai R, Sata M. The ATP-Binding Cassette Transporter ABCG2 Protects Against Pressure Overload–Induced Cardiac Hypertrophy and Heart Failure by Promoting Angiogenesis and Antioxidant Response. Arteriosclerosis Thrombosis And Vascular Biology 2011, 32: 654-661. PMID: 22116099, DOI: 10.1161/atvbaha.111.240341.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAntioxidantsATP Binding Cassette Transporter, Subfamily G, Member 2ATP-Binding Cassette TransportersCells, CulturedDisease Models, AnimalEndothelial CellsGenotypeGlutathioneHeart FailureHindlimbHumansHypertrophy, Left VentricularIschemiaMaleMiceMice, KnockoutMuscle, SkeletalMyocytes, CardiacNeoplasm ProteinsNeovascularization, PhysiologicOxidative StressPhenotypeRatsRats, WistarRNA InterferenceTime FactorsTransfectionVentricular FunctionVentricular RemodelingConceptsTransverse aortic constrictionWild-type micePressure overload-induced cardiac hypertrophyMicrovascular endothelial cellsOverload-induced cardiac hypertrophyCardiac hypertrophyHeart failureEndothelial cellsCassette transporter subfamily G member 2Exaggerated cardiac hypertrophyAntioxidant responseG member 2Tissue defense mechanismsSuperoxide dismutase mimeticCassette transporter ABCG2Cardiac dysfunctionImportant endogenous antioxidantPressure overloadVentricular remodelingAortic constrictionFunctional impairmentATP-Binding Cassette Transporter ABCG2Cardiomyocyte hypertrophyImpaired angiogenesisDismutase mimetic