Illumina HiSeq2500, HiSeq4000, and NovaSeq

The HiSeq 2500 System is a powerful and efficient ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. Unrivaled data quality using Illumina's proven SBS chemistry has made the HiSeq 2500 the instrument of choice for all major genome centers and leading institutions throughout the world. New HiSeq v4 reagents allow for more reads and more data in less time.

Building on the proven HiSeq 2500 System and leveraging innovative patterned flow cell technology, the HiSeq 4000 system provides improved speed and performance. 

The NovaSeq 6000 further improves on Illumina's patterned flow cell technology.  The dual-flow system delivers the highest throughput and lowest price per sample across multiple applications.


YCGA Sequencer Specifications


Sequencer Read length # of Clusters per lane (millions) Bases per lane (Gbp)
HiSeq 2500 Rapid 1x75 150 11.25
HiSeq 2500 Rapid 2x75 150 22.5
HiSeq 2500 Rapid 2x150 150 45
HiSeq 2500 High-output  1x75 200 15
HiSeq 2500 High-output  2x75 200 30
HiSeq 4000 2x100 300 60
HiSeq 4000 2x150 300 90
NovaSeq S2 2x100 1650 330
NovaSeq S2 2x150 1650 500
NovaSeq S4 2x150 2000 600