2021
Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis
Santoro F, Chien K, Sahara M. Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis. STAR Protocols 2021, 2: 100339. PMID: 33644774, PMCID: PMC7887647, DOI: 10.1016/j.xpro.2021.100339.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cell differentiationEmbryonic stem cell differentiationSingle-cell RNA-seq analysisSingle-cell RNA sequencing analysisComprehensive transcriptional analysisRNA-seq analysisStem cell differentiationRNA sequencing analysisCardiac derivativesFluorescence-activated cell sortingSmart-seq2Developmental tissuesTranscriptional analysisCombination of populationCDNA libraryMolecular atlasHuman embryogenesisHuman ESCsCell differentiationSequencing analysisComplete detailsCell sortingPowerful approachEmbryonic heart cellsDifferentiation
2019
Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract
Sahara M, Santoro F, Sohlmér J, Zhou C, Witman N, Leung CY, Mononen M, Bylund K, Gruber P, Chien KR. Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract. Developmental Cell 2019, 48: 475-490.e7. PMID: 30713072, DOI: 10.1016/j.devcel.2019.01.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineCells, CulturedEmbryonic Stem CellsEndothelial CellsHeart VentriclesHuman Embryonic Stem CellsHumansLIM-Homeodomain ProteinsMice, Inbred C57BLMultipotent Stem CellsMyocardiumMyocytes, CardiacOrganogenesisReceptors, G-Protein-CoupledSingle-Cell AnalysisConceptsCardiac stem/progenitor cellsMultipotent cardiac stem/progenitor cellsCardiac developmentMammalian cardiac developmentSingle-cell RNA-seqComprehensive gene expression profilesGene expression profilesHuman embryonic stemSingle-cell analysisStem/progenitor cellsMammalian cardiogenesisHuman cardiogenesisRNA-seqMorphogenetic processesProximal outflow tractEmbryonic stemEmbryonic outflow tractExpression profilesVentricular progenitorsPutative originHuman embryonic heartCardiac cellsEmbryonic heartProgenitor cellsCardiac derivatives