2023
Homeostatic, Non-Canonical Role of Macrophage Elastase in Vascular Integrity
Salarian M, Ghim M, Toczek J, Han J, Weiss D, Spronck B, Ramachandra A, Jung J, Kukreja G, Zhang J, Lakheram D, Kim S, Humphrey J, Sadeghi M. Homeostatic, Non-Canonical Role of Macrophage Elastase in Vascular Integrity. Circulation Research 2023, 132: 432-448. PMID: 36691905, PMCID: PMC9930896, DOI: 10.1161/circresaha.122.322096.Peer-Reviewed Original ResearchConceptsMMP-12 deficiencyAdverse aortic remodelingAbdominal aortic aneurysmAng IIAortic remodelingAortic aneurysmMMP-12Complement component 3 levelsNeutrophil extracellular traps markersAbdominal aortic aneurysm ruptureAortic aneurysm ruptureElastic lamina degradationPlasma complement componentsAortic ruptureC3 levelsComplement depositionPlasma C5aMore neutrophilsVascular remodelingAneurysm ruptureNeutrophil elastaseAortic integrityMatrix metalloproteinaseComplement inhibitorsNETosis pathway
2021
Computed tomography imaging of macrophage phagocytic activity in abdominal aortic aneurysm
Toczek J, Boodagh P, Sanzida N, Ghim M, Salarian M, Gona K, Kukreja G, Rajendran S, Wei L, Han J, Zhang J, Jung JJ, Graham M, Liu X, Sadeghi MM. Computed tomography imaging of macrophage phagocytic activity in abdominal aortic aneurysm. Theranostics 2021, 11: 5876-5888. PMID: 33897887, PMCID: PMC8058712, DOI: 10.7150/thno.55106.Peer-Reviewed Original ResearchConceptsAbdominal aortic aneurysmExiTron nano 12000AAA outcomePhagocytic activityII infusionAng IIAortic aneurysmAortic wall enhancementAng II infusionCT enhancementAngiotensin II infusionRole of inflammationFeasibility of CTMacrophage phagocytic activityNon-invasive toolAAA inductionCD68 expressionModulatory interventionsMacrophage cell lineInflammatory signalsPatient managementVascular pathologyOutcome studiesAdventitial macrophagesComputed tomography
2014
Imaging Vessel Wall Biology to Predict Outcome in Abdominal Aortic Aneurysm
Golestani R, Razavian M, Nie L, Zhang J, Jung JJ, Ye Y, de Roo M, Hilgerink K, Liu C, Robinson SP, Sadeghi MM. Imaging Vessel Wall Biology to Predict Outcome in Abdominal Aortic Aneurysm. Circulation Cardiovascular Imaging 2014, 8: &na;. PMID: 25550400, PMCID: PMC4284949, DOI: 10.1161/circimaging.114.002471.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticAorta, AbdominalAortic Aneurysm, AbdominalAortic RuptureAortographyBiomarkersDisease Models, AnimalDisease ProgressionEnzyme ActivationFeasibility StudiesMaleMatrix MetalloproteinasesMice, Inbred C57BLMice, TransgenicMolecular ImagingMultimodal ImagingPredictive Value of TestsRadiopharmaceuticalsRisk AssessmentRisk FactorsTime FactorsTomography, Emission-Computed, Single-PhotonTomography, X-Ray ComputedConceptsMicro-single photon emissionAngiotensin IIMatrix metalloproteinasesAortic diameterSuprarenal aortaCD68 expressionMMP activityPotential of MMPSaline-infused miceVessel wall inflammationAbdominal aortic aneurysmPrediction of outcomePhoton emissionRupture riskMurine AAAsAortic expansionRisk stratificationWall inflammationAortic aneurysmSpontaneous ruptureSmall aneurysmsMouse modelControl animalsTracer uptakeAbdominal aortic aneurysm (AAA) rupture risk
2004
Simvastatin Modulates Angiotensin II Signaling Pathway by Preventing Rac1-Mediated Upregulation of p27
ZENG L, XU H, CHEW TL, CHISHOLM R, SADEGHI MM, KANWAR YS, DANESH FR. Simvastatin Modulates Angiotensin II Signaling Pathway by Preventing Rac1-Mediated Upregulation of p27. Journal Of The American Society Of Nephrology 2004, 15: 1711-1720. PMID: 15213258, DOI: 10.1097/01.asn.0000129839.91567.68.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAngiotensin IIAnimalsBlotting, WesternCell Cycle ProteinsCell LineCyclin-Dependent Kinase Inhibitor p27Hydrogen PeroxideHydroxymethylglutaryl-CoA Reductase InhibitorsLeucineMicroscopy, ConfocalModels, BiologicalOxidation-ReductionPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRac1 GTP-Binding ProteinRatsSignal TransductionSimvastatinTransfectionTumor Suppressor ProteinsUp-RegulationConceptsP27 protein expressionAng IIAng II stimulationIntracellular H2O2 productionModulatory effectsMesangial cellsProtein expressionII stimulationCyclin-dependent kinase inhibitor p27P27 proteinEffect of simvastatinCell cycle levelSmall GTPAkt kinaseH2O2 productionRat mesangial cellsCholesterol-lowering propertiesAddition of mevalonateLipid attachmentDownstream activationRac1 activitySignaling pathwaysAkt activationIsoprenoid intermediatesGeranylgeranyl pyrophosphate