2023
STRA6 is essential for induction of vascular smooth muscle lineages in human embryonic cardiac outflow tract development
Zhou C, Häneke T, Rohner E, Sohlmér J, Kameneva P, Artemov A, Adameyko I, Sahara M. STRA6 is essential for induction of vascular smooth muscle lineages in human embryonic cardiac outflow tract development. Cardiovascular Research 2023, 119: 1202-1217. PMID: 36635482, PMCID: PMC10202647, DOI: 10.1093/cvr/cvad010.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsHuman cardiogenesisWild-type human embryonic stem cellsSmooth muscle cellsCardiac outflow tract developmentSingle-cell RNA sequencing datasetsRARα/RXRαCardiogenic transcription factorsRNA sequencing datasetsSmooth muscle lineageRetinoic acid signalingEmbryonic stem cellsVascular smooth muscle lineageRNA-seq dataOutflow tract developmentCardiac outflow tractOFT formationHeart progenitorsMuscle lineageSTRA6 mutationsRas signalingMurine embryonic heartTranscription factorsAcid signalingHeart development
2013
Driving vascular endothelial cell fate of human multipotent Isl1+ heart progenitors with VEGF modified mRNA
Lui K, Zangi L, Silva E, Bu L, Sahara M, Li R, Mooney D, Chien K. Driving vascular endothelial cell fate of human multipotent Isl1+ heart progenitors with VEGF modified mRNA. Cell Research 2013, 23: 1172-1186. PMID: 24018375, PMCID: PMC3790234, DOI: 10.1038/cr.2013.112.Peer-Reviewed Original ResearchConceptsEndothelial specificationHeart progenitorsEndothelial cell fateCell fate decisionsCell fate switchHuman embryonic stem cellsCell-specific genesStem cellsEmbryonic stem cellsHuman pluripotent stem cellsEndothelial cell lineagesPluripotent stem cellsLarge-scale derivationCell fateMammalian cardiogenesisCardiovascular progenitorsMultipotent progenitorsHuman ESCsRegenerative therapeutic potentialCell lineagesEfficient expressionDistinct familiesAngiocrine factorsIsl1Endothelial intermediateA HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells
Später D, Abramczuk M, Buac K, Zangi L, Stachel M, Clarke J, Sahara M, Ludwig A, Chien K. A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells. Nature Cell Biology 2013, 15: 1098-1106. PMID: 23974038, DOI: 10.1038/ncb2824.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCell DifferentiationCell LineageCyclic Nucleotide-Gated Cation ChannelsEmbryo, MammalianEmbryonic Stem CellsGene Expression Regulation, DevelopmentalHeart AtriaHeart VentriclesHumansHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMesodermMiceMorphogenesisMuscle ProteinsMyocardiumMyocytes, CardiacPluripotent Stem CellsPotassium ChannelsConceptsHeart fieldFirst heart fieldHuman embryonic stem cellsSecond heart fieldStem cellsEmbryonic stem cellsHuman pluripotent stem cellsPluripotent stem cellsMesodermal cellsDifferentiation culturesCardiomyogenic lineageCardiomyogenic progenitorsMammalian heartProgenitorsLineagesDistinct groupsHCN4CellsMarkers