1995
Formation of Immunochemical Advanced Glycosylation End Products Precedes and Correlates With Early Manifestations of Renal and Retinal Disease in Diabetes
Beisswenger P, Makita Z, Curphey T, Moore L, Jean S, Brinck-Johnsen T, Bucala R, Vlassara H. Formation of Immunochemical Advanced Glycosylation End Products Precedes and Correlates With Early Manifestations of Renal and Retinal Disease in Diabetes. Diabetes 1995, 44: 824-829. PMID: 7789650, DOI: 10.2337/diab.44.7.824.Peer-Reviewed Original ResearchConceptsAdvanced glycosylation end productsEnzyme-linked immunosorbent assayAlbumin excretionDuration of diabetesElevated levelsNormal renal statusTissue advanced glycosylation end productsDiabetic vascular complicationsTissue AGE levelsGlycosylation end productsEvident retinopathyMicroalbuminuric phaseOvert microangiopathySevere retinopathyVascular complicationsDiabetic nephropathyRenal statusUrinary albuminProliferative retinopathyEarly retinopathyHigher age levelsSignificant elevationRetinopathyRetinal diseasesEarly manifestation
1985
Increased Levels of 16α-Hydroxyestrone-Modified Proteins in Pregnancy and in Systemic Lupus Erythematosus*
BUCALA R, LAHITA R, FISHMAN J, CERAMI A. Increased Levels of 16α-Hydroxyestrone-Modified Proteins in Pregnancy and in Systemic Lupus Erythematosus*. The Journal Of Clinical Endocrinology & Metabolism 1985, 60: 841-847. PMID: 3920233, DOI: 10.1210/jcem-60-5-841.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusLupus erythematosusPregnant womenHigher mean levelsNormal womenAlpha-hydroxyestroneFemale hormonesNormal subjectsErythematosusNormal levelsElevated levelsMean levelsWomenLatter groupRed cellsLymphocyte proteinSimilar elevationsPregnancyOHEBasement membrane proteinsUseful indicatorLevelsPatientsModification of proteinsLymphocytes
1984
The reaction of 16α‐hydroxyestrone with erythrocytes in vitro and in vivo
BUCALA R, FISHMAN J, CERAMI A. The reaction of 16α‐hydroxyestrone with erythrocytes in vitro and in vivo. The FEBS Journal 1984, 140: 593-598. PMID: 6723653, DOI: 10.1111/j.1432-1033.1984.tb08143.x.Peer-Reviewed Original ResearchConceptsMembrane proteinsCellular proteinsCell lifeRed cell lifeLysine residuesProteinCovalent adductsHydrophobic chromatographyAcid-precipitable radioactivityRate of incorporationStable covalent adductCellsReverse-phase high-pressure liquid chromatographyElevated levelsResiduesAdduct formationRed cellsElectrophoresisVitro
1983
Characterization of antisera to the addition product formed by the nonenzymatic reaction of 16α-hydroxyestrone with albumin
Bucala R, Cerami A. Characterization of antisera to the addition product formed by the nonenzymatic reaction of 16α-hydroxyestrone with albumin. Molecular Immunology 1983, 20: 1289-1292. PMID: 6656775, DOI: 10.1016/0161-5890(83)90159-1.Peer-Reviewed Original ResearchConceptsSystemic lupus erythematosusLupus erythematosusAutoimmune diseasesAlpha-hydroxyestroneHigh-titre antiseraLack of specificityCross-reactivity analysisPhenolic A-ringElevated levelsCharacterization of antiseraLow molar substitutionOHEAntiserumImmunochemical analysisHigh specificityAlbuminHigh affinity