2001
ETB receptor activation causes exocytic insertion of NHE3 in OKP cells
Peng Y, Amemiya M, Yang X, Fan L, Moe O, Yin H, Preisig P, Yanagisawa M, Alpern R. ETB receptor activation causes exocytic insertion of NHE3 in OKP cells. American Journal Of Physiology. Renal Physiology 2001, 280: f34-f42. PMID: 11133512, DOI: 10.1152/ajprenal.2001.280.1.f34.Peer-Reviewed Original ResearchConceptsEndothelin-1Sodium/hydrogen exchanger 3ET-1-induced increaseTotal cellular NHE3NHE3 activityNET traffickingTwo- to threefold increaseApical membrane NHE3OKP cellsMaximal effectReceptor activationClone PApical NHE3Exchanger 3NHE3Exocytic insertionThreefold increaseApical membraneCytochalasin D
1999
ETB receptor activation leads to activation and phosphorylation of NHE3
Peng Y, Moe OW, Chu T, Preisig PA, Yanagisawa M, Alpern RJ. ETB receptor activation leads to activation and phosphorylation of NHE3. American Journal Of Physiology 1999, 276: c938-c945. PMID: 10199826, DOI: 10.1152/ajpcell.1999.276.4.c938.Peer-Reviewed Original Research
1995
Overexpression of csk inhibits acid-induced activation of NHE-3.
Yamaji Y, Amemiya M, Cano A, Preisig PA, Miller RT, Moe OW, Alpern RJ. Overexpression of csk inhibits acid-induced activation of NHE-3. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6274-6278. PMID: 7541536, PMCID: PMC41500, DOI: 10.1073/pnas.92.14.6274.Peer-Reviewed Original Research1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAnimalsBenzoquinonesCell LineChickensCSK Tyrosine-Protein KinaseGene ExpressionGene Expression RegulationHydrogen-Ion ConcentrationIsoquinolinesKidneyKineticsLactams, MacrocyclicOpossumsPiperazinesProtein Kinase InhibitorsProtein-Tyrosine KinasesProto-Oncogene Proteins pp60(c-src)QuinonesRecombinant ProteinsRifabutinSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSrc-Family KinasesTetradecanoylphorbol AcetateTransfectionInvolvement of pRB family in TGF beta-dependent epithelial cell hypertrophy.
Franch HA, Shay JW, Alpern RJ, Preisig PA. Involvement of pRB family in TGF beta-dependent epithelial cell hypertrophy. Journal Of Cell Biology 1995, 129: 245-254. PMID: 7698989, PMCID: PMC2120382, DOI: 10.1083/jcb.129.1.245.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, Polyomavirus TransformingBlotting, NorthernCell CycleCell DivisionCell LineCells, CulturedEpidermal Growth FactorEpithelial CellsEpitheliumGene ExpressionHumansHypertrophyKidneyKidney TubulesKineticsOncogene Proteins, ViralPapillomavirus E7 ProteinsPhosphorylationProto-Oncogene Proteins c-fosRabbitsRatsRecombinant ProteinsRepressor ProteinsRetinoblastoma ProteinRNA, MessengerTransfectionTransforming Growth Factor betaTumor Suppressor Protein p53ConceptsEpithelial cell hypertrophyNRK-52E cellsCell hypertrophyHPV16 E6TGF betaSV40 large T antigenC-fos mRNA abundanceGrowth factor beta 1SV40 large T antigen expressionLarge T antigenLarge T antigen expressionMechanisms of hypertrophyRenal cell hypertrophyRabbit proximal tubuleActive pRbT-antigen expressionRenal functionT antigenRenal hypertrophyAntigen expressionEpidermal growth factorEGF-induced increaseProximal tubulesHypertrophyHPV16 E7
1993
Cyclic adenosine monophosphate acutely inhibits and chronically stimulates Na/H antiporter in OKP cells.
Cano A, Preisig P, Alpern RJ. Cyclic adenosine monophosphate acutely inhibits and chronically stimulates Na/H antiporter in OKP cells. Journal Of Clinical Investigation 1993, 92: 1632-1638. PMID: 7691881, PMCID: PMC288321, DOI: 10.1172/jci116748.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityRenal Na excretionNa excretionNa/H antiporterOKP cellsH antiporterAntiporter activityAddition of forskolinParathyroid hormoneAngiotensin IIAcute effectsChronic increaseChronic activationCyclic adenosine monophosphateChronic effectsChronic applicationNonspecific effectsPersistent activationPersistent increaseAlpha-adrenergic catecholaminesCell cAMPEndogenous generationAdenosine monophosphateH exposureCell acidificationRenin expression in renal proximal tubule.
Moe OW, Ujiie K, Star RA, Miller RT, Widell J, Alpern RJ, Henrich WL. Renin expression in renal proximal tubule. Journal Of Clinical Investigation 1993, 91: 774-779. PMID: 7680667, PMCID: PMC288027, DOI: 10.1172/jci116296.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, SouthernCells, CulturedCloning, MolecularColforsinEnalaprilIn Vitro TechniquesIsoproterenolKidney Tubules, ProximalKineticsMaleMolecular Sequence DataMuscle, Smooth, VascularOligodeoxyribonucleotidesPoly APolymerase Chain ReactionRabbitsRatsRats, Sprague-DawleyReninRNARNA, MessengerTranscription, GeneticConceptsProximal tubule cellsCultured proximal tubule cellsRenin-angiotensin systemProximal tubulesRenal proximal tubulesTubule cellsRenin activityLocal renin-angiotensin systemPrimary culturesPresence of reninRabbit proximal tubule cellsAngiotensin-converting enzymeMammalian renal proximal tubulePrevious immunohistochemical studiesSynthesized reninAngiotensin receptorsAngiotensin IIRenin contentPolymerase chain reactionRenin expressionImmunohistochemical studyM isoproterenolM forskolinNormal ratsRenin mRNA
1992
Role of protein kinase C and transcription factor AP-1 in the acid-induced increase in Na/H antiporter activity.
Horie S, Moe O, Yamaji Y, Cano A, Miller RT, Alpern RJ. Role of protein kinase C and transcription factor AP-1 in the acid-induced increase in Na/H antiporter activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 5236-5240. PMID: 1319056, PMCID: PMC49266, DOI: 10.1073/pnas.89.12.5236.Peer-Reviewed Original ResearchAnimalsCarrier ProteinsCell Line, TransformedChloramphenicol O-AcetyltransferaseGenes, fosGenes, junGlyceraldehyde-3-Phosphate DehydrogenasesHydrogen-Ion ConcentrationKidney Tubules, ProximalKineticsMiceProtein Kinase CProto-Oncogene Proteins c-junRNA, MessengerSimian virus 40Sodium-Hydrogen ExchangersSphingosineTetradecanoylphorbol AcetateTransfection
1991
Chronic K depletion stimulates rat renal brush-border membrane Na-citrate cotransporter
Levi M, McDonald LA, Preisig PA, Alpern RJ. Chronic K depletion stimulates rat renal brush-border membrane Na-citrate cotransporter. American Journal Of Physiology 1991, 261: f767-f773. PMID: 1683169, DOI: 10.1152/ajprenal.1991.261.5.f767.Peer-Reviewed Original ResearchEffects of endothelin on in vitro renin secretion
Moe O, Tejedor A, Campbell WB, Alpern RJ, Henrich WL. Effects of endothelin on in vitro renin secretion. American Journal Of Physiology 1991, 260: e521-e525. PMID: 2018117, DOI: 10.1152/ajpendo.1991.260.4.e521.Peer-Reviewed Original ResearchConceptsRat kidney cortical slicesRenin secretionKidney cortical slicesEndothelin-1Juxtaglomerular cellsRenin releaseCortical slicesRenin inhibitionBasal renin releaseVitro renin secretionBasal renin secretionRenal renin secretionEffects of endothelinIntracellular calcium bufferAddition of isoproterenolMechanism of actionRenin inhibitorsIsoproterenolSecretionCalcium bufferDistinct preparationsBAPTASimilar effectsDirect effectInhibitionIncreased Na/H antiporter and Na/3HCO3 symporter activities in chronic hyperfiltration. A model of cell hypertrophy.
Preisig PA, Alpern RJ. Increased Na/H antiporter and Na/3HCO3 symporter activities in chronic hyperfiltration. A model of cell hypertrophy. The Journal Of General Physiology 1991, 97: 195-217. PMID: 1849958, PMCID: PMC2216475, DOI: 10.1085/jgp.97.2.195.Peer-Reviewed Original ResearchConceptsHyperfiltration groupChronic hyperfiltrationNa/H antiporterKidney weightCell hypertrophyHigher glomerular filtration rateNa/H antiporter activityH antiporterGlomerular filtration rateVivo microperfused rat proximal tubuleCell pHRat proximal tubuleSymporter activityFiltration rateProximal tubulesUltrafiltrate-like solutionHyperfiltrationSteady-state cell pHProtein feedingPeritubular perfusateDietary proteinParallel increaseUninephrectomyHypertrophyCell acidificationDietary NaCl modulates Na(+)-H+ antiporter activity in renal cortical apical membrane vesicles
Moe OW, Tejedor A, Levi M, Seldin DW, Preisig PA, Alpern RJ. Dietary NaCl modulates Na(+)-H+ antiporter activity in renal cortical apical membrane vesicles. American Journal Of Physiology 1991, 260: f130-f137. PMID: 1847012, DOI: 10.1152/ajprenal.1991.260.1.f130.Peer-Reviewed Original ResearchConceptsDietary NaClNaCl reabsorptionSystemic acid-base disturbancesExtracellular fluid volume depletionNaHCO3 reabsorptionExtracellular fluid volume expansionFluid volume depletionHigh-salt dietChronic metabolic alkalosisFluid volume expansionAcridine orange fluorescence methodExtracellular fluid volumeAcid-base disturbancesAntiporter activityDietary protocolChronic alterationsVolume depletionMetabolic alkalosisChronic changesHS dietApical membrane vesiclesLS dietFluid volumeRatsReabsorption
1990
Preincubation in acid medium increases Na/H antiporter activity in cultured renal proximal tubule cells.
Horie S, Moe O, Tejedor A, Alpern RJ. Preincubation in acid medium increases Na/H antiporter activity in cultured renal proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 4742-4745. PMID: 2162055, PMCID: PMC54193, DOI: 10.1073/pnas.87.12.4742.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityProximal tubule cellsTubule cellsCultured renal proximal tubule cellsCultured proximal tubule cellsRenal proximal tubule cellsPreincubation of fibroblastsExtracellular fluidAntiporter activityChronic acidosisAcidotic environmentCO2/HCO3Small inhibitionPreincubationPreincubation mediumAddition of cycloheximideCell acidificationAcid preincubationPresent studyProtein synthesisCellsAcidosisActivityEpithelium
1987
Kinetic transport model for cellular regulation of pH and solute concentration in the renal proximal tubule
Verkman AS, Alpern RJ. Kinetic transport model for cellular regulation of pH and solute concentration in the renal proximal tubule. Biophysical Journal 1987, 51: 533-546. PMID: 3580482, PMCID: PMC1329926, DOI: 10.1016/s0006-3495(87)83379-9.Peer-Reviewed Original Research
1986
Electrogenic sodium/bicarbonate cotransport in rabbit renal cortical basolateral membrane vesicles.
Akiba T, Alpern RJ, Eveloff J, Calamina J, Warnock DG. Electrogenic sodium/bicarbonate cotransport in rabbit renal cortical basolateral membrane vesicles. Journal Of Clinical Investigation 1986, 78: 1472-1478. PMID: 3782468, PMCID: PMC423898, DOI: 10.1172/jci112738.Peer-Reviewed Original ResearchConceptsMembrane vesiclesBasolateral membrane vesiclesSodium uptakePotassium diffusion potentialBicarbonate cotransporterSaturable stimulationSodium/bicarbonate cotransporterVesiclesRabbit renal cortexProteinDisulfonic stilbeneBicarbonate transportPresence of bicarbonateUptakeKinetic analysisRenal cortex
1984
Bicarbonate-water interactions in the rat proximal convoluted tubule. An effect of volume flux on active proton secretion.
Alpern RJ. Bicarbonate-water interactions in the rat proximal convoluted tubule. An effect of volume flux on active proton secretion. The Journal Of General Physiology 1984, 84: 753-770. PMID: 6096481, PMCID: PMC2228763, DOI: 10.1085/jgp.84.5.753.Peer-Reviewed Original ResearchIncreased Vmax for Na+/H+ antiporter activity in proximal tubule brush border vesicles from rabbits with metabolic acidosis
Tsai CJ, Ives HE, Alpern RJ, Yee VJ, Warnock DG, Rector FC. Increased Vmax for Na+/H+ antiporter activity in proximal tubule brush border vesicles from rabbits with metabolic acidosis. American Journal Of Physiology 1984, 247: f339-f343. PMID: 6087681, DOI: 10.1152/ajprenal.1984.247.2.f339.Peer-Reviewed Original Research
1983
Flow dependence of proximal tubular bicarbonate absorption
Alpern RJ, Cogan MG, Rector FC. Flow dependence of proximal tubular bicarbonate absorption. American Journal Of Physiology 1983, 245: f478-f484. PMID: 6414312, DOI: 10.1152/ajprenal.1983.245.4.f478.Peer-Reviewed Original ResearchConceptsLuminal bicarbonate concentrationBicarbonate absorptionFlow-dependent increaseFlow-dependent decreaseProximal convoluted tubulesBicarbonate concentrationFlow-induced increaseFlow-dependent changesConvoluted tubulesX minPmol xStimulationProton secretionTubulesPerfusion rateMaximal rateObserved stimulationMin
1982
Effect of luminal bicarbonate concentration on proximal acidification in the rat
Alpern RJ, Cogan MG, Rector FC. Effect of luminal bicarbonate concentration on proximal acidification in the rat. American Journal Of Physiology 1982, 243: f53-f59. PMID: 7091367, DOI: 10.1152/ajprenal.1982.243.1.f53.Peer-Reviewed Original ResearchConceptsLuminal bicarbonate concentrationBicarbonate absorptionBicarbonate concentrationNet bicarbonate absorptionProximal convoluted tubulesProximal tubular acidificationProton secretionBicarbonate leakProximal acidificationTubular acidificationConvoluted tubulesBicarbonate permeabilitySecretionRats