2022
Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration
Rizzolo LJ, Nasonkin IO, Adelman RA. Retinal Cell Transplantation, Biomaterials, and In Vitro Models for Developing Next-generation Therapies of Age-related Macular Degeneration. Stem Cells Translational Medicine 2022, 11: 269-281. PMID: 35356975, PMCID: PMC8968686, DOI: 10.1093/stcltm/szac001.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationMacular degenerationPathology of AMDOuter blood-retinal barrierBlood-retinal barrierRetinal pigment epithelium cellsRetinal degenerative conditionsRisky surgical procedureCell replacement mechanismLoss of photoreceptorsRetinal cell transplantationPigment epithelium cellsCell therapy approachesRPE patchesNext-generation therapiesTransplant proceduresCell transplantationRPE transplantsClinical trialsSurgical proceduresApical processesBasal sideDegenerative conditionsRPE apical processesSevere impairment
2021
Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium
Cai H, Gong J, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium. Experimental Eye Research 2021, 207: 108576. PMID: 33895162, DOI: 10.1016/j.exer.2021.108576.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationRetinal pigment epitheliumMacular degenerationPigment epitheliumInduced pluripotent stem cellsEtiology of AMDMitochondrial dysfunctionAge-matched controlsNovel therapeutic targetTranscriptome of fibroblastsAMD patientsNormal donorsFibroblasts of patientsTherapeutic targetPatientsMore studiesAltered transcriptomeDisease phenotypeSignificant differencesCell linesMitochondrial functionDysfunctionOriginal fibroblastsDistinct transcriptomesDegeneration
2012
Effects of Proinflammatory Cytokines on the Claudin-19 Rich Tight Junctions of Human Retinal Pigment EpitheliumCytokine Effects on RPE Tight Junctions
Peng S, Gan G, Rao VS, Adelman RA, Rizzolo LJ. Effects of Proinflammatory Cytokines on the Claudin-19 Rich Tight Junctions of Human Retinal Pigment EpitheliumCytokine Effects on RPE Tight Junctions. Investigative Ophthalmology & Visual Science 2012, 53: 5016-5028. PMID: 22761260, PMCID: PMC3410691, DOI: 10.1167/iovs.11-8311.Peer-Reviewed Original ResearchConceptsHuman fetal RPEEffects of TNFαTransepithelial electrical resistanceProinflammatory cytokinesClaudin-19Tight junctionsAge-related macular degenerationZO-1Effects of cytokinesApical sideSubclinical inflammationTumor necrosisMacular degenerationTNFα receptorsOcular diseasesTNFαSerum-free mediumClaudin-3RPE tight junctionsFetal RPECytokinesClaudin-2Confocal immunofluorescence microscopyInhibitor of apoptosis