2021
Aneuploidy as a promoter and suppressor of malignant growth
Vasudevan A, Schukken KM, Sausville EL, Girish V, Adebambo OA, Sheltzer JM. Aneuploidy as a promoter and suppressor of malignant growth. Nature Reviews Cancer 2021, 21: 89-103. PMID: 33432169, DOI: 10.1038/s41568-020-00321-1.Peer-Reviewed Original Research
2020
Discovering and validating cancer genetic dependencies: approaches and pitfalls
Lin A, Sheltzer JM. Discovering and validating cancer genetic dependencies: approaches and pitfalls. Nature Reviews Genetics 2020, 21: 671-682. PMID: 32561862, DOI: 10.1038/s41576-020-0247-7.Peer-Reviewed Original ResearchSingle-Chromosomal Gains Can Function as Metastasis Suppressors and Promoters in Colon Cancer
Vasudevan A, Baruah PS, Smith JC, Wang Z, Sayles NM, Andrews P, Kendall J, Leu J, Chunduri NK, Levy D, Wigler M, Storchová Z, Sheltzer JM. Single-Chromosomal Gains Can Function as Metastasis Suppressors and Promoters in Colon Cancer. Developmental Cell 2020, 52: 413-428.e6. PMID: 32097652, PMCID: PMC7354079, DOI: 10.1016/j.devcel.2020.01.034.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyAnimalsApoptosisCell MovementCell ProliferationChromosomal InstabilityChromosomes, Human, Pair 5Colonic NeoplasmsEpithelial-Mesenchymal TransitionFemaleHumansMaleMembrane ProteinsMiceMice, NudeNeoplasm InvasivenessNucleotidyltransferasesTumor Cells, CulturedXenograft Model Antitumor AssaysConceptsSingle extra chromosomeCell state transitionsCGAS/STING signalingCell linesCopy number dataPartial epithelial-mesenchymal transitionCancer aneuploidyPhenotypic plasticityGenomic plasticitySingle chromosomeEpithelial-mesenchymal transitionMetastasis suppressorChromosomal instabilityExtra chromosomeCertain aneuploidiesDifferent aneuploidiesCancer progressionSpecific aneuploidiesChromosomal gainsChromosomesSTING signalingMetastatic behaviorTumor progressionAneuploidyUniform driver
2019
Micronuclei-based model system reveals functional consequences of chromothripsis in human cells
Kneissig M, Keuper K, de Pagter MS, van Roosmalen MJ, Martin J, Otto H, Passerini V, Sparr A, Renkens I, Kropveld F, Vasudevan A, Sheltzer JM, Kloosterman WP, Storchova Z. Micronuclei-based model system reveals functional consequences of chromothripsis in human cells. ELife 2019, 8: e50292. PMID: 31778112, PMCID: PMC6910827, DOI: 10.7554/elife.50292.Peer-Reviewed Original ResearchConceptsMassive chromosomal rearrangementsChromosomal rearrangementsHuman cellsLamin B1Replication-dependent mechanismModel systemMicronucleus sizeProper assemblyAberrant replicationChromosome shatteringChromosome transferMembrane curvatureNuclear envelopeExtra chromosomeAberrant structuresDNA damageChromosomesGrowth advantageFunctional consequencesCancer cellsAbnormal numberTrisomic cellsCellsChromosomal aberrationsRearrangementGenerating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
Giuliano CJ, Lin A, Girish V, Sheltzer JM. Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9. Current Protocols In Molecular Biology 2019, 128: e100. PMID: 31503414, PMCID: PMC6741428, DOI: 10.1002/cpmb.100.Peer-Reviewed Original ResearchConceptsKnockout clonesMammalian cellsCell linesCRISPR/Cas9 technologyGuide RNA designMammalian cell linesGene lossClonal cell linesGene functionProtein functionGene targetingNew cell lineCas9 technologyTargeted geneFunction mutationsInterclonal heterogeneityRNA designSingle cellsSuccessful derivationClonesCRISPRCRISPR deliveryBiological reagentsRapid generationMutations
2018
MELK expression correlates with tumor mitotic activity but is not required for cancer growth
Giuliano C, Lin A, Smith J, Palladino A, Sheltzer J. MELK expression correlates with tumor mitotic activity but is not required for cancer growth. ELife 2018, 7: e32838. PMID: 29417930, PMCID: PMC5805410, DOI: 10.7554/elife.32838.Peer-Reviewed Original ResearchConceptsMaternal embryonic leucine zipper kinaseTumor mitotic activityCancer typesMitotic activityPoor clinical prognosisBreast cancer cell linesPromising therapeutic targetTriple-negative breast cancer cell linesEmbryonic leucine zipper kinaseMultiple cancer typesLeucine zipper kinaseCancer cell linesCytotoxic chemotherapyAggressive diseaseCancer patientsClinical prognosisMELK expressionTherapeutic targetChemotherapy resistanceCancer growthTumor growthAcute inhibitionMELK inhibitorExpression correlatesCancer-related processes
2017
Single-chromosome Gains Commonly Function as Tumor Suppressors
Sheltzer J, Ko J, Replogle J, Burgos N, Chung E, Meehl C, Sayles N, Passerini V, Storchova Z, Amon A. Single-chromosome Gains Commonly Function as Tumor Suppressors. Cancer Cell 2017, 31: 240-255. PMID: 28089890, PMCID: PMC5713901, DOI: 10.1016/j.ccell.2016.12.004.Peer-Reviewed Original ResearchConceptsSingle chromosome gainsSingle extra chromosomeEffects of aneuploidyHallmarks of cancerEvolutionary flexibilityFitness defectsEuploid cellsTumor suppressorExtra chromosomeEuploid counterpartsOncogenic pathwaysProlonged growthChromosomal alterationsCancer developmentCell linesTrisomic cellsImproved fitnessAneuploidyTrisomic cell lineCellsChromosomesSuppressorAdditional chromosomal alterationsGrowthTumorigenesis
2012
Transcriptional consequences of aneuploidy
Sheltzer J, Torres E, Dunham M, Amon A. Transcriptional consequences of aneuploidy. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 12644-12649. PMID: 22802626, PMCID: PMC3411958, DOI: 10.1073/pnas.1209227109.Peer-Reviewed Original ResearchConceptsConsequences of aneuploidyRelated gene expression patternsAneuploid cellsSimilar cellular pathwaysGene expression patternsGene expression dataDiverse organismsDifferent organismsTranscriptional consequencesDifferent chromosomesCell physiologyCellular pathwaysExpression patternsGene expressionCell cycleExpression dataDifferent aneuploidiesAntiproliferative responseCell proliferationGenesOrganismsSpeciesAneuploidySpecific chromosomal aberrationsChromosomal aberrations