2022
Progress in Bioengineering Strategies for Heart Regenerative Medicine
Häneke T, Sahara M. Progress in Bioengineering Strategies for Heart Regenerative Medicine. International Journal Of Molecular Sciences 2022, 23: 3482. PMID: 35408844, PMCID: PMC8998628, DOI: 10.3390/ijms23073482.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHuman pluripotent stem cellsCardiac tissue engineering strategiesRegenerative medicineStem cell engineeringHeart regenerationNovel functional biomaterialsCardiac tissue engineeringStem cell biologyTissue engineering strategiesHeart diseasePluripotent stem cellsEngineering strategiesCell engineeringFunctional biomaterialsTissue engineeringCardiac biologyCell biologyBioengineering strategiesGreat promiseCardiomyocyte maturationDisease modelingHeart regenerative medicineInsufficient therapeutic optionsStem cellsTherapeutic applicationsUncovering the molecular identity of cardiosphere-derived cells (CDCs) by single-cell RNA sequencing
Kogan P, Wirth F, Tomar A, Darr J, Teperino R, Lahm H, Dreßen M, Puluca N, Zhang Z, Neb I, Beck N, Luzius T, de la Osa de la Rosa L, Gärtner K, Hüls C, Zeidler R, Ramanujam D, Engelhardt S, Wenk C, Holdt L, Mononen M, Sahara M, Cleuziou J, Hörer J, Lange R, Krane M, Doppler S. Uncovering the molecular identity of cardiosphere-derived cells (CDCs) by single-cell RNA sequencing. Basic Research In Cardiology 2022, 117: 11. PMID: 35258704, PMCID: PMC8902493, DOI: 10.1007/s00395-022-00913-y.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingExtracellular vesiclesRNA sequencingMolecular identityCell typesMitochondria-rich cell typesCardiosphere-derived cellsMitochondria-rich cellsHuman-induced pluripotent stem cellsPluripotent stem cellsCardiac progenitor cellsPro-apoptotic BaxGO termsNon-hematopoietic cellsCardiac developmentTranscriptomic similarityStem cellsProgenitor cellsCellular originNon-myocyte cellsNew specific markerCulture conditionsBiological similaritiesSpecial culture conditionsSequencing
2015
Erratum: Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells
Sahara M, Hansson E, Wernet O, Lui K, Später D, Chien K. Erratum: Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells. Cell Research 2015, 25: 148-148. PMID: 25553820, PMCID: PMC4650595, DOI: 10.1038/cr.2015.2.Commentaries, Editorials and Letters
2014
Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells
Sahara M, Hansson E, Wernet O, Lui K, Später D, Chien K. Manipulation of a VEGF-Notch signaling circuit drives formation of functional vascular endothelial progenitors from human pluripotent stem cells. Cell Research 2014, 24: 820-841. PMID: 24810299, PMCID: PMC4085760, DOI: 10.1038/cr.2014.59.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBone Morphogenetic Protein 4CadherinsCell DifferentiationCell LineEmbryonic Stem CellsEndothelial CellsEndothelium, VascularGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHumansMicePluripotent Stem CellsReceptors, NotchSignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsHuman pluripotent stem cellsPluripotent stem cellsEndothelial lineage cellsEndothelial progenitorsLineage cellsStem cellsVascular endothelial progenitorsVE-cadherin promoterEndothelial cellsGreen fluorescent protein expressionFluorescent protein expressionBioactive small moleculesFunctional vessel networksMesodermal precursorsReporter cell lineDrive formationEndothelial lineageGSK-3β inhibitorDifferentiation protocolsMature endothelial cellsAttractive cell populationRapid large-scale productionEfficient differentiationEndothelial differentiationPharmaceutical inhibition
2013
Driving vascular endothelial cell fate of human multipotent Isl1+ heart progenitors with VEGF modified mRNA
Lui K, Zangi L, Silva E, Bu L, Sahara M, Li R, Mooney D, Chien K. Driving vascular endothelial cell fate of human multipotent Isl1+ heart progenitors with VEGF modified mRNA. Cell Research 2013, 23: 1172-1186. PMID: 24018375, PMCID: PMC3790234, DOI: 10.1038/cr.2013.112.Peer-Reviewed Original ResearchConceptsEndothelial specificationHeart progenitorsEndothelial cell fateCell fate decisionsCell fate switchHuman embryonic stem cellsCell-specific genesStem cellsEmbryonic stem cellsHuman pluripotent stem cellsEndothelial cell lineagesPluripotent stem cellsLarge-scale derivationCell fateMammalian cardiogenesisCardiovascular progenitorsMultipotent progenitorsHuman ESCsRegenerative therapeutic potentialCell lineagesEfficient expressionDistinct familiesAngiocrine factorsIsl1Endothelial intermediateA HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells
Später D, Abramczuk M, Buac K, Zangi L, Stachel M, Clarke J, Sahara M, Ludwig A, Chien K. A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells. Nature Cell Biology 2013, 15: 1098-1106. PMID: 23974038, DOI: 10.1038/ncb2824.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCell DifferentiationCell LineageCyclic Nucleotide-Gated Cation ChannelsEmbryo, MammalianEmbryonic Stem CellsGene Expression Regulation, DevelopmentalHeart AtriaHeart VentriclesHumansHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMesodermMiceMorphogenesisMuscle ProteinsMyocardiumMyocytes, CardiacPluripotent Stem CellsPotassium ChannelsConceptsHeart fieldFirst heart fieldHuman embryonic stem cellsSecond heart fieldStem cellsEmbryonic stem cellsHuman pluripotent stem cellsPluripotent stem cellsMesodermal cellsDifferentiation culturesCardiomyogenic lineageCardiomyogenic progenitorsMammalian heartProgenitorsLineagesDistinct groupsHCN4CellsMarkers
2005
Comparison of Various Bone Marrow Fractions in the Ability to Participate in Vascular Remodeling After Mechanical Injury
Sahara M, Sata M, Matsuzaki Y, Tanaka K, Morita T, Hirata Y, Okano H, Nagai R. Comparison of Various Bone Marrow Fractions in the Ability to Participate in Vascular Remodeling After Mechanical Injury. Stem Cells 2005, 23: 874-878. PMID: 15941860, DOI: 10.1634/stemcells.2005-0012.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsGreen fluorescent proteinGFP-positive cellsBone marrow cellsTotal bone marrow cellsMarrow cellsFluorescent proteinCell typesBone marrow fractionLin- cellsSca-1Stem cellsVascular cellsMechanical injuryMarrow fractionsCellsHSC cellsBroad potentialBone marrow reconstitutionPluripotencyVascular remodelingLesion formationRecent reportsProteinTransdifferentiation