2004
A DNA polymerase β mutant from colon cancer cells induces mutations
Lang T, Maitra M, Starcevic D, Li SX, Sweasy JB. A DNA polymerase β mutant from colon cancer cells induces mutations. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 6074-6079. PMID: 15075389, PMCID: PMC395925, DOI: 10.1073/pnas.0308571101.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceColonic NeoplasmsDNA Polymerase betaDNA PrimersMolecular Sequence DataMutagenesisReverse Transcriptase Polymerase Chain ReactionTranscription, GeneticConceptsMisincorporation of nucleotidesK289MM proteinPol beta proteinC base substitutionMouse L cellsMutation frequencyColon cancer cellsSpontaneous mutation frequencyDNA pol betaDNA positioningLys-289Mutational targetMouse cellsAltered positioningBase substitutionsColorectal carcinomaBacterial cellsTarget sequencePol betaProteinTemplate GLambda DNANeoplastic progressionBeta protein
2001
A DNA Polymerase β Mutator Mutant with Reduced Nucleotide Discrimination and Increased Protein Stability † , ‡
Shah A, Conn D, Li S, Capaldi A, Jäger J, Sweasy J. A DNA Polymerase β Mutator Mutant with Reduced Nucleotide Discrimination and Increased Protein Stability † , ‡. Biochemistry 2001, 40: 11372-11381. PMID: 11560485, DOI: 10.1021/bi010755y.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionBase SequenceDeoxyribonucleotidesDNA Mutational AnalysisDNA Polymerase betaDNA, BacterialEnzyme StabilityEscherichia coliFrameshift MutationGene LibraryGenotypeHot TemperatureKineticsModels, ChemicalModels, MolecularMolecular Sequence DataMutationNucleic Acid ConformationPoint MutationProtein ConformationProtein DenaturationSimplexvirusSubstrate SpecificityThermodynamicsThymidine KinaseUreaConceptsNucleotide discriminationPol betaIncreased Protein StabilityVivo genetic screenHydrophobic amino acid residuesDeoxynucleoside triphosphate substratesProtein conformational changesAmino acid residuesC-terminal portionWild-type pol betaDNA synthesis fidelityPhosphodiester bond formationGenetic screenDNA polymerase betaSubstrate discriminationMutator mutantsGround-state bindingHigh mutation frequencyPolymerase structureProtein stabilityMutant enzymesStructural basisTransient-state kinetic methodsAcid residuesMutator activity