Yannick Jacob, PhD
Associate Professor Tenure of Molecular, Cellular and Developmental BiologyCards
About
Research
Publications
2023
H3.1K27me1 loss confers Arabidopsis resistance to Geminivirus by sequestering DNA repair proteins onto host genome
Wang Z, Castillo-González C, Zhao C, Tong C, Li C, Zhong S, Liu Z, Xie K, Zhu J, Wu Z, Peng X, Jacob Y, Michaels S, Jacobsen S, Zhang X. H3.1K27me1 loss confers Arabidopsis resistance to Geminivirus by sequestering DNA repair proteins onto host genome. Nature Communications 2023, 14: 7484. PMID: 37980416, PMCID: PMC10657422, DOI: 10.1038/s41467-023-43311-1.Peer-Reviewed Original ResearchConceptsDNA repair proteinsDNA repair pathwaysRepair pathwaysRepair proteinsWild-type plantsDefense-related genesArabidopsis resistanceHeterochromatin amplificationHeterochromatic regionsRAD51 recruitmentRep proteinGenomic instabilityHealthy plantsUnstable genomeHost genomeATXR6GeminivirusesRobust resistanceProteinViral amplificationGenomeMutantsViral DNATransposonPlants
2022
The histone H3.1 variant regulates TONSOKU-mediated DNA repair during replication
Davarinejad H, Huang YC, Mermaz B, LeBlanc C, Poulet A, Thomson G, Joly V, Muñoz M, Arvanitis-Vigneault A, Valsakumar D, Villarino G, Ross A, Rotstein BH, Alarcon EI, Brunzelle JS, Voigt P, Dong J, Couture JF, Jacob Y. The histone H3.1 variant regulates TONSOKU-mediated DNA repair during replication. Science 2022, 375: 1281-1286. PMID: 35298257, PMCID: PMC9153895, DOI: 10.1126/science.abm5320.Peer-Reviewed Original ResearchConceptsTetratricopeptide repeat domainDNA polymerase thetaMulticellular eukaryotesHistone H3.1Replication forksChromatin maturationRepeat domainDNA repairGenomic instabilityPolymerase thetaPosition 31Amino acidsH3.1PlantsReplicationEukaryotesH3.3HistonesMonomethylationVariantsCommon strategyForkResiduesMaturationFunction
2021
H3.1K27me1 maintains transcriptional silencing and genome stability by preventing GCN5-mediated histone acetylation
Dong J, LeBlanc C, Poulet A, Mermaz B, Villarino G, Webb KM, Joly V, Mendez J, Voigt P, Jacob Y. H3.1K27me1 maintains transcriptional silencing and genome stability by preventing GCN5-mediated histone acetylation. The Plant Cell 2021, 33: 961-979. PMID: 33793815, PMCID: PMC8226292, DOI: 10.1093/plcell/koaa027.Peer-Reviewed Original ResearchConceptsGenome stabilityGenomic instabilityHistone acetylationSAGA-like complexesMultiple lysine residuesArabidopsis GCN5ARABIDOPSIS TRITHORAXArabidopsis thalianaTranscriptional silencingHeterochromatin defectsDouble mutantDNA replicationEpigenetic mechanismsGCN5Molecular roleEssential functionsDiverse rolesMolecular mechanismsLysine residuesProtein 5AcetylationMutantsPlantsADA2bATXR6
2017
Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress
LeBlanc C, Zhang F, Mendez J, Lozano Y, Chatpar K, Irish V, Jacob Y. Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress. The Plant Journal 2017, 93: 377-386. PMID: 29161464, DOI: 10.1111/tpj.13782.Peer-Reviewed Original ResearchConceptsCRISPR/Green fluorescent protein (GFP) reporter geneCRISPR/Cas9 systemFluorescent protein reporter geneCRISPR/Cas9Off-target mutationsArabidopsis plantsEukaryotic genomesDifferent organismsSomatic tissuesCitrus plantsCas9 systemDNA breaksReporter geneTarget mutagenesisTargeted mutationsMutation rateMutagenesisImportance of temperatureArabidopsisHeat stressPlantsMutationsQuantitative assayEukaryotes
2014
Selective Methylation of Histone H3 Variant H3.1 Regulates Heterochromatin Replication
Jacob Y, Bergamin E, Donoghue MT, Mongeon V, LeBlanc C, Voigt P, Underwood CJ, Brunzelle JS, Michaels SD, Reinberg D, Couture JF, Martienssen RA. Selective Methylation of Histone H3 Variant H3.1 Regulates Heterochromatin Replication. Science 2014, 343: 1249-1253. PMID: 24626927, PMCID: PMC4049228, DOI: 10.1126/science.1248357.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceArabidopsisArabidopsis ProteinsCatalytic DomainConserved SequenceCrystallography, X-RayDNA ReplicationEpigenesis, GeneticGene Expression Regulation, PlantHeterochromatinHistonesMethylationMethyltransferasesMitosisMolecular Sequence DataProtein Processing, Post-TranslationalThreonineConceptsHistone H3 variantWidespread regulatory functionsHistone-modifying enzymesARABIDOPSIS TRITHORAXMitotic inheritanceH3 variantHistone variantsHeterochromatin replicationSET domainDNA replicationPosttranslational modificationsCatalytic domainRegulatory functionsPosition 31Protein 5ATXR5H3.3Selective methylationReplicationATXR6TrithoraxH3K27me1ParalogsHeterochromatizationH3.1
2010
Regulation of heterochromatic DNA replication by histone H3 lysine 27 methyltransferases
Jacob Y, Stroud H, LeBlanc C, Feng S, Zhuo L, Caro E, Hassel C, Gutierrez C, Michaels SD, Jacobsen SE. Regulation of heterochromatic DNA replication by histone H3 lysine 27 methyltransferases. Nature 2010, 466: 987-991. PMID: 20631708, PMCID: PMC2964344, DOI: 10.1038/nature09290.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsArabidopsisArabidopsis ProteinsCatalytic DomainDNA MethylationDNA ReplicationDNA Transposable ElementsDNA, PlantGene Expression Regulation, PlantGene SilencingGenome, PlantHeterochromatinHistone-Lysine N-MethyltransferaseHistonesLysineMethylationMethyltransferasesMutant ProteinsMutationReplication Origin
2009
ATXR5 and ATXR6 are H3K27 monomethyltransferases required for chromatin structure and gene silencing
Jacob Y, Feng S, LeBlanc CA, Bernatavichute YV, Stroud H, Cokus S, Johnson LM, Pellegrini M, Jacobsen SE, Michaels SD. ATXR5 and ATXR6 are H3K27 monomethyltransferases required for chromatin structure and gene silencing. Nature Structural & Molecular Biology 2009, 16: 763-768. PMID: 19503079, PMCID: PMC2754316, DOI: 10.1038/nsmb.1611.Peer-Reviewed Original Research