1999
Cloning and Characterization of the Murine Histone Deacetylase (HDAC3)
Mahlknecht U, Hoelzer D, Bucala R, Verdin E. Cloning and Characterization of the Murine Histone Deacetylase (HDAC3). Biochemical And Biophysical Research Communications 1999, 263: 482-490. PMID: 10491319, DOI: 10.1006/bbrc.1999.1389.Peer-Reviewed Original ResearchConceptsHistone deacetylasesCore histone proteinsHuman chromosome 5q31Potential tumor suppressor geneHistone acetylation modifiersMalignant myeloid diseasesTumor suppressor geneHistone proteinsTranscriptional complexHistone acetylationDevelopment of cancerHistone deacetylaseSuppressor geneHDAC3Chromosome 5q31Cellular proliferationEnzymatic activityAcetylationMyeloid diseasesImportant insightsOrthologsHistonesCloningDeacetylasesGenes
1997
Biochemical and Mutational Investigations of the Enzymatic Activity of Macrophage Migration Inhibitory Factor †
Bendrat K, Al-Abed Y, Callaway D, Peng T, Calandra T, Metz C, Bucala R. Biochemical and Mutational Investigations of the Enzymatic Activity of Macrophage Migration Inhibitory Factor †. Biochemistry 1997, 36: 15356-15362. PMID: 9398265, DOI: 10.1021/bi971153a.Peer-Reviewed Original ResearchConceptsEnzymatic activityL-dopachrome methyl esterN-terminal proline residueSite-directed mutagenesisAmino acid residuesDopachrome tautomerase activityNative solution conditionsMutant proteinsProtein domainsStable trimer formationCarboxy terminusBasic residuesN-terminusProline residuesSDS-PAGE analysisBacterial enzymesHomotrimeric structureAcid residuesCatalytic functionEnzymatic mechanismMutational investigationTrimer formationLow protein concentrationsBiochemical analysisInternal histidine