2010
Acid regulation of NaDC-1 requires a functional endothelin B receptor
Liu L, Zacchia M, Tian X, Wan L, Sakamoto A, Yanagisawa M, Alpern RJ, Preisig PA. Acid regulation of NaDC-1 requires a functional endothelin B receptor. Kidney International 2010, 78: 895-904. PMID: 20703215, DOI: 10.1038/ki.2010.264.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAnimalsBiological TransportCell LineDicarboxylic Acid TransportersDisease Models, AnimalEndothelin B Receptor AntagonistsEndothelin-1Hydrogen-Ion ConcentrationKidneyMiceMice, KnockoutMicrovilliOligopeptidesOpossumsOrganic Anion Transporters, Sodium-DependentPiperidinesProtein Structure, TertiaryReceptor, Endothelin AReceptor, Endothelin BRecombinant Fusion ProteinsSignal TransductionSymportersTime FactorsTransfectionConceptsEndothelin-1Endothelin-B receptor antagonistFunctional endothelin B receptorsStimulatory effectAcid-stimulatory effectET-1 effectsB receptor antagonistNHE3 activityEndothelin B receptorWild-type miceET-1 stimulationMajor physiological stimulusNaDC-1Acid ingestionReceptor antagonistCitrate reabsorptionReceptor expressionCitrate excretionOpossum kidneyAcid loadB receptorAcid feedingPhysiological stimuliStimulationMice
2002
Glucagon Acutely Inhibits but Chronically Activates Na+/H+ Antiporter 3 Activity in OKP Cells
Amemiya M, Kusano E, Muto S, Tabei K, Ando Y, Alpern RJ, Asano Y. Glucagon Acutely Inhibits but Chronically Activates Na+/H+ Antiporter 3 Activity in OKP Cells. Experimental Nephrology 2002, 10: 26-33. PMID: 11803202, DOI: 10.1159/000049895.Peer-Reviewed Original ResearchConceptsNHE3 protein abundanceOKP cellsNHE3 activityCell pH recoveryRat renal proximal tubulesDibutyryl cAMPThick ascending limbRenal proximal tubulesWestern blot analysisM dibutyryl-cAMPChronic activationPKA-dependent pathwayProximal tubulesAscending limbGlucagonSurface membraneAcid loadNHE3 mRNAProtein abundancePH recoveryExchanger 3Independent mechanismsSignificant increaseBlot analysisP cells
1999
Glucocorticoids enhance acid activation of the Na+/H+ exchanger 3 (NHE3)
Ambühl P, Yang X, Peng Y, Preisig P, Moe O, Alpern R. Glucocorticoids enhance acid activation of the Na+/H+ exchanger 3 (NHE3). Journal Of Clinical Investigation 1999, 103: 429-435. PMID: 9927505, PMCID: PMC407891, DOI: 10.1172/jci2913.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCell LineGlucocorticoidsHydrogen-Ion ConcentrationSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNHE3 protein abundanceM hydrocortisoneNHE3 activityAcid incubationNHE3 mRNA abundanceTrafficking of NHE3Absence of hydrocortisoneOpossum kidney cellsExogenous glucocorticoidsExchanger 3 (NHE3) activityHydrocortisoneProtein abundanceGlucocorticoidsProtein synthesisExchanger 3Kidney cellsTwofold increaseMRNA abundanceApical membrane
1997
Chronic metabolic acidosis enhances NHE-3 protein abundance and transport activity in the rat thick ascending limb by increasing NHE-3 mRNA.
Laghmani K, Borensztein P, Ambühl P, Froissart M, Bichara M, Moe OW, Alpern RJ, Paillard M. Chronic metabolic acidosis enhances NHE-3 protein abundance and transport activity in the rat thick ascending limb by increasing NHE-3 mRNA. Journal Of Clinical Investigation 1997, 99: 24-30. PMID: 9011571, PMCID: PMC507763, DOI: 10.1172/jci119128.Peer-Reviewed Original Research
1996
Endothelin(B) receptor activates NHE-3 by a Ca2+-dependent pathway in OKP cells.
Chu TS, Peng Y, Cano A, Yanagisawa M, Alpern RJ. Endothelin(B) receptor activates NHE-3 by a Ca2+-dependent pathway in OKP cells. Journal Of Clinical Investigation 1996, 97: 1454-1462. PMID: 8617878, PMCID: PMC507205, DOI: 10.1172/jci118567.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityReceptor blockersET-1OKP cellsET-1-induced increaseEndothelin receptor blockersNHE-3Pertussis toxin pretreatmentSelective receptor blockerAntiporter activationM endothelinPertussis toxinToxin pretreatmentSustained increaseAntiporter isoformsEndothelinTransient increaseAntiporter activityBlockersKinase inhibitorsCyclic GMPAdenylyl cyclaseReceptor cDNADependent pathwayReceptors
1991
Basolateral membrane H/HCO3 transport in renal tubules
Preisig P, Alpern R. Basolateral membrane H/HCO3 transport in renal tubules. Kidney International 1991, 39: 1077-1086. PMID: 1895662, DOI: 10.1038/ki.1991.137.Peer-Reviewed Original ResearchMeSH KeywordsAcidsAnimalsBicarbonatesBiological TransportHumansHydrogenKidneyKidney TubulesMembranesConceptsCell exitRegulation of proximal reabsorption by effective arterial blood volume.
Seldin DW, Preisig PA, Alpern RJ. Regulation of proximal reabsorption by effective arterial blood volume. Seminars In Nephrology 1991, 11: 212-9. PMID: 2034926.Peer-Reviewed Original ResearchConceptsEffective arterial blood volumeEffective arterial volumeRenal nerve activityProximal tubular transportAtrial natriuretic factorArterial blood volumeLevels of hormonesRenal hemodynamicsNerve activityDietary NaClAngiotensin IIProximal reabsorptionNatriuretic factorBlood volumeTubular transportProximal tubulesChronic effectsArterial volumeOncotic pressureNorepinephrineHemodynamicsEpinephrineHormoneReabsorptionDietary NaCl modulates Na(+)-H+ antiporter activity in renal cortical apical membrane vesicles
Moe OW, Tejedor A, Levi M, Seldin DW, Preisig PA, Alpern RJ. Dietary NaCl modulates Na(+)-H+ antiporter activity in renal cortical apical membrane vesicles. American Journal Of Physiology 1991, 260: f130-f137. PMID: 1847012, DOI: 10.1152/ajprenal.1991.260.1.f130.Peer-Reviewed Original ResearchConceptsDietary NaClNaCl reabsorptionSystemic acid-base disturbancesExtracellular fluid volume depletionNaHCO3 reabsorptionExtracellular fluid volume expansionFluid volume depletionHigh-salt dietChronic metabolic alkalosisFluid volume expansionAcridine orange fluorescence methodExtracellular fluid volumeAcid-base disturbancesAntiporter activityDietary protocolChronic alterationsVolume depletionMetabolic alkalosisChronic changesHS dietApical membrane vesiclesLS dietFluid volumeRatsReabsorption
1990
Pathways for apical and basolateral membrane NH3 and NH4+ movement in rat proximal tubule
Preisig PA, Alpern RJ. Pathways for apical and basolateral membrane NH3 and NH4+ movement in rat proximal tubule. American Journal Of Physiology 1990, 259: f587-f593. PMID: 2221097, DOI: 10.1152/ajprenal.1990.259.4.f587.Peer-Reviewed Original ResearchBasolateral membrane H/OH/HCO3 transport mechanisms along the nephron: role in control of pHi and transepithelial bicarbonate transport.
Preisig PA, Hays SR, Alpern RJ. Basolateral membrane H/OH/HCO3 transport mechanisms along the nephron: role in control of pHi and transepithelial bicarbonate transport. Seminars In Nephrology 1990, 10: 104-14. PMID: 2156327.Peer-Reviewed Original Research
1989
Basolateral membrane H-OH-HCO3 transport in the proximal tubule
Preisig P, Alpern R. Basolateral membrane H-OH-HCO3 transport in the proximal tubule. American Journal Of Physiology 1989, 256: f751-f765. PMID: 2541627, DOI: 10.1152/ajprenal.1989.256.5.f751.Peer-Reviewed Original ResearchConceptsProximal tubulesS3 proximal tubuleChronic respiratory acidosisChronic metabolic acidosisRabbit proximal tubuleChronic hyperfiltrationMetabolic acidosisNa-H antiporterRespiratory acidosisCl-HCO3 exchangerCl absorptionGreater transport capacityAlkalosisAcidosisTubulesTransporter activityMembrane mechanismsBasolateral membraneCotransporterHyperfiltrationRatsEpithelium
1987
Basolateral membrane Na/base cotransport is dependent on CO2/HCO3 in the proximal convoluted tubule.
Krapf R, Alpern RJ, Rector FC, Berry CA. Basolateral membrane Na/base cotransport is dependent on CO2/HCO3 in the proximal convoluted tubule. The Journal Of General Physiology 1987, 90: 833-853. PMID: 2831294, PMCID: PMC2228880, DOI: 10.1085/jgp.90.6.833.Peer-Reviewed Original ResearchBasolateral membrane Cl/HCO3 exchange in the rat proximal convoluted tubule. Na-dependent and -independent modes.
Alpern RJ, Chambers M. Basolateral membrane Cl/HCO3 exchange in the rat proximal convoluted tubule. Na-dependent and -independent modes. The Journal Of General Physiology 1987, 89: 581-598. PMID: 2953859, PMCID: PMC2215917, DOI: 10.1085/jgp.89.4.581.Peer-Reviewed Original ResearchConceptsCl/HCO3 exchangeNa-free solutionHCO3- exchangePeritubular NaCell pHHCO3- transport mechanismsBasolateral membraneProximal tubulesCoupling of NaMammalian proximal tubuleMeq/Similar increaseAmbient ClExtracellular fluidTubulesNa dependenceCotransporterNa concentrationCl concentrationsCellsSimilar amountsKinetic transport model for cellular regulation of pH and solute concentration in the renal proximal tubule
Verkman AS, Alpern RJ. Kinetic transport model for cellular regulation of pH and solute concentration in the renal proximal tubule. Biophysical Journal 1987, 51: 533-546. PMID: 3580482, PMCID: PMC1329926, DOI: 10.1016/s0006-3495(87)83379-9.Peer-Reviewed Original ResearchCarbonic Anhydrase Inhibitors
Preisig PA, Toto RD, Alpern RJ. Carbonic Anhydrase Inhibitors. Renal Physiology 1987, 10: 136-159. PMID: 3133728, DOI: 10.1159/000173126.Peer-Reviewed Original ResearchMeSH KeywordsBicarbonatesBiological TransportCarbonic Anhydrase InhibitorsCarbonic AnhydrasesChloridesDiuresisHumansKidneyKidney Concentrating Ability
1986
Electrogenic sodium/bicarbonate cotransport in rabbit renal cortical basolateral membrane vesicles.
Akiba T, Alpern RJ, Eveloff J, Calamina J, Warnock DG. Electrogenic sodium/bicarbonate cotransport in rabbit renal cortical basolateral membrane vesicles. Journal Of Clinical Investigation 1986, 78: 1472-1478. PMID: 3782468, PMCID: PMC423898, DOI: 10.1172/jci112738.Peer-Reviewed Original ResearchConceptsMembrane vesiclesBasolateral membrane vesiclesSodium uptakePotassium diffusion potentialBicarbonate cotransporterSaturable stimulationSodium/bicarbonate cotransporterVesiclesRabbit renal cortexProteinDisulfonic stilbeneBicarbonate transportPresence of bicarbonateUptakeKinetic analysisRenal cortexCell pH in the rat proximal convoluted tubule. Regulation by luminal and peritubular pH and sodium concentration.
Alpern RJ, Chambers M. Cell pH in the rat proximal convoluted tubule. Regulation by luminal and peritubular pH and sodium concentration. Journal Of Clinical Investigation 1986, 78: 502-510. PMID: 3016029, PMCID: PMC423587, DOI: 10.1172/jci112602.Peer-Reviewed Original Research
1983
Flow dependence of proximal tubular bicarbonate absorption
Alpern RJ, Cogan MG, Rector FC. Flow dependence of proximal tubular bicarbonate absorption. American Journal Of Physiology 1983, 245: f478-f484. PMID: 6414312, DOI: 10.1152/ajprenal.1983.245.4.f478.Peer-Reviewed Original ResearchMeSH KeywordsAbsorptionAnimalsBicarbonatesBiological TransportCarbon DioxideKidney Tubules, ProximalKineticsMaleModels, BiologicalPerfusionRatsRats, Inbred StrainsConceptsLuminal bicarbonate concentrationBicarbonate absorptionFlow-dependent increaseFlow-dependent decreaseProximal convoluted tubulesBicarbonate concentrationFlow-induced increaseFlow-dependent changesConvoluted tubulesX minPmol xStimulationProton secretionTubulesPerfusion rateMaximal rateObserved stimulationMin
1982
Effect of luminal bicarbonate concentration on proximal acidification in the rat
Alpern RJ, Cogan MG, Rector FC. Effect of luminal bicarbonate concentration on proximal acidification in the rat. American Journal Of Physiology 1982, 243: f53-f59. PMID: 7091367, DOI: 10.1152/ajprenal.1982.243.1.f53.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicarbonatesBiological TransportHydrogen-Ion ConcentrationKidney Tubules, ProximalKineticsMaleRatsRats, Inbred StrainsConceptsLuminal bicarbonate concentrationBicarbonate absorptionBicarbonate concentrationNet bicarbonate absorptionProximal convoluted tubulesProximal tubular acidificationProton secretionBicarbonate leakProximal acidificationTubular acidificationConvoluted tubulesBicarbonate permeabilitySecretionRats