2002
Threonine 79 Is a Hinge Residue That Governs the Fidelity of DNA Polymerase β by Helping to Position the DNA within the Active Site*
Maitra M, Gudzelak A, Li SX, Matsumoto Y, Eckert KA, Jager J, Sweasy JB. Threonine 79 Is a Hinge Residue That Governs the Fidelity of DNA Polymerase β by Helping to Position the DNA within the Active Site*. Journal Of Biological Chemistry 2002, 277: 35550-35560. PMID: 12121998, DOI: 10.1074/jbc.m204953200.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesDNA Polymerase betaDNA PrimersDNA ReplicationDNA, BacterialFrameshift MutationPhenotypeThreonineConceptsHelix motifPol betaDNA synthesisThr-79Vivo genetic screenAccurate DNA synthesisDifferent amino acid residuesAmino acid residuesWild-type pol betaN-terminal 8DNA polymerase βGenetic screenDNA polymerase betaAntimutator phenotypeDNA substratesIncoming dNTP substrateActive siteWild typeBent DNAHinge residuesAcid residuesDNA templateBeta enzymePolymerase βPolymerase beta
2001
A DNA Polymerase β Mutator Mutant with Reduced Nucleotide Discrimination and Increased Protein Stability † , ‡
Shah A, Conn D, Li S, Capaldi A, Jäger J, Sweasy J. A DNA Polymerase β Mutator Mutant with Reduced Nucleotide Discrimination and Increased Protein Stability † , ‡. Biochemistry 2001, 40: 11372-11381. PMID: 11560485, DOI: 10.1021/bi010755y.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionBase SequenceDeoxyribonucleotidesDNA Mutational AnalysisDNA Polymerase betaDNA, BacterialEnzyme StabilityEscherichia coliFrameshift MutationGene LibraryGenotypeHot TemperatureKineticsModels, ChemicalModels, MolecularMolecular Sequence DataMutationNucleic Acid ConformationPoint MutationProtein ConformationProtein DenaturationSimplexvirusSubstrate SpecificityThermodynamicsThymidine KinaseUreaConceptsNucleotide discriminationPol betaIncreased Protein StabilityVivo genetic screenHydrophobic amino acid residuesDeoxynucleoside triphosphate substratesProtein conformational changesAmino acid residuesC-terminal portionWild-type pol betaDNA synthesis fidelityPhosphodiester bond formationGenetic screenDNA polymerase betaSubstrate discriminationMutator mutantsGround-state bindingHigh mutation frequencyPolymerase structureProtein stabilityMutant enzymesStructural basisTransient-state kinetic methodsAcid residuesMutator activity