2001
A DNA Polymerase β Mutator Mutant with Reduced Nucleotide Discrimination and Increased Protein Stability † , ‡
Shah A, Conn D, Li S, Capaldi A, Jäger J, Sweasy J. A DNA Polymerase β Mutator Mutant with Reduced Nucleotide Discrimination and Increased Protein Stability † , ‡. Biochemistry 2001, 40: 11372-11381. PMID: 11560485, DOI: 10.1021/bi010755y.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionBase SequenceDeoxyribonucleotidesDNA Mutational AnalysisDNA Polymerase betaDNA, BacterialEnzyme StabilityEscherichia coliFrameshift MutationGene LibraryGenotypeHot TemperatureKineticsModels, ChemicalModels, MolecularMolecular Sequence DataMutationNucleic Acid ConformationPoint MutationProtein ConformationProtein DenaturationSimplexvirusSubstrate SpecificityThermodynamicsThymidine KinaseUreaConceptsNucleotide discriminationPol betaIncreased Protein StabilityVivo genetic screenHydrophobic amino acid residuesDeoxynucleoside triphosphate substratesProtein conformational changesAmino acid residuesC-terminal portionWild-type pol betaDNA synthesis fidelityPhosphodiester bond formationGenetic screenDNA polymerase betaSubstrate discriminationMutator mutantsGround-state bindingHigh mutation frequencyPolymerase structureProtein stabilityMutant enzymesStructural basisTransient-state kinetic methodsAcid residuesMutator activity
1997
A genetic system to identify DNA polymerase β mutator mutants
Washington S, Yoon M, Chagovetz A, Li S, Clairmont C, Preston B, Eckert K, Sweasy J. A genetic system to identify DNA polymerase β mutator mutants. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 1321-1326. PMID: 9037051, PMCID: PMC19789, DOI: 10.1073/pnas.94.4.1321.Peer-Reviewed Original ResearchConceptsMutator mutantsGenetic methodsPol betaDNA synthesisAccurate DNA synthesisDNA repair processesRat pol betaWild-type pol betaEscherichia coli DNA polymerase ISpontaneous mutation frequencyDNA polymerase IAltered fidelityDNA polymerase betaMutant proteinsDNA replicationGenetic systemMammalian cellsGenetic assaysPolymerase IMutantsDNA polymerase mutantsMutator activityPolymerase mutantsBeta enzymePolymerase beta