2012
Pathogenesis in tuberculosis: transcriptomic approaches to unraveling virulence mechanisms and finding new drug targets
Mukhopadhyay S, Nair S, Ghosh S. Pathogenesis in tuberculosis: transcriptomic approaches to unraveling virulence mechanisms and finding new drug targets. FEMS Microbiology Reviews 2012, 36: 463-485. PMID: 22092372, DOI: 10.1111/j.1574-6976.2011.00302.x.Peer-Reviewed Original ResearchConceptsMajor health problemHost immune responseEmergence of strainsDrug targetsDendritic cellsEffective drug targetsInhibition of apoptosisImmune responseNew drug targetsHealth problemsLipid metabolismMultiple drugsTuberculosisComplex etiologyMycobacterium tuberculosisIntracellular life styleTranscriptome signaturesVirulence mechanismsGenome-wide expression profilingDrugsAntibiotic drugsExpression profilingProtein secretionLife stylePoor understanding
2011
The PPE18 Protein of Mycobacterium tuberculosis Inhibits NF-κB/rel–Mediated Proinflammatory Cytokine Production by Upregulating and Phosphorylating Suppressor of Cytokine Signaling 3 Protein
Nair S, Pandey AD, Mukhopadhyay S. The PPE18 Protein of Mycobacterium tuberculosis Inhibits NF-κB/rel–Mediated Proinflammatory Cytokine Production by Upregulating and Phosphorylating Suppressor of Cytokine Signaling 3 Protein. The Journal Of Immunology 2011, 186: 5413-5424. PMID: 21451109, DOI: 10.4049/jimmunol.1000773.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, BacterialBacterial ProteinsBlotting, WesternCell LineCell SeparationCytokinesElectrophoretic Mobility Shift AssayFlow CytometryHumansImmunoprecipitationInflammationMacrophagesMycobacterium tuberculosisNF-kappa BPhosphorylationReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingSignal TransductionSuppressor of Cytokine Signaling 3 ProteinSuppressor of Cytokine Signaling ProteinsTranscription Factor RelATransfectionTuberculosisUp-RegulationConceptsTranscription factorsNF-κB/Rel transcription factorsNuclear translocationNF-κB/RelRel transcription factorsSubsequent intracellular survivalC-Rel transcription factorPhosphorylation sitesTyrosine phosphorylationSpecific knockdownSuccessful infectionIntracellular survivalRel subunitsCytokine signaling-3 (SOCS-3) proteinNovel mechanismIκB kinasePhosphorylationProteinNuclear levelsNF-κB activationSOCS3Phosphorylation of IκBαSuppressorTNF-α productionTranslocation
2009
The PPE18 of Mycobacterium tuberculosis Interacts with TLR2 and Activates IL-10 Induction in Macrophage
Nair S, Ramaswamy PA, Ghosh S, Joshi DC, Pathak N, Siddiqui I, Sharma P, Hasnain SE, Mande SC, Mukhopadhyay S. The PPE18 of Mycobacterium tuberculosis Interacts with TLR2 and Activates IL-10 Induction in Macrophage. The Journal Of Immunology 2009, 183: 6269-6281. PMID: 19880448, DOI: 10.4049/jimmunol.0901367.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, BacterialBacterial ProteinsCell LineCytokinesExtracellular Signal-Regulated MAP KinasesHumansInterleukin-10LipopolysaccharidesMacrophage ActivationMacrophagesMAP Kinase Kinase 4MonocytesMycobacterium tuberculosisP38 Mitogen-Activated Protein KinasesRecombinant ProteinsSignal TransductionToll-Like Receptor 2TuberculosisConceptsIL-10 inductionIL-10Proline-glutamic acidTh2-type responseIL-10 productionAnti-inflammatory responseMycobacterium tuberculosis strainsTHP-1 macrophagesPPE18 proteinInfected macrophagesTuberculosis strainsTLR2PPE18Synthetic lipopeptideMycobacterium tuberculosisMacrophagesMycobacterium tuberculosis interactsERK 1/2M. smegmatis strainPathophysiological functionsP38 MAPKType responseSilico docking analysisSustained activationPoor level
2008
Association of Strong Immune Responses to PPE Protein Rv1168c with Active Tuberculosis ▿
Khan N, Alam K, Nair S, Valluri VL, Murthy KJ, Mukhopadhyay S. Association of Strong Immune Responses to PPE Protein Rv1168c with Active Tuberculosis ▿. MSphere 2008, 15: 974-980. PMID: 18400969, PMCID: PMC2446626, DOI: 10.1128/cvi.00485-07.Peer-Reviewed Original ResearchConceptsTB patientsESAT-6Sensitized peripheral blood mononuclear cellsStrong gamma interferon responseSmear-negative pulmonary TBPeripheral blood mononuclear cellsExtrapulmonary TB casesSerodiagnosis of TBBlood mononuclear cellsPotent T-cell antigenControl of TBT-cell antigensGamma interferon responseStrong immune responseConventional diagnostic testsConventional enzyme immunoassayActive TBMycobacterium bovis BCGPulmonary TBActive tuberculosisTB casesTuberculosis infectionMononuclear cellsImmune responseStrong immunoreactivity