2001
Identification of a chloride-formate exchanger expressed on the brush border membrane of renal proximal tubule cells
Knauf F, Yang C, Thomson R, Mentone S, Giebisch G, Aronson P. Identification of a chloride-formate exchanger expressed on the brush border membrane of renal proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 9425-9430. PMID: 11459928, PMCID: PMC55437, DOI: 10.1073/pnas.141241098.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmino Acid SequenceAnimalsAntiportersBase SequenceCloning, MolecularCOS CellsDNA PrimersDNA, ComplementaryFemaleKidney Tubules, ProximalMiceMice, Inbred C57BLMicroscopy, FluorescenceMicrovilliMolecular Sequence DataSodium-Phosphate Cotransporter Proteins, Type IIaXenopus laevis
1999
Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity
Whyte D, Li C, Thomson R, Nix S, Zanjani R, Karp S, Aronson P, Igarashi P. Ksp-cadherin gene promoter. I. Characterization and renal epithelial cell-specific activity. American Journal Of Physiology 1999, 277: f587-f598. PMID: 10516284, DOI: 10.1152/ajprenal.1999.277.4.f587.Peer-Reviewed Original ResearchConceptsHepatocyte nuclear factor 3Activator protein-2Renal epithelial cellsCAAT boxNuclear proteinsFlanking regionsPromoter activityTissue-specific gene expressionEpithelial cellsElectrophoretic mobility shift assaysKsp-cadherin gene promoterCCAAT/enhancer-binding proteinTranscription initiation siteKidney-specific memberMobility shift assaysConsensus promoter elementsEnhancer-binding proteinCell-specific activityPotential binding sitesLambda bacteriophage clonesLuciferase reporter plasmidHelix proteinsKidney epithelial cellsGATA factorsGC box
1998
cDNA Cloning and Chromosomal Localization of the Human and Mouse Isoforms of Ksp-Cadherin
Thomson RB, Ward DC, Quaggin SE, Igarashi P, Muckler ZE, Aronson PS. cDNA Cloning and Chromosomal Localization of the Human and Mouse Isoforms of Ksp-Cadherin. Genomics 1998, 51: 445-451. PMID: 9721215, DOI: 10.1006/geno.1998.5402.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCadherinsCell Adhesion MoleculesChromosome MappingChromosomes, Human, Pair 16Cloning, MolecularHumansIn Situ Hybridization, FluorescenceKidneyMiceMice, Inbred StrainsMolecular Sequence DataMultigene FamilyRNA, MessengerSequence Analysis, DNASequence Homology, Amino AcidConceptsMouse isoformsKsp-cadherinPutative N-glycosylation sitesInferred amino acid sequenceSingle ancestral geneCharacteristic protein domainsCadherin family membersKidney-specific memberN-glycosylation sitesAmino acid sequenceMammalian genomesAncestral geneGene duplicationSyntenic regionsChromosomal localizationComplete cDNAGene mapsProtein domainsSequence motifsGene sequencesCDNA cloningCell adhesion moleculeAcid sequenceNorthern analysisChromosome 8
1992
cDNA cloning and localization of a band 3-related protein from ileum
Chow A, Dobbins JW, Aronson PS, Igarashi P. cDNA cloning and localization of a band 3-related protein from ileum. American Journal Of Physiology 1992, 263: g345-g352. PMID: 1415547, DOI: 10.1152/ajpgi.1992.263.3.g345.Peer-Reviewed Original ResearchConceptsRabbit ileal enterocytesDeduced amino acid sequenceBand 3Anion exchanger familyAmino acid sequenceAE gene familyBrush border membraneGene familyNonerythroid tissuesHCO3- exchangerErythroid band 3CDNA cloningPlasma membraneAcid sequenceExchanger familyRelated proteinsMembrane vesiclesIleal enterocytesMolecular identityCytoplasmic fragmentsProteinBasolateral membrane vesiclesBasolateral membraneMembraneEnterocytes
1991
cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells
Reilly RF, Hildebrandt F, Biemesderfer D, Sardet C, Pouyssegur J, Aronson PS, Slayman CW, Igarashi P. cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells. American Journal Of Physiology 1991, 261: f1088-f1094. PMID: 1661081, DOI: 10.1152/ajprenal.1991.261.6.f1088.Peer-Reviewed Original ResearchConceptsLLC-PK1 cellsFusion proteinAmino-terminal hydrophobic regionLLC-PK1 renal epithelial cellsHydrophobic regionAmino acid sequenceConfluent LLC-PK1 cellsRelative molecular massRenal epithelial cellsSignificant homologyHuman proteinsCDNA cloningAcid sequenceNucleotide identityHuman sequenceLibrary screeningCDNAMolecular massPolymerase chain reactionAmino acidsApical membraneProteinConfocal microscopyHydrophilic domainsLLC-PK1Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript
Hildebrandt F, Pizzonia J, Reilly R, Rebouças N, Sardet C, Pouysségur J, Slayman C, Aronson P, Igarashi P. Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript. Biochimica Et Biophysica Acta 1991, 1129: 105-108. PMID: 1661611, DOI: 10.1016/0167-4781(91)90221-7.Peer-Reviewed Original ResearchMolecular biology of renal Na(+)-H+ exchangers.
Igarashi P, Reilly RF, Hildebrandt F, Biemesderfer D, Rebouças NA, Slayman CW, Aronson PS. Molecular biology of renal Na(+)-H+ exchangers. Kidney International Supplement 1991, 33: s84-9. PMID: 1653876.Commentaries, Editorials and LettersMeSH KeywordsAcidosisAnimalsCarrier ProteinsCell MembraneCloning, MolecularDNAHumansKidneyRabbitsSodium-Hydrogen ExchangersSwineTissue DistributionConceptsKb cDNALLC-PK1 cellsSteady-state transcript levelsPlasma membrane proteinsMembrane-associated domainSequence of cDNACDNA library screeningAmino acid sequenceExchanger gene expressionNorthern blot analysisEvolutionary conservationRenal epithelial cellsCytoplasmic domainMembrane proteinsRegulation of intracellularAcid sequenceTranscript levelsUntranslated regionGene expressionSimilar transcriptsCDNALibrary screeningMolecular biologyPolymerase chain reactionBlot analysis