1989
A growth hormone-vesicular stomatitis virus G hybrid protein is rapidly degraded in lysosomes following transport to the cell surface.
Rizzolo L. A growth hormone-vesicular stomatitis virus G hybrid protein is rapidly degraded in lysosomes following transport to the cell surface. European Journal Of Cell Biology 1989, 49: 92-8. PMID: 2547615.Peer-Reviewed Original ResearchAnimalsCells, CulturedChloroquineCricetinaeEndocytosisGrowth HormoneKidneyLeupeptinsLysosomesMembrane ProteinsMonensinVesicular stomatitis Indiana virusViral Proteins
1987
Nonpolarized secretion of truncated forms of the influenza hemagglutinin and the vesicular stomatitus virus G protein from MDCK cells.
Gonzalez A, Rizzolo L, Rindler M, Adesnik M, Sabatini D, Gottlieb T. Nonpolarized secretion of truncated forms of the influenza hemagglutinin and the vesicular stomatitus virus G protein from MDCK cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 3738-3742. PMID: 3035552, PMCID: PMC304951, DOI: 10.1073/pnas.84.11.3738.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell Transformation, ViralGenesGenes, ViralGenetic VectorsHemagglutinins, ViralInfluenza A virusMembrane GlycoproteinsMembrane ProteinsVesicular stomatitis Indiana virusViral Envelope ProteinsViral ProteinsConceptsApical plasma membrane domainMDCK cellsPlasma membrane domainsPolarity of secretionVirus G proteinViral glycoproteinsVesicular stomatitus virusMembrane anchoringCorresponding genesMembrane domainsCytoplasmic segmentNonpolarized fashionG proteinsEndogenous glycoproteinsApical surfaceProteinEpithelial cellsGlycoprotein GG glycoproteinGlycoproteinConfluent monolayersCellsInfluenza hemagglutininCDNAGenes
1986
Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells.
Gottlieb T, Gonzalez A, Rizzolo L, Rindler M, Adesnik M, Sabatini D. Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells. Journal Of Cell Biology 1986, 102: 1242-1255. PMID: 3007530, PMCID: PMC2114186, DOI: 10.1083/jcb.102.4.1242.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChloroquineCloning, MolecularDNADogsEndocytosisFluorescent Antibody TechniqueGlycoproteinsKidneyPlasmidsTransfectionVesicular stomatitis Indiana virusViral Envelope ProteinsConceptsG proteinsCell surfaceVesicular stomatitis virusApical plasma membrane domainPlasma membrane domainsPlasma membrane proteinsCell surface domainsApical cell surfaceMadin-Darby canine kidney cellsG protein moleculesViral glycoproteinsLysosome-like vesiclesCanine kidney cellsProtein synthesis inhibitorMembrane domainsMembrane proteinsSurface domainsBasolateral domainEukaryotic expression plasmidTransfection experimentsExpression plasmidProteinPresence of chloroquineApical surfaceProtein molecules
1985
Biosynthesis and intracellular sorting of growth hormone-viral envelope glycoprotein hybrids.
Rizzolo L, Finidori J, Gonzalez A, Arpin M, Ivanov I, Adesnik M, Sabatini D. Biosynthesis and intracellular sorting of growth hormone-viral envelope glycoprotein hybrids. Journal Of Cell Biology 1985, 101: 1351-1362. PMID: 2995406, PMCID: PMC2113904, DOI: 10.1083/jcb.101.4.1351.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCell LineChlorocebus aethiopsDogsEndoplasmic ReticulumFibroblastsGenes, SyntheticGenetic VectorsGlycoproteinsGrowth HormoneHemagglutinins, ViralIntracellular MembranesKidneyMembrane ProteinsOrthomyxoviridaeProtein Processing, Post-TranslationalRecombinant ProteinsTransfectionVesicular stomatitis Indiana virusViral Envelope ProteinsConceptsCarboxy-terminal portionGolgi apparatusMembrane proteinsChimeric geneTranscription-translation experimentsPlasma membrane proteinsEndoplasmic reticulum membraneMembrane protein segmentsVesicular stomatitis virus envelope glycoproteinER membraneHybrid proteinIntracellular sortingCytoplasmic portionJuxtanuclear regionTransmembrane portionMembrane polypeptidesCarboxy terminusChimeric proteinHybrid polypeptideInsertion signalProtein segmentsSecretory polypeptidesCytoplasmic vesiclesReticulum membraneER cisternae