2002
Role of c-SRC and ERK in acid-induced activation of NHE3
Tsuganezawa H, Sato S, Yamaji Y, Preisig PA, Moe OW, Alpern RJ. Role of c-SRC and ERK in acid-induced activation of NHE3. Kidney International 2002, 62: 41-50. PMID: 12081562, DOI: 10.1046/j.1523-1755.2002.00418.x.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmonium ChlorideAnimalsCells, CulturedCSK Tyrosine-Protein KinaseEnzyme ActivationJNK Mitogen-Activated Protein KinasesMaleMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleySodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSrc-Family KinasesConceptsOKP cellsAcid-induced activationNHE3 activityERK/c-fosC-fos expressionC-Fos activationCytoplasmic pH (pHi) recoveryC-SrcRenal proximal tubulesAcid incubationActivation of NHE3Renal epithelial cellsCultured renal epithelial cellsProximal tubulesChronic acidosisInhibition of MEKNHE3 activationInhibited activationNormal levelsAcid loadImmune complex kinase assayERK kinaseAcidosisPH recoveryEpithelial cells
2001
Endothelin-1/endothelin-B receptor–mediated increases in NHE3 activity in chronic metabolic acidosis
Laghmani K, Preisig P, Moe O, Yanagisawa M, Alpern R. Endothelin-1/endothelin-B receptor–mediated increases in NHE3 activity in chronic metabolic acidosis. Journal Of Clinical Investigation 2001, 107: 1563-1569. PMID: 11413164, PMCID: PMC200190, DOI: 10.1172/jci11234.Peer-Reviewed Original ResearchMeSH KeywordsAcidosisAmmoniaAnimalsBicarbonatesChronic DiseaseCitric AcidCulture TechniquesEndothelin-1EndothelinsKidney Tubules, ProximalMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicProtein PrecursorsReceptor, Endothelin BReceptors, EndothelinRNA, MessengerSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsTg/tgAcid ingestionTitratable acid excretionMetabolic acidosisNHE3 activityAcid excretionReceptor-deficient miceEndothelin B receptorET-1 expressionChronic metabolic acidosisProximal tubule suspensionsAmmonium excretionM endothelinNa/H antiporterC57BL/6 miceApical membrane NHE3Renal epithelial cellsCultured renal epithelial cellsEndothelin-1/Tubule suspensionsMRNA expressionControl dietMiceExcretionEpithelial cells
1999
A cell cycle-dependent mechanism of renal tubule epithelial cell hypertrophy
Preisig P. A cell cycle-dependent mechanism of renal tubule epithelial cell hypertrophy. Kidney International 1999, 56: 1193-1198. PMID: 10610409, DOI: 10.1046/j.1523-1755.1999.00708.x.Peer-Reviewed Original ResearchTGF-β1-mediated hypertrophy involves inhibiting pRB phosphorylation by blocking activation of cyclin E kinase
Liu B, Preisig P. TGF-β1-mediated hypertrophy involves inhibiting pRB phosphorylation by blocking activation of cyclin E kinase. American Journal Of Physiology 1999, 277: f186-f194. PMID: 10444572, DOI: 10.1152/ajprenal.1999.277.2.f186.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCDC2-CDC28 KinasesCdc25 PhosphatasesCell Cycle ProteinsCell LineCyclin ECyclin-Dependent Kinase 2Cyclin-Dependent Kinase 4Cyclin-Dependent Kinase Inhibitor p27Cyclin-Dependent Kinase Inhibitor p57Cyclin-Dependent KinasesEnzyme ActivationEpidermal Growth FactorHypertrophyKidneyMicrotubule-Associated ProteinsNuclear ProteinsPhosphorylationProtein Serine-Threonine KinasesProtein Tyrosine PhosphatasesProto-Oncogene ProteinsRatsRetinoblastoma ProteinTransforming Growth Factor betaTumor Suppressor ProteinsConceptsCdk2/cyclin E complexesCyclin E complexCyclin E kinaseGrowth responseE complexCell cycle progressionRenal epithelial cellsProtein abundanceRetinoblastoma proteinKinase activityCycle progressionHypophosphorylated statePRB phosphorylationHypertrophic growth responseKinaseCyclin EAbundanceEpithelial cellsActive stateDevelopment of hypertrophyActivationCell entranceComplexesArrestTGF-β1
1997
Media acidification inhibits TGF beta-mediated growth suppression in cultured rabbit proximal tubule cells.
Tovbin D, Franch HA, Alpern RJ, Preisig PA. Media acidification inhibits TGF beta-mediated growth suppression in cultured rabbit proximal tubule cells. Proceedings Of The Association Of American Physicians 1997, 109: 572-9. PMID: 9394419.Peer-Reviewed Original ResearchConceptsDirect antiproliferative effectRabbit proximal tubule cellsProximal tubule cellsAntiproliferative effectsGrowth-suppressive effectsMetabolic acidosisCultured rabbit proximal tubule cellsTubule cellsChronic metabolic acidosisInhibits cell cycle progressionRenal functionRenal growthMitogen-stimulated cellsRenal epithelial cellsProgressive lossHyperplasiaHypertrophyCell cycle progressionEpithelial cellsPrimary culturesPRB phosphorylationAcidosisEGF-induced phosphorylationGrowth suppressionCycle progression
1996
NH4Cl-induced hypertrophy is mediated by weak base effects and is independent of cell cycle processes
Franch H, Preisig P. NH4Cl-induced hypertrophy is mediated by weak base effects and is independent of cell cycle processes. American Journal Of Physiology 1996, 270: c932-c938. PMID: 8638677, DOI: 10.1152/ajpcell.1996.270.3.c932.Peer-Reviewed Original ResearchConceptsC-fos mRNA abundanceHuman papilloma virus 16NRK-52E cellsWeak base propertiesCell cycle processRenal hypertrophyClinical conditionsVirus 16Renal epithelial cellsCultured renal epithelial cellsCell cycleHypertrophyE7 genesAdditional studiesEpithelial cellsVesicular alkalinizationMRNA abundancePresent studyInactivation of pRbCellsProtein synthesisVacuolar proton pumpProtein abundanceVesicular compartments
1995
Renal epithelial cell hyperplasia and hypertrophy.
Preisig P, Franch H. Renal epithelial cell hyperplasia and hypertrophy. Seminars In Nephrology 1995, 15: 327-40. PMID: 7569412.Peer-Reviewed Original ResearchConceptsCell cycleNumber of proteinsCell hypertrophyRenal epithelial cellsDNA replicationProtein degradationCell protein contentCell growthGrowth responseCascade of eventsEpithelial cell hyperplasiaMitogenic stimuliIndependent mechanismsForm of hypertrophyEpithelial cellsSequential eventsLysosomal enzymesCurrent understandingPathological processesCell hyperplasiaProtein contentCellsKey roleEpithelial cell hypertrophyRate of growth
1991
Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts.
Moe OW, Miller RT, Horie S, Cano A, Preisig PA, Alpern RJ. Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts. Journal Of Clinical Investigation 1991, 88: 1703-1708. PMID: 1658050, PMCID: PMC295708, DOI: 10.1172/jci115487.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityRenal tubule cellsNa/H antiporterMCT cellsRenal epithelial cellsTubule cellsCell linesCultured renal tubule cellsEpithelial cellsMRNA abundanceOpossum kidney cell lineChronic metabolic acidosisAcid preincubationTubule cell lineRat renal cortexH antiporterAntiporter activityUnderlying molecular mechanismsDifferential regulationMetabolic acidosisRenal cortexAcidotic environmentAcid feedingAdaptive increaseKidney cell line