2001
Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa
Eickhoff R, Wilhelm B, Renneberg H, Wennemuth G, Bacher M, Linder D, Bucala R, Seitz J, Meinhardt A. Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa. Molecular Medicine 2001, 7: 27-35. PMID: 11474125, PMCID: PMC1949991, DOI: 10.1007/bf03401836.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEpididymisImmunohistochemistryMacrophage Migration-Inhibitory FactorsMaleMicroscopy, ImmunoelectronRatsRNA, MessengerSpermatozoaConceptsOuter dense fibersSequence analysisDense fibersSecretion modeCDNA fragmentsN-terminal signal sequenceEndoplasmic reticulumMacrophage migration inhibitory factorEpithelial cellsCytokine macrophage migration inhibitory factorN-terminal amino acid sequence analysisAmino acid sequence analysisApical cell surfaceAcid sequence analysisEpididymal epithelial cellsRat epididymisSignal sequenceCDNA sequenceWestern blot experimentsPlasma membraneMigration inhibitory factorSecretory proteinsCytoskeletal elementsExpression patternsN-terminus
1998
Increased Levels of Advanced Glycation Endproducts in the Lenses and Blood Vessels of Cigarette Smokers
Nicholl I, Stitt A, Moore J, Ritchie A, Archer D, Bucala R. Increased Levels of Advanced Glycation Endproducts in the Lenses and Blood Vessels of Cigarette Smokers. Molecular Medicine 1998, 4: 594-601. PMID: 9848076, PMCID: PMC2230315, DOI: 10.1007/bf03401759.Peer-Reviewed Original ResearchMeSH KeywordsAgedCataractCataract ExtractionCoronary VesselsFemaleGlycation End Products, AdvancedHumansLens, CrystallineMaleMicroscopy, ImmunoelectronSmokingConceptsNondiabetic smokersTissues of smokersCoronary arteryGlycation endproductsSingle greatest preventable causeGreatest preventable causeEnd-organ complicationsAnti-AGE antibodyConsequence of diabetesAdvanced glycation endproductsRenal insufficiencyCigarette smokersPreventable causeSmokersCigarette smokeHigh levelsCausal associationNonsmokersDiabetesMainstream cigarette smokeBlood vesselsAgeExtracellular matrix proteinsArteryLens fiber cells
1995
Localization of Macrophage Migration Inhibitory Factor (MIF) to Secretory Granules within the Corticotrophic and Thyrotrophic Cells of the Pituitary Gland
Nishino T, Bernhagen J, Shiiki H, Calandra T, Dohi K, Bucala R. Localization of Macrophage Migration Inhibitory Factor (MIF) to Secretory Granules within the Corticotrophic and Thyrotrophic Cells of the Pituitary Gland. Molecular Medicine 1995, 1: 781-788. PMID: 8612200, PMCID: PMC2230018, DOI: 10.1007/bf03401892.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorRelease of MIFCorticotropin-releasing hormoneMigration inhibitory factorAnterior pituitary glandPituitary glandThyrotrophic cellsBackgroundMacrophage migration inhibitory factorInhibitory factorCorticotrophic cellsAnterior pituitary hormonesActivated T lymphocytesHormone-specific antibodiesSubsets of granulesGlucocorticoid suppressionSeptic shockLPS injectionCytokine productionEndotoxin administrationACTH releaseT lymphocytesConclusionsThese dataPituitary hormonesPituitary contentHost response