2017
Current carried by the Slc26 family member prestin does not flow through the transporter pathway
Bai JP, Moeini-Naghani I, Zhong S, Li FY, Bian S, Sigworth FJ, Santos-Sacchi J, Navaratnam D. Current carried by the Slc26 family member prestin does not flow through the transporter pathway. Scientific Reports 2017, 7: 46619. PMID: 28422190, PMCID: PMC5395958, DOI: 10.1038/srep46619.Peer-Reviewed Original Research
2011
Chloride Dependent Coupling of Molecular to Cellular Mechanics in the Outer Hair Cell of Corti's Organ
Song L, Santos-Sacchi J, Shera C, Olson E. Chloride Dependent Coupling of Molecular to Cellular Mechanics in the Outer Hair Cell of Corti's Organ. AIP Conference Proceedings 2011, 1403: 179-184. DOI: 10.1063/1.3658083.Peer-Reviewed Original ResearchCellular mechanicsConformational changesMotor protein prestinProtein prestinMolecular eventsLateral membranesOHC lateral membraneCellular expressionWhole cellsElectromotilityOuter Hair CellsPrestinHair cellsReceptor potentialNonlinear capacitanceMolecular conformational changesIntracellular chloride levelsCellsTight couplingOrgansOHC receptor potentialsExpressionMembraneNormal levelsVivo
2007
Developmental Expression of the Outer Hair Cell Motor Prestin in the Mouse
Abe T, Kakehata S, Kitani R, Maruya S, Navaratnam D, Santos-Sacchi J, Shinkawa H. Developmental Expression of the Outer Hair Cell Motor Prestin in the Mouse. The Journal Of Membrane Biology 2007, 215: 49-56. PMID: 17415610, PMCID: PMC4154540, DOI: 10.1007/s00232-007-9004-5.Peer-Reviewed Original ResearchConceptsLateral membranesOuter hair cellsMouse outer hair cellsMotor protein activitySteady-state levelsKnockout experimentsProtein activityDevelopmental expressionMembrane tensionGenetic modificationConformational fluctuationsCellular mechanismsMotor moleculesMolecular motorsOnset of hearingMorphological estimatesProtein densityEarly maturationQuantitative polymerase chain reactionHair cellsVpkCmDevelopmental dataCochlear amplificationMaturationFull maturation
2004
On Membrane Motor Activity and Chloride Flux in the Outer Hair Cell: Lessons Learned from the Environmental Toxin Tributyltin
Song L, Seeger A, Santos-Sacchi J. On Membrane Motor Activity and Chloride Flux in the Outer Hair Cell: Lessons Learned from the Environmental Toxin Tributyltin. Biophysical Journal 2004, 88: 2350-2362. PMID: 15596517, PMCID: PMC1305283, DOI: 10.1529/biophysj.104.053579.Peer-Reviewed Original ResearchConceptsLateral membranesCell’s mechanical activityOuter hair cellsMammalian cochlear amplifierHair cellsMembrane motorMarine mammalsIntact outer hair cellsPotent abilityFood chainNonselective conductanceCl(-) homeostasisPrestinNanomolar concentrationsExtracellular Cl- concentrationCl fluxMembraneCellsNew environmental threatsMammalsChloride effectTributyltinEnvironmental threatsHomeostasisNonlinear capacitance
2003
Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility
Santos-Sacchi J, Zhao HB. Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility. Pflügers Archiv - European Journal Of Physiology 2003, 446: 617-622. PMID: 12783229, DOI: 10.1007/s00424-003-1053-8.Peer-Reviewed Original ResearchCl− flux through a non‐selective, stretch‐sensitive conductance influences the outer hair cell motor of the guinea‐pig
Rybalchenko V, Santos‐Sacchi J. Cl− flux through a non‐selective, stretch‐sensitive conductance influences the outer hair cell motor of the guinea‐pig. The Journal Of Physiology 2003, 547: 873-891. PMID: 12562920, PMCID: PMC2342734, DOI: 10.1111/j.1469-7793.2003.00873.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarbenoxoloneCell MembraneChloride ChannelsChloridesCyclooxygenase InhibitorsElectric CapacitanceElectric ConductivityGadoliniumGuinea PigsHair Cells, Auditory, OuterIon Channel GatingMechanotransduction, CellularMembrane PotentialsMercuric ChlorideMicrodialysisNiflumic AcidOctanolsProteinsSulfonic AcidsConceptsOuter hair cell motorLateral membranesVoltage-dependent conformational changesLateral plasma membranePlasma membraneMotor proteinsCell motorProtein transitionsConformational changesRecent identificationPrestinSomatic motilityAllosteric modulationCL sensitivityAnion fluxHair cellsCochlear amplificationAcoustic ratesOuter hair cellsAlternative mechanismComplicated roleMembranePronounced shiftCellsAmplification
2002
Functional motor microdomains of the outer hair cell lateral membrane
Santos-Sacchi J. Functional motor microdomains of the outer hair cell lateral membrane. Pflügers Archiv - European Journal Of Physiology 2002, 445: 331-336. PMID: 12466934, DOI: 10.1007/s00424-002-0928-4.Peer-Reviewed Original ResearchConceptsOperating voltage rangeLateral membranesMammalian inner earOuter hair cell lateral membraneOuter hair cellsMotor proteinsFunctional variationMechanical deformationNeuroepithelial cellsVoltage rangeMicrodomainsWhole cellsMidpoint voltageHair cellsMotor chargeCell characteristicsMembraneCellsNonlinear capacitanceInner ear
2001
Non-uniform mapping of stress-induced, motility-related charge movement in the outer hair cell plasma membrane
Takahashi S, Santos-Sacchi J. Non-uniform mapping of stress-induced, motility-related charge movement in the outer hair cell plasma membrane. Pflügers Archiv - European Journal Of Physiology 2001, 441: 506-513. PMID: 11212214, DOI: 10.1007/s004240000455.Peer-Reviewed Original ResearchConceptsCell plasma membranePlasma membraneLateral membranesOuter hair cell plasma membraneVoltage-dependent motilityMammalian cochlear amplifierOuter hair cell electromotilityMembrane microdomainsGating currentsHair cell electromotilityTurgor pressureOHC motorMolecular motorsElectromotile responseMembraneMotilityCytoskeletonCharge movementBell-shaped distributionMicrodomainsElectromotilityNon-uniform mappingPronase treatmentCochlear amplifierPronase
1997
Mapping the distribution of outer hair cell voltage-dependent conductances by electrical amputation
Santos-Sacchi J, Huang GJ, Wu M. Mapping the distribution of outer hair cell voltage-dependent conductances by electrical amputation. Biophysical Journal 1997, 73: 1424-1429. PMID: 9284309, PMCID: PMC1181041, DOI: 10.1016/s0006-3495(97)78174-8.Peer-Reviewed Original ResearchConceptsLateral membranesVoltage magnitudeElectrical techniquesAcoustic frequencyKilohertz rangeOuter hair cell functionVoltage-dependent conductancesVoltage sensorPlasma membraneHair cell functionMechanical effectorsSynaptic poleVoltage-dependent currentsMembrane moleculesAuditory functionDense populationCell functionCable propertiesCochlear amplifierOHC motilitySensory receptors