2000
Immunological Evidence that Non-carboxymethyllysine Advanced Glycation End-products Are Produced from Short Chain Sugars and Dicarbonyl Compounds in vivo
Takeuchi M, Makita Z, Bucala R, Suzuki T, Koike T, Kameda Y. Immunological Evidence that Non-carboxymethyllysine Advanced Glycation End-products Are Produced from Short Chain Sugars and Dicarbonyl Compounds in vivo. Molecular Medicine 2000, 6: 114-125. PMID: 10859028, PMCID: PMC1949938, DOI: 10.1007/bf03401779.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesCattleChromatography, AffinityCross ReactionsDiabetes Mellitus, Type 2Enzyme-Linked Immunosorbent AssayGlycation End Products, AdvancedGlyceraldehydeGlyoxalHumansImmunoblottingKidney Failure, ChronicLysineMaillard ReactionOxidation-ReductionPyruvaldehydeRabbitsRenal DialysisConceptsAGE antibodyCML-AGEDiabetic patientsAGE-bovine serum albuminShort Chain SugarsAdvanced glycation end productsAnti-AGE antibodyGlycation end productsAutoxidation of sugarsImmunization of rabbitsCML-BSADiabetic serumSerum albuminAdvanced glycationAGE contentSugar autoxidationRabbit serum albuminAGE modificationAntibodiesImmunological evidenceApparent molecular weightPatientsAge 3AgeImmunoblot analysis
1999
Detoxification of Methylglyoxal by the Nucleophilic Bidentate, Phenylacylthiazolium Bromide
Ferguson G, VanPatten S, Bucala R, Al-Abed Y. Detoxification of Methylglyoxal by the Nucleophilic Bidentate, Phenylacylthiazolium Bromide. Chemical Research In Toxicology 1999, 12: 617-622. PMID: 10409401, DOI: 10.1021/tx990007y.Peer-Reviewed Original ResearchConceptsEscherichia coli double mutantRapid cell deathE. coli cellsDetoxification of methylglyoxalExponential-phase cellsDouble mutantSensitive bacterial assayColi cellsCell deathPhase cellsE. coliLong-term complicationsPotassium channelsReactive dicarbonylsBacterial assaysDetoxificationEndogenous nucleophilesCellsMethylglyoxalTissue damage