2016
P054 EDTA treatment of serum eliminates interference in flow cytometric crossmatches
Rewinski M, Ortiz J, Alberghini T, Bow L, Devivo J, Doyle P, Michalski T. P054 EDTA treatment of serum eliminates interference in flow cytometric crossmatches. Human Immunology 2016, 77: 78. DOI: 10.1016/j.humimm.2016.07.119.Peer-Reviewed Original ResearchT cell populationsPatient seraMedian fluorescence intensityT cellsComplement proteinsControl seraUntreated patients' seraFlow cytometric crossmatchDonor cellsSignificant T-cellCell populationsOccasional false positive resultsHigh-titer antibodiesPatient serum samplesCytometric crossmatchFlow crossmatchAntibody testingFalse-positive resultsIgM antibodiesPositive control seraMFI levelsNegative control seraTiter antibodiesSerum samplesSerum
2012
39-P INCREASING MFI CUTOFFS FOR UNACCEPTABLE ANTIGENS INCREASES ACCESS TO ACTUAL DECEASED DONOR CROSSMATCHES FOR THE SENSITIZED RECIPIENT
Miller M, Alberghini T, Rewinski M, Bow L. 39-P INCREASING MFI CUTOFFS FOR UNACCEPTABLE ANTIGENS INCREASES ACCESS TO ACTUAL DECEASED DONOR CROSSMATCHES FOR THE SENSITIZED RECIPIENT. Human Immunology 2012, 73: 74. DOI: 10.1016/j.humimm.2012.07.165.Peer-Reviewed Original ResearchUnacceptable antigensB cellsB-cell crossmatchPositive flow crossmatchLow-level antibodyHLA class IResultsFifty-sixSensitized recipientsKidney transplantationPositive crossmatchFlow crossmatchVirtual crossmatchDonor workupDP antibodiesNegative control seraLuminex assaysCrossmatchHigh MFI valuesControl seraFlow cytometryMultiple antibodiesClass IRecipientsAntibodiesMatch runs