2004
A DNA polymerase β mutant from colon cancer cells induces mutations
Lang T, Maitra M, Starcevic D, Li SX, Sweasy JB. A DNA polymerase β mutant from colon cancer cells induces mutations. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 6074-6079. PMID: 15075389, PMCID: PMC395925, DOI: 10.1073/pnas.0308571101.Peer-Reviewed Original ResearchConceptsMisincorporation of nucleotidesK289MM proteinPol beta proteinC base substitutionMouse L cellsMutation frequencyColon cancer cellsSpontaneous mutation frequencyDNA pol betaDNA positioningLys-289Mutational targetMouse cellsAltered positioningBase substitutionsColorectal carcinomaBacterial cellsTarget sequencePol betaProteinTemplate GLambda DNANeoplastic progressionBeta protein
2002
Threonine 79 Is a Hinge Residue That Governs the Fidelity of DNA Polymerase β by Helping to Position the DNA within the Active Site*
Maitra M, Gudzelak A, Li SX, Matsumoto Y, Eckert KA, Jager J, Sweasy JB. Threonine 79 Is a Hinge Residue That Governs the Fidelity of DNA Polymerase β by Helping to Position the DNA within the Active Site*. Journal Of Biological Chemistry 2002, 277: 35550-35560. PMID: 12121998, DOI: 10.1074/jbc.m204953200.Peer-Reviewed Original ResearchConceptsHelix motifPol betaDNA synthesisThr-79Vivo genetic screenAccurate DNA synthesisDifferent amino acid residuesAmino acid residuesWild-type pol betaN-terminal 8DNA polymerase βGenetic screenDNA polymerase betaAntimutator phenotypeDNA substratesIncoming dNTP substrateActive siteWild typeBent DNAHinge residuesAcid residuesDNA templateBeta enzymePolymerase βPolymerase beta