2021
Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium
Cai H, Gong J, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium. Experimental Eye Research 2021, 207: 108576. PMID: 33895162, DOI: 10.1016/j.exer.2021.108576.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationRetinal pigment epitheliumMacular degenerationPigment epitheliumInduced pluripotent stem cellsEtiology of AMDMitochondrial dysfunctionAge-matched controlsNovel therapeutic targetTranscriptome of fibroblastsAMD patientsNormal donorsFibroblasts of patientsTherapeutic targetPatientsMore studiesAltered transcriptomeDisease phenotypeSignificant differencesCell linesMitochondrial functionDysfunctionOriginal fibroblastsDistinct transcriptomesDegeneration
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina