2018
Salinomycin ameliorates oxidative hepatic damage through AMP-activated protein kinase, facilitating autophagy
Kim K, Lee S, Baek S, Lee E, Jang E, Lee J, Ahn S, Chang J, Oh T, Kim S, Ma J, Kim S, Park K, Kim Y. Salinomycin ameliorates oxidative hepatic damage through AMP-activated protein kinase, facilitating autophagy. Toxicology And Applied Pharmacology 2018, 360: 141-149. PMID: 30290169, DOI: 10.1016/j.taap.2018.10.002.Peer-Reviewed Original ResearchConceptsImportance of AMPKMitochondrial dysfunctionAcidic vesicle organellesOxidative stressProtein kinase activationReactive oxygen species productionProtein kinaseAMPK inhibitionKinase activationSevere oxidative stressOxygen species productionLiver injuryMolecular mechanismsIron-induced apoptosisHepatic protectantStreptomyces albusHuman diseasesAMPKCellular mechanismsLC3-IISalinomycin's effectsArachidonic acidMitochondrial impairmentROS productionAnti-cancer agents
2017
Inhibition of Autophagy Promotes Salinomycin-Induced Apoptosis via Reactive Oxygen Species-Mediated PI3K/AKT/mTOR and ERK/p38 MAPK-Dependent Signaling in Human Prostate Cancer Cells
Kim K, Park K, Kim S, Yu S, Park S, Kim Y, Seo Y, Ma J, Ahn S. Inhibition of Autophagy Promotes Salinomycin-Induced Apoptosis via Reactive Oxygen Species-Mediated PI3K/AKT/mTOR and ERK/p38 MAPK-Dependent Signaling in Human Prostate Cancer Cells. International Journal Of Molecular Sciences 2017, 18: 1088. PMID: 28524116, PMCID: PMC5454997, DOI: 10.3390/ijms18051088.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisAutophagyCell ProliferationExtracellular Signal-Regulated MAP KinasesFlavonoidsHumansImidazolesMaleMAP Kinase Signaling SystemMembrane Potential, MitochondrialP38 Mitogen-Activated Protein KinasesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsProto-Oncogene Proteins c-aktPyransPyridinesReactive Oxygen SpeciesTOR Serine-Threonine KinasesConceptsExtracellular signal-regulated kinasePI3K/Akt/mTORProstate cancer cellsAkt/mTORCancer cellsP38 MAPK-dependent signalingUpregulation of ERKSignal-regulated kinaseMAPK-dependent signalingP38 MAPK signaling pathwaysMitochondrial membrane potentialMAPK signaling pathwaysChemo-resistant cancersHuman prostate cancer cellsReactive oxygen species productionInhibition of autophagyPI3K inhibitorsPotential antitumor mechanismAcridine orange stainingCaspase-3 activityOxygen species productionSignaling pathwaysP38 inhibitorPropidium iodide assayMTOR activitySalinomycin induces endoplasmic reticulum stress-mediated autophagy and apoptosis through generation of reactive oxygen species in human glioma U87MG cells
Yu S, Kim S, Kim K, Ji J, Seo Y, Yu H, Ahn S. Salinomycin induces endoplasmic reticulum stress-mediated autophagy and apoptosis through generation of reactive oxygen species in human glioma U87MG cells. Oncology Reports 2017, 37: 3321-3328. PMID: 28498472, DOI: 10.3892/or.2017.5615.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisAutophagyCell Line, TumorCell ProliferationEndoplasmic ReticulumEndoplasmic Reticulum StressGliomaHumansPyransReactive Oxygen SpeciesConceptsReactive oxygen speciesER stressHuman glioma U87MG cellsEndoplasmic reticulumGlioma U87MG cellsU87MG cellsStress-related proteinsAcidic vesicular organellesOxygen speciesER stress-related proteinsHuman cancer cellsAutophagy marker proteinsVesicular organellesImpact of salinomycinEndoplasmic reticulum stress-mediated autophagyMolecular mechanismsCell deathAutophagyMarker proteinsAutophagy inhibitorCancer cell linesGlioma cancer cell linesInduced apoptosisROS scavengerExpression levelsDeoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells
Kim S, Son K, Kim K, Yu S, Park S, Kim Y, Nam H, Suh J, Ji J, Ahn S. Deoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells. Pharmacological Reports 2017, 69: 878-884. PMID: 28623712, DOI: 10.1016/j.pharep.2017.04.007.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic Agents, PhytogenicApoptosisAutophagyBone NeoplasmsCell Line, TumorCell SurvivalDrugs, Chinese HerbalGene Expression RegulationHumansOsteosarcomaPhosphoinositide-3 Kinase InhibitorsPodophyllotoxinProto-Oncogene Proteins c-aktReactive Oxygen SpeciesTOR Serine-Threonine KinasesConceptsReactive oxygen speciesPI3K/AKT/mTOR pathwayAKT/mTOR pathwayU2OS cellsAcidic vesicular organelles (AVOs) formationMTOR pathwayMitochondrial reactive oxygen speciesOsteosarcoma U2OS cellsCell death modeOxygen speciesPropidium iodidePI3K/Akt/mTORCell cycle arrestLight chain 3Cell viabilityCellular processesInhibition of apoptosisAkt/mTOROrganelle formationAcridine orange stainingAutophagy processApoptotic responseDeath modeWestern blot analysisCell survivalSalinomycin Induces Reactive Oxygen Species and Apoptosis in Aggressive Breast Cancer Cells as Mediated with Regulation of Autophagy
Kim K, Park K, Kim S, Yu S, Lee D, Kim Y, Noh K, YEUL J, Seo Y, Ahn S. Salinomycin Induces Reactive Oxygen Species and Apoptosis in Aggressive Breast Cancer Cells as Mediated with Regulation of Autophagy. Anticancer Research 2017, 37: 1747-1758. PMID: 28373437, DOI: 10.21873/anticanres.11507.Peer-Reviewed Original ResearchConceptsMDA-MB-231 cellsAggressive breast cancer cellsBreast cancer cellsCancer cellsReactive oxygen speciesBreast cancer cell linesROS productionMitochondrial membrane potentialScavenger of ROSInduces reactive oxygen speciesCancer cell linesCaspase-3/9 activityPropidium iodide stainingMCF-7 cellsOxygen speciesAcceleration of apoptosisAcridine orange stainingAutophagy inhibitionMonocarboxylic ionophoreChemotherapeutic drugsCancer treatmentMitochondrial dysfunctionIodide stainingRegulation of autophagyUnderlying mechanismLasalocid induces cytotoxic apoptosis and cytoprotective autophagy through reactive oxygen species in human prostate cancer PC-3 cells
Kim K, Kim S, Yu S, Park S, Kim Y, Nam H, An H, Yu H, Kim Y, Ji J, Seo Y, Ahn S. Lasalocid induces cytotoxic apoptosis and cytoprotective autophagy through reactive oxygen species in human prostate cancer PC-3 cells. Biomedicine & Pharmacotherapy 2017, 88: 1016-1024. PMID: 28178613, DOI: 10.1016/j.biopha.2017.01.140.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisAutophagyCell Line, TumorCell ProliferationG1 Phase Cell Cycle CheckpointsHumansLasalocidMaleProstatic NeoplasmsReactive Oxygen SpeciesConceptsProstate cancer cellsHuman prostate cancer cellsAcidic vesicular organelles (AVOs) formationApoptotic cell death pathwayCancer cellsCell death pathwaysProtein 1 light chain 3Cell cycle arrestLight chain 3Human prostate cancer PC-3 cellsProstate cancer PC-3 cellsReactive oxygen species productionPossible signal pathwaysCancer PC-3 cellsInhibition of autophagyOrganelle formationDeath pathwaysNew potential chemotherapeutic agentsReactive oxygen speciesOxygen species productionMitochondrial hyperpolarizationMolecular mechanismsPotential chemotherapeutic agentProduction of ROSCytoprotective autophagy
2016
Monensin Induces PC-3 Prostate Cancer Cell Apoptosis via ROS Production and Ca2+ Homeostasis Disruption
KIM S, KIM K, YU S, PARK S, YU H, SEO Y, AHN S. Monensin Induces PC-3 Prostate Cancer Cell Apoptosis via ROS Production and Ca2+ Homeostasis Disruption. Anticancer Research 2016, 36: 5835-5843. PMID: 27793906, DOI: 10.21873/anticanres.11168.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCalciumCell Line, TumorCell ProliferationHomeostasisHumansMaleMonensinProstatic NeoplasmsReactive Oxygen SpeciesConceptsCell cycle arrestApoptosis-related proteinsCell cycleCancer cellsPoly ADP-ribose polymerase (PARP) cleavageADP-ribose polymerase cleavageDisruption of CaProstate cancer cell apoptosisRibose polymerase cleavagePhase cell cycle arrestHuman prostate cancer cellsCell viabilityReactive oxygen species productionCancer cell apoptosisAnticancer effectsOxygen species productionMitochondrial ROS inhibitorProduction of ROSMitochondrial ROSDependent apoptosisProstate cancer cellsProstate cell linesPolymerase cleavageCell growth inhibitionPC-3 cellsToyocamycin induces apoptosis via the crosstalk between reactive oxygen species and p38/ERK MAPKs signaling pathway in human prostate cancer PC-3 cells
Park S, Kim S, Kim K, Yu S, Choi H, Kim Y, Nam H, Seo Y, Ahn S. Toyocamycin induces apoptosis via the crosstalk between reactive oxygen species and p38/ERK MAPKs signaling pathway in human prostate cancer PC-3 cells. Pharmacological Reports 2016, 69: 90-96. PMID: 27912102, DOI: 10.1016/j.pharep.2016.10.014.Peer-Reviewed Original ResearchConceptsProstate cancer PC-3 cellsPC-3 cellsCancer PC-3 cellsOxygen species productionROS productionERK MAPKNon-malignant RWPE-1 cellsExtracellular signal-regulated kinase (ERK) activityMitochondrial dysfunctionSpecies productionMitochondrial membrane potentialCell cycle arrestHuman prostate cancer PC-3 cellsReactive oxygen species productionCell viabilityRWPE-1 cellsProtein kinaseStreptomyces speciesKinase activityReactive oxygen speciesERK activationMolecular mechanismsP38 activationMAPK proteinsApoptotic effectsSilibinin induces mitochondrial NOX4-mediated endoplasmic reticulum stress response and its subsequent apoptosis
Kim S, Kim K, Yu S, Seo Y, Chun S, Yu H, Ahn S. Silibinin induces mitochondrial NOX4-mediated endoplasmic reticulum stress response and its subsequent apoptosis. BMC Cancer 2016, 16: 452. PMID: 27405931, PMCID: PMC4942927, DOI: 10.1186/s12885-016-2516-6.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesNOX4 expressionDisruption of Ca2ER stress responseProstate cancer PC-3 cellsCancer PC-3 cellsRegulation of NOX4Inhibited tumor growthCell linesPC-3 cellsProstate cell linesSilibinin-induced apoptosisProduction of ROSEndoplasmic reticulum stress responseExpression of apoptosisCancer cell linesProstate cancerROS-dependent apoptosisChemopreventive effectsMitochondrial Nox4Mitochondrial reactive oxygen speciesReticulum stress responseTumor growthFlow cytometryMethodsThe effectsSalinomycin simultaneously induces apoptosis and autophagy through generation of reactive oxygen species in osteosarcoma U2OS cells
Kim S, Choi Y, Kim K, Yu S, Seo Y, Chun S, Noh K, Suh J, Ahn S. Salinomycin simultaneously induces apoptosis and autophagy through generation of reactive oxygen species in osteosarcoma U2OS cells. Biochemical And Biophysical Research Communications 2016, 473: 607-613. PMID: 27033598, DOI: 10.1016/j.bbrc.2016.03.132.Peer-Reviewed Original ResearchMeSH KeywordsAntibiotics, AntineoplasticApoptosisAutophagyBone NeoplasmsCell Line, TumorHumansIonophoresOsteosarcomaPyransReactive Oxygen SpeciesConceptsAcidic vesicular organellesReactive oxygen speciesMitochondrial membrane potentialChange of MMPOsteosarcoma U2OS cellsInhibition of autophagyU2OS cellsExpression of apoptosisCancer cell linesOxygen speciesFlow cytometryWestern blottingCaspase-3Cancer therapyVesicular organellesPoly (ADP-ribose) polymerase
2015
Autophagy inhibition enhances silibinin-induced apoptosis by regulating reactive oxygen species production in human prostate cancer PC-3 cells
Kim S, Kim K, Yu S, Park S, Choi H, Ji J, Ahn S. Autophagy inhibition enhances silibinin-induced apoptosis by regulating reactive oxygen species production in human prostate cancer PC-3 cells. Biochemical And Biophysical Research Communications 2015, 468: 151-156. PMID: 26522224, DOI: 10.1016/j.bbrc.2015.10.143.Peer-Reviewed Original ResearchMeSH KeywordsAntioxidantsApoptosisAutophagyCell Line, TumorHumansMaleMicrotubule-Associated ProteinsMilk ThistleProstateProstatic NeoplasmsReactive Oxygen SpeciesSilybinSilymarinConceptsPC-3 cellsProstate cancer PC-3 cellsCancer PC-3 cellsAcidic vesicular organellesSilibinin-induced apoptosisReactive oxygen speciesAnticancer effectsHuman prostate cancer PC-3 cellsMicrotubule-associated protein 1 light chain 3Inhibition of ROSProtein 1 light chain 3Major bioactive componentsInhibition of autophagyReactive oxygen species productionCancer cell linesLight chain 3Supportive therapyLiver conditionsOxygen species productionCancer modelProtective roleSilibininSilibinin-induced autophagyROS inhibitorChain 3
2013
Interplay of reactive oxygen species, intracellular Ca2+ and mitochondrial homeostasis in the apoptosis of prostate cancer cells by deoxypodophyllotoxin
Kim K, Cho H, Yu S, Kim S, Yu H, Park Y, Mirkheshti N, Kim S, Song C, Chatterjee B, Ahn S. Interplay of reactive oxygen species, intracellular Ca2+ and mitochondrial homeostasis in the apoptosis of prostate cancer cells by deoxypodophyllotoxin. Journal Of Cellular Biochemistry 2013, 114: 1124-1134. PMID: 23192945, DOI: 10.1002/jcb.24455.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisBcl-2-Associated X ProteinCalciumCaspase 3Cell Line, TumorCell ProliferationCell SurvivalCytochromes cDrug Screening Assays, AntitumorDrugs, Chinese HerbalEgtazic AcidEnzyme ActivationG2 Phase Cell Cycle CheckpointsHomeostasisHumansIntracellular SpaceM Phase Cell Cycle CheckpointsMaleMembrane Potential, MitochondrialMitochondriaModels, BiologicalPodophyllotoxinProstatic NeoplasmsProtein TransportReactive Oxygen SpeciesConceptsReactive oxygen speciesMitochondrial homeostasisProstate cancer cellsCaspase-3-dependent pathwayCytochrome c releasePC-3 cell modelBax protein translocationTurn induced apoptosisCancer cellsMitochondrial membrane potentialOxygen speciesCaspase-3 activationN-acetylcysteineIncrease of ROSProtein translocationProstate cancerHuman prostate cancer cellsGeneration of ROSC releaseAntioxidant N-acetylcysteineNew anti-neoplastic agentsROS accumulationMitochondrial functionRecurrent prostate cancerLimited treatment options
2012
Pipernonaline from Piper longum Linn. induces ROS-mediated apoptosis in human prostate cancer PC-3 cells
Lee W, Kim K, Yu S, Kim S, Chun S, Ji J, Yu H, Ahn S. Pipernonaline from Piper longum Linn. induces ROS-mediated apoptosis in human prostate cancer PC-3 cells. Biochemical And Biophysical Research Communications 2012, 430: 406-412. PMID: 23159637, DOI: 10.1016/j.bbrc.2012.11.030.Peer-Reviewed Original ResearchConceptsPC-3 cellsHuman prostate cancer PC-3 cellsProstate cancer PC-3 cellsCancer PC-3 cellsReactive oxygen speciesAndrogen-independent PC-3LNCaP prostate cellsCaspase-3 assay kitHuman prostate cancerProstate cancer cellsIncrease of intracellularInduces reactive oxygen speciesTime-dependent mannerProstate cancerPiper longum LinnCleavage of PARPProstate cellsN-acetylcysteineAntiproliferation effectCyclin D1Caspase-3 activationAssay KitBcl-2 proteinCandidate agentCancer cells