2004
A consensus sequence in the endothelin-B receptor second intracellular loop is required for NHE3 activation by endothelin-1
Laghmani K, Sakamoto A, Yanagisawa M, Preisig PA, Alpern RJ. A consensus sequence in the endothelin-B receptor second intracellular loop is required for NHE3 activation by endothelin-1. American Journal Of Physiology. Renal Physiology 2004, 288: f732-f739. PMID: 15598844, DOI: 10.1152/ajprenal.00300.2004.Peer-Reviewed Original ResearchConceptsEndothelin-1Second intracellular loopET-1 stimulationNHE3 activityIntracellular loopEndothelin-A receptorAdenylyl cyclase inhibitionET-1 regulationProximal tubule apical membraneET receptorsCyclase inhibitionOKP cellsNHE3 activationOpossum kidneyExchanger 3ReceptorsStimulationProtein tyrosine phosphorylationReceptor chimerasPresent studyCellsAdditional mechanismTyrosine phosphorylationApical membraneSimilar pattern
1999
Incubation of OKP cells in low-K+ media increases NHE3 activity after early decrease in intracellular pH
Amemiya M, Tabei K, Kusano E, Asano Y, Alpern R. Incubation of OKP cells in low-K+ media increases NHE3 activity after early decrease in intracellular pH. American Journal Of Physiology 1999, 276: c711-c716. PMID: 10069999, DOI: 10.1152/ajpcell.1999.276.3.c711.Peer-Reviewed Original ResearchConceptsOKP cellsEarly decreaseNHE3 activityNHE3 protein abundanceTyrosine kinase inhibitorsProximal tubule apical membraneChronic hypokalemiaTyrosine kinase pathwayIntracellular acidosisNHE3 mRNAKinase inhibitorsControl levelsNHE3Herbimycin AKinase pathwayControl mediumPresent studySodium propionateIntracellularCellsAntiporter activityApical membraneHypokalemiaIncubationProtein abundance
1997
Adaptation to low-K+ media increases H(+)-K(+)-ATPase but not H(+)-ATPase-mediated pHi recovery in OMCD1 cells
Guntupalli J, Onuigbo M, Wall S, Alpern RJ, DuBose TD. Adaptation to low-K+ media increases H(+)-K(+)-ATPase but not H(+)-ATPase-mediated pHi recovery in OMCD1 cells. American Journal Of Physiology 1997, 273: c558-c571. PMID: 9277353, DOI: 10.1152/ajpcell.1997.273.2.c558.Peer-Reviewed Original Research
1996
Glucocorticoids regulate NHE-3 transcription in OKP cells
Baum M, Amemiya M, Dwarakanath V, Alpern RJ, Moe OW. Glucocorticoids regulate NHE-3 transcription in OKP cells. American Journal Of Physiology 1996, 270: f164-f169. PMID: 8769835, DOI: 10.1152/ajprenal.1996.270.1.f164.Peer-Reviewed Original ResearchConceptsNHE-3 mRNA abundanceGlucocorticoid-mediated increaseOKP cellsDexamethasone-treated cellsNHE-3 mRNAMRNA abundanceTwo- to threefold increaseAntiporter activitySynthetic glucocorticoidProximal tubulesApical proton secretionM dexamethasoneDexamethasoneNHE-3GlucocorticoidsTime courseProton secretionThreefold increasePresent studyCellsGene transcriptionMRNASecretionActivity
1994
Acid activation of immediate early genes in renal epithelial cells.
Yamaji Y, Moe OW, Miller RT, Alpern RJ. Acid activation of immediate early genes in renal epithelial cells. Journal Of Clinical Investigation 1994, 94: 1297-1303. PMID: 8083371, PMCID: PMC295215, DOI: 10.1172/jci117448.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAcidosisAnimalsCell LineCell NucleusDNA-Binding ProteinsEarly Growth Response Protein 1EpitheliumGene Expression RegulationGenes, Immediate-EarlyHydrogen-Ion ConcentrationImmediate-Early ProteinsIonomycinKidney Tubules, ProximalMiceProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junRNA, MessengerTetradecanoylphorbol AcetateTranscription FactorsTranscriptional ActivationConceptsTyrosine kinase pathwayGene activationKinase pathwayMouse proximal tubule cell lineC-JunRate of transcriptionProtein kinase CImmediate early genesRenal epithelial cellsMRNA speciesPretreatment of cellsGene expressionKinase CEarly genesGenesMRNA abundanceProximal tubule cell lineCell linesTubule cell lineRenal tubule cellsEpithelial cellsC-fosGrowth factorCellsMRNA
1993
Glucocorticoids stimulate Na+/H+ antiporter in OKP cells
Baum M, Cano A, Alpern RJ. Glucocorticoids stimulate Na+/H+ antiporter in OKP cells. American Journal Of Physiology 1993, 264: f1027-f1031. PMID: 8391752, DOI: 10.1152/ajprenal.1993.264.6.f1027.Peer-Reviewed Original ResearchConceptsOKP cellsProximal tubule acidificationEffect of dexamethasoneAntiporter activityTime-dependent stimulationM aldosteroneHemodynamic changesTubule acidificationSystemic administrationDependent pH recoveryDexamethasoneM dexamethasoneAcid loadStimulatory effectGlucocorticoidsPH recoveryStimulationPH-sensitive dyePresent studyMaximal velocityCellsDirect effectAldosteronePrevious studiesActivity
1991
Chronic adaptations in proximal tubular H/HCO3 transporters.
Alpern RJ, Horie S, Moe O, Tejedor A, Miller RT, Preisig PA. Chronic adaptations in proximal tubular H/HCO3 transporters. Kidney International Supplement 1991, 33: s29-32. PMID: 1653870.Peer-Reviewed Original ResearchConceptsCultured proximal tubule cellsChronic hyperfiltrationNa/H antiporterProximal tubule cellsChronic acidosisTubule cellsNa/H antiporter activityH antiporterK deficiencyEffect of acidosisExtracellular fluid pHChronic adaptationsAcidosisHyperfiltrationCotransporter activityAbundance of mRNAParallel increaseHCO3- transportersAcid preincubationDeficiencyFluid pHCellsAntiporter activityProtein synthesisExtracellular signals
1990
Preincubation in acid medium increases Na/H antiporter activity in cultured renal proximal tubule cells.
Horie S, Moe O, Tejedor A, Alpern RJ. Preincubation in acid medium increases Na/H antiporter activity in cultured renal proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 4742-4745. PMID: 2162055, PMCID: PMC54193, DOI: 10.1073/pnas.87.12.4742.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityProximal tubule cellsTubule cellsCultured renal proximal tubule cellsCultured proximal tubule cellsRenal proximal tubule cellsPreincubation of fibroblastsExtracellular fluidAntiporter activityChronic acidosisAcidotic environmentCO2/HCO3Small inhibitionPreincubationPreincubation mediumAddition of cycloheximideCell acidificationAcid preincubationPresent studyProtein synthesisCellsAcidosisActivityEpithelium
1988
Chronic metabolic acidosis causes an adaptation in the apical membrane Na/H antiporter and basolateral membrane Na(HCO3)3 symporter in the rat proximal convoluted tubule.
Preisig PA, Alpern RJ. Chronic metabolic acidosis causes an adaptation in the apical membrane Na/H antiporter and basolateral membrane Na(HCO3)3 symporter in the rat proximal convoluted tubule. Journal Of Clinical Investigation 1988, 82: 1445-1453. PMID: 2844858, PMCID: PMC442703, DOI: 10.1172/jci113750.Peer-Reviewed Original ResearchConceptsChronic metabolic acidosisNa/H antiporterMetabolic acidosisAcidotic animalsH antiporterVivo microperfused rat proximal tubuleDpHi/dtRat proximal tubuleBasolateral membraneTransporter activityDietary acidPeritubular capillariesProximal tubulesNa/HPH-sensitive fluorescent dyeAcidosisTubulesSubsequent studiesAnimalsCarboxy-fluoresceinAbsence of contactCells
1987
Basolateral membrane Na/base cotransport is dependent on CO2/HCO3 in the proximal convoluted tubule.
Krapf R, Alpern RJ, Rector FC, Berry CA. Basolateral membrane Na/base cotransport is dependent on CO2/HCO3 in the proximal convoluted tubule. The Journal Of General Physiology 1987, 90: 833-853. PMID: 2831294, PMCID: PMC2228880, DOI: 10.1085/jgp.90.6.833.Peer-Reviewed Original ResearchBasolateral membrane Cl/HCO3 exchange in the rat proximal convoluted tubule. Na-dependent and -independent modes.
Alpern RJ, Chambers M. Basolateral membrane Cl/HCO3 exchange in the rat proximal convoluted tubule. Na-dependent and -independent modes. The Journal Of General Physiology 1987, 89: 581-598. PMID: 2953859, PMCID: PMC2215917, DOI: 10.1085/jgp.89.4.581.Peer-Reviewed Original ResearchConceptsCl/HCO3 exchangeNa-free solutionHCO3- exchangePeritubular NaCell pHHCO3- transport mechanismsBasolateral membraneProximal tubulesCoupling of NaMammalian proximal tubuleMeq/Similar increaseAmbient ClExtracellular fluidTubulesNa dependenceCotransporterNa concentrationCl concentrationsCellsSimilar amounts
1985
Mechanism of basolateral membrane H+/OH-/HCO-3 transport in the rat proximal convoluted tubule. A sodium-coupled electrogenic process.
Alpern RJ. Mechanism of basolateral membrane H+/OH-/HCO-3 transport in the rat proximal convoluted tubule. A sodium-coupled electrogenic process. The Journal Of General Physiology 1985, 86: 613-636. PMID: 2999293, PMCID: PMC2228817, DOI: 10.1085/jgp.86.5.613.Peer-Reviewed Original ResearchConceptsUnits/minPeritubular pHHigh extracellular potassium concentrationCell pHSodium concentrationPotassium concentrationExtracellular potassium concentrationBasolateral membranePeritubular potassium concentrationCell depolarizationMeq/Sodium removalCell acidificationTubulesPeritubular fluidTotal absenceSitCellsRatsMinSodium