2017
Deoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells
Kim S, Son K, Kim K, Yu S, Park S, Kim Y, Nam H, Suh J, Ji J, Ahn S. Deoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells. Pharmacological Reports 2017, 69: 878-884. PMID: 28623712, DOI: 10.1016/j.pharep.2017.04.007.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic Agents, PhytogenicApoptosisAutophagyBone NeoplasmsCell Line, TumorCell SurvivalDrugs, Chinese HerbalGene Expression RegulationHumansOsteosarcomaPhosphoinositide-3 Kinase InhibitorsPodophyllotoxinProto-Oncogene Proteins c-aktReactive Oxygen SpeciesTOR Serine-Threonine KinasesConceptsReactive oxygen speciesPI3K/AKT/mTOR pathwayAKT/mTOR pathwayU2OS cellsAcidic vesicular organelles (AVOs) formationMTOR pathwayMitochondrial reactive oxygen speciesOsteosarcoma U2OS cellsCell death modeOxygen speciesPropidium iodidePI3K/Akt/mTORCell cycle arrestLight chain 3Cell viabilityCellular processesInhibition of apoptosisAkt/mTOROrganelle formationAcridine orange stainingAutophagy processApoptotic responseDeath modeWestern blot analysisCell survival
2016
Toyocamycin induces apoptosis via the crosstalk between reactive oxygen species and p38/ERK MAPKs signaling pathway in human prostate cancer PC-3 cells
Park S, Kim S, Kim K, Yu S, Choi H, Kim Y, Nam H, Seo Y, Ahn S. Toyocamycin induces apoptosis via the crosstalk between reactive oxygen species and p38/ERK MAPKs signaling pathway in human prostate cancer PC-3 cells. Pharmacological Reports 2016, 69: 90-96. PMID: 27912102, DOI: 10.1016/j.pharep.2016.10.014.Peer-Reviewed Original ResearchConceptsProstate cancer PC-3 cellsPC-3 cellsCancer PC-3 cellsOxygen species productionROS productionERK MAPKNon-malignant RWPE-1 cellsExtracellular signal-regulated kinase (ERK) activityMitochondrial dysfunctionSpecies productionMitochondrial membrane potentialCell cycle arrestHuman prostate cancer PC-3 cellsReactive oxygen species productionCell viabilityRWPE-1 cellsProtein kinaseStreptomyces speciesKinase activityReactive oxygen speciesERK activationMolecular mechanismsP38 activationMAPK proteinsApoptotic effectsSilibinin induces mitochondrial NOX4-mediated endoplasmic reticulum stress response and its subsequent apoptosis
Kim S, Kim K, Yu S, Seo Y, Chun S, Yu H, Ahn S. Silibinin induces mitochondrial NOX4-mediated endoplasmic reticulum stress response and its subsequent apoptosis. BMC Cancer 2016, 16: 452. PMID: 27405931, PMCID: PMC4942927, DOI: 10.1186/s12885-016-2516-6.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesNOX4 expressionDisruption of Ca2ER stress responseProstate cancer PC-3 cellsCancer PC-3 cellsRegulation of NOX4Inhibited tumor growthCell linesPC-3 cellsProstate cell linesSilibinin-induced apoptosisProduction of ROSEndoplasmic reticulum stress responseExpression of apoptosisCancer cell linesProstate cancerROS-dependent apoptosisChemopreventive effectsMitochondrial Nox4Mitochondrial reactive oxygen speciesReticulum stress responseTumor growthFlow cytometryMethodsThe effects
2015
Salinomycin enhances doxorubicin-induced cytotoxicity in multidrug resistant MCF-7/MDR human breast cancer cells via decreased efflux of doxorubicin
KIM K, KIM S, YU S, PARK S, CHOI H, YU H, JI J, SEO Y, AHN S. Salinomycin enhances doxorubicin-induced cytotoxicity in multidrug resistant MCF-7/MDR human breast cancer cells via decreased efflux of doxorubicin. Molecular Medicine Reports 2015, 12: 1898-1904. PMID: 25892525, PMCID: PMC4464330, DOI: 10.3892/mmr.2015.3633.Peer-Reviewed Original ResearchConceptsEfflux of doxorubicinMCF-7/MDR cellsHuman breast cancer cellsBreast cancer cellsMultidrug resistanceMDR cellsCancer cellsReverses multidrug resistanceDose-dependent mannerMDR cancer therapyResistance of doxorubicinHuman multidrug resistanceNet cellular uptakeMDR-1Chemotherapeutic agentsMRP-1Cytometric analysisProtein levelsCancer therapyDoxorubicinExpression levelsAnticancer propertiesSalinomycinPresent studyEfflux
2013
Interplay of reactive oxygen species, intracellular Ca2+ and mitochondrial homeostasis in the apoptosis of prostate cancer cells by deoxypodophyllotoxin
Kim K, Cho H, Yu S, Kim S, Yu H, Park Y, Mirkheshti N, Kim S, Song C, Chatterjee B, Ahn S. Interplay of reactive oxygen species, intracellular Ca2+ and mitochondrial homeostasis in the apoptosis of prostate cancer cells by deoxypodophyllotoxin. Journal Of Cellular Biochemistry 2013, 114: 1124-1134. PMID: 23192945, DOI: 10.1002/jcb.24455.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisBcl-2-Associated X ProteinCalciumCaspase 3Cell Line, TumorCell ProliferationCell SurvivalCytochromes cDrug Screening Assays, AntitumorDrugs, Chinese HerbalEgtazic AcidEnzyme ActivationG2 Phase Cell Cycle CheckpointsHomeostasisHumansIntracellular SpaceM Phase Cell Cycle CheckpointsMaleMembrane Potential, MitochondrialMitochondriaModels, BiologicalPodophyllotoxinProstatic NeoplasmsProtein TransportReactive Oxygen SpeciesConceptsReactive oxygen speciesMitochondrial homeostasisProstate cancer cellsCaspase-3-dependent pathwayCytochrome c releasePC-3 cell modelBax protein translocationTurn induced apoptosisCancer cellsMitochondrial membrane potentialOxygen speciesCaspase-3 activationN-acetylcysteineIncrease of ROSProtein translocationProstate cancerHuman prostate cancer cellsGeneration of ROSC releaseAntioxidant N-acetylcysteineNew anti-neoplastic agentsROS accumulationMitochondrial functionRecurrent prostate cancerLimited treatment options