2021
Dynamic innate immune response determines susceptibility to SARS-CoV-2 infection and early replication kinetics
Cheemarla NR, Watkins TA, Mihaylova VT, Wang B, Zhao D, Wang G, Landry ML, Foxman EF. Dynamic innate immune response determines susceptibility to SARS-CoV-2 infection and early replication kinetics. Journal Of Experimental Medicine 2021, 218: e20210583. PMID: 34128960, PMCID: PMC8210587, DOI: 10.1084/jem.20210583.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAngiotensin-Converting Enzyme 2Case-Control StudiesChemokine CXCL10COVID-19Disease SusceptibilityFemaleGene Expression ProfilingHost-Pathogen InteractionsHumansImmunity, InnateInterferonsMaleMiddle AgedNasopharynxPicornaviridae InfectionsSARS-CoV-2Viral LoadVirus ReplicationConceptsSARS-CoV-2 infectionSARS-CoV-2 exposureSARS-CoV-2Interferon-stimulated genesUpper respiratory tractRespiratory tractEarly SARS-CoV-2 infectionDynamic innate immune responseViral replicationSARS-CoV-2 replicationPatient nasopharyngeal samplesInnate immune responseLow infectious doseViral loadNasopharyngeal samplesImmune responseInfectious doseISG responseAntiviral responseInfection progressionViral transmissionLevel correlatesInfectionISG inductionInitial replication
2020
Clinical impact of rapid influenza PCR in the adult emergency department on patient management, ED length of stay, and nosocomial infection rate
Peaper DR, Branson B, Parwani V, Ulrich A, Shapiro MJ, Clemons C, Campbell M, Owen M, Martinello RA, Landry ML. Clinical impact of rapid influenza PCR in the adult emergency department on patient management, ED length of stay, and nosocomial infection rate. Influenza And Other Respiratory Viruses 2020, 15: 254-261. PMID: 32851793, PMCID: PMC7902247, DOI: 10.1111/irv.12800.Peer-Reviewed Original ResearchConceptsEmergency departmentInfluenza PCRPatient managementClinical redesignED lengthInfluenza seasonED workflowPCR testingBed transfersAnti-influenza medicationsDiverse emergency departmentsHospital-acquired influenzaInfluenza-positive patientsHA influenzaNosocomial infection ratesDiagnosis of influenzaAdult emergency departmentAnti-viral therapyCommon presenting concernPost-intervention periodPre-post studyED LOSED patientsSignificant morbidityTherapeutic guidelinesHigh Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing
Landry ML, Topal JE, Estis J, Katzenbach P, Nolan N, Sandlund J. High Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing. Journal Of Clinical Microbiology 2020, 58: 10.1128/jcm.01629-19. PMID: 31776192, PMCID: PMC6989068, DOI: 10.1128/jcm.01629-19.Peer-Reviewed Original ResearchConceptsToxin enzyme immunoassayToxin A/BCell cytotoxicity neutralization assayEnzyme immunoassayStool samplesDifficile toxin assaysProspective clinical studyCytotoxicity neutralization assayCare algorithmChart reviewClinical studiesToxin ACytotoxin testingNeutralization assaysToxin assaysNegative agreementDiscordant samplesLaboratory algorithmTesting algorithmHigh agreementInfection diagnosticsGlutamate dehydrogenasePositive agreementImmunoassayAssays
2018
Adenovirus Type 4 Respiratory Infections among Civilian Adults, Northeastern United States, 2011–2015 - Volume 24, Number 2—February 2018 - Emerging Infectious Diseases journal - CDC
Kajon AE, Lamson DM, Bair CR, Lu X, Landry ML, Menegus M, Erdman DD, St. George K. Adenovirus Type 4 Respiratory Infections among Civilian Adults, Northeastern United States, 2011–2015 - Volume 24, Number 2—February 2018 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2018, 24: 201-209. PMID: 29350143, PMCID: PMC5782899, DOI: 10.3201/eid2402.171407.Peer-Reviewed Original ResearchConceptsAcute respiratory diseaseCivilian adultsInfectious Diseases journal - CDCUS military recruitsLong-term care facilitiesInfluenza-like illnessAdenovirus type 4Respiratory infectionsHuman adenovirus type 4Vaccination protocolsCancer patientsHAdV-4Respiratory diseasePhysician's officeCare facilitiesNursing homesMilitary recruitsVaccine strainYoung adultsType 4Causative agentAdultsLike variantsMilitary settingsDetailed molecular characterization
2016
Characterizing Patients with Very-Low-Level HIV Viremia: A Community-Based Study
Helou E, Shenoi S, Kyriakides T, Landry ML, Kozal M, Barakat LA. Characterizing Patients with Very-Low-Level HIV Viremia: A Community-Based Study. Journal Of The International Association Of Providers Of AIDS Care (JIAPAC) 2016, 16: 261-266. PMID: 27903948, PMCID: PMC5423832, DOI: 10.1177/2325957416680028.Peer-Reviewed Original ResearchConceptsVirologic failureViral load levelsPill burdenAlcohol useHuman immunodeficiency virus (HIV) careLow-level HIV viremiaUndetectable viral load levelsAntiretroviral regimen changesAntiretroviral therapy changesExperienced virologic failureLow-level viremiaIntravenous drug useSubset of patientsFrequent clinic visitsPoor clinical outcomePossible risk factorsHepatitis C virusHIV viremiaMore comorbiditiesNonfailure groupVirologic suppressionVirological failureCD4 countConsecutive chartsClinic visits
2014
Comparison of Simplexa Flu A/B & RSV PCR with Cytospin-Immunofluorescence and Laboratory-Developed TaqMan PCR in Predominantly Adult Hospitalized Patients
Landry ML, Ferguson D. Comparison of Simplexa Flu A/B & RSV PCR with Cytospin-Immunofluorescence and Laboratory-Developed TaqMan PCR in Predominantly Adult Hospitalized Patients. Journal Of Clinical Microbiology 2014, 52: 3057-3059. PMID: 24850350, PMCID: PMC4136128, DOI: 10.1128/jcm.00738-14.Peer-Reviewed Original Research
2013
Atypical hand-foot-and-mouth disease associated with coxsackievirus A6 infection
Lott JP, Liu K, Landry ML, Nix WA, Oberste MS, Bolognia J, King B. Atypical hand-foot-and-mouth disease associated with coxsackievirus A6 infection. Journal Of The American Academy Of Dermatology 2013, 69: 736-741. PMID: 24035209, PMCID: PMC5843477, DOI: 10.1016/j.jaad.2013.07.024.Peer-Reviewed Original ResearchConceptsCV-A6 infectionAtypical HFMDCV-A6Acute viral illnessArea of dermatitisCoxsackievirus A6 infectionDiagnostic laboratory evaluationMouth diseaseEnterovirus 71 infectionWidespread cutaneous lesionsReverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionAssociated systemic symptomsMucocutaneous lesionsViral illnessAtopic dermatitisCutaneous findingsCase seriesClinical featuresCutaneous lesionsEnterovirus infectionSystemic symptomsSingle institutionPolymerase chain reactionDiagnostic evaluationComparison of Simplexa HSV 1 & 2 PCR with Culture, Immunofluorescence, and Laboratory-Developed TaqMan PCR for Detection of Herpes Simplex Virus in Swab Specimens
Gitman MR, Ferguson D, Landry ML. Comparison of Simplexa HSV 1 & 2 PCR with Culture, Immunofluorescence, and Laboratory-Developed TaqMan PCR for Detection of Herpes Simplex Virus in Swab Specimens. Journal Of Clinical Microbiology 2013, 51: 3765-3769. PMID: 24006008, PMCID: PMC3889765, DOI: 10.1128/jcm.01413-13.Peer-Reviewed Original ResearchAdolescentAdultAgedAged, 80 and overChildChild, PreschoolClinical Laboratory TechniquesCosts and Cost AnalysisFemaleFluorescent Antibody TechniqueHerpes SimplexHumansInfantInfant, NewbornMaleMiddle AgedPolymerase Chain ReactionSensitivity and SpecificitySimplexvirusTime FactorsVirus CultivationYoung Adult
2010
Serum 25-Hydroxyvitamin D and the Incidence of Acute Viral Respiratory Tract Infections in Healthy Adults
Sabetta JR, DePetrillo P, Cipriani RJ, Smardin J, Burns LA, Landry ML. Serum 25-Hydroxyvitamin D and the Incidence of Acute Viral Respiratory Tract Infections in Healthy Adults. PLOS ONE 2010, 5: e11088. PMID: 20559424, PMCID: PMC2885414, DOI: 10.1371/journal.pone.0011088.Peer-Reviewed Original ResearchConceptsAcute viral respiratory tract infectionsViral respiratory tract infectionsRespiratory tract infectionsAcute respiratory tract infectionsTract infectionsD concentrationsVitamin DSerum concentrationsViral infectionHealthy adultsVitamin D supplementationD serum concentrationsBurden of illnessFuture interventional studiesIncidence of infectionSpecific viral infectionsLean body massPrevalence of influenzaPercentage of daysDark-skinned individualsD supplementationPregnant womenInterventional studyGeneral populationInfection
2009
Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus
Landry ML, Ferguson D. Cytospin-Enhanced Immunofluorescence and Impact of Sample Quality on Detection of Novel Swine Origin (H1N1) Influenza Virus. Journal Of Clinical Microbiology 2009, 48: 957-959. PMID: 20042632, PMCID: PMC2832425, DOI: 10.1128/jcm.01678-09.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsChildChild, PreschoolClinical Laboratory TechniquesFluorescent Antibody Technique, DirectHumansInfantInfant, NewbornInfluenza A Virus, H1N1 SubtypeInfluenza, HumanNasopharynxReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityVirologyYoung AdultConceptsNovel swine-origin influenza virusesSwine-origin influenza virusImmunofluorescence antibody testingReal-time reverse transcriptase PCRCycle threshold valuesPCR-positive resultsPediatric patientsCDC assayAntibody testingNasopharyngeal swabsReverse transcriptase-PCRSwine influenzaInfluenza virusTranscriptase-PCRPositive samplesPatientsPositive resultsInfluenzaSwabsCytospinsHerpes Simplex Type-2 Meningitis: Presentation and Lack of Standardized Therapy
Landry ML, Greenwold J, Vikram HR. Herpes Simplex Type-2 Meningitis: Presentation and Lack of Standardized Therapy. The American Journal Of Medicine 2009, 122: 688-691. PMID: 19559173, DOI: 10.1016/j.amjmed.2009.02.017.Peer-Reviewed Original ResearchConceptsHSV-2 meningitisGenital herpesHerpes simplex type 2Evidence of meningoencephalitisOral antiviral therapyRecurrent lymphocytic meningitisRetrospective observational studyTime of presentationSimplex type 2Optimal patient managementCerebrospinal fluid samplesSteroid injectionCesarean sectionLymphocytic meningitisOral therapyStandardized therapyAntiviral treatmentGenital symptomsIntravenous therapyRecurrent meningitisAntiviral therapySpinal stenosisGenital lesionsHSV-2Medical records
2008
An Outbreak of Concurrent Echovirus 30 and Coxsackievirus A1 Infections Associated with Sea Swimming among a Group of Travelers to Mexico
Begier EM, Oberste MS, Landry ML, Brennan T, Mlynarski D, Mshar PA, Frenette K, Rabatsky-Ehr T, Purviance K, Nepaul A, Nix WA, Pallansch MA, Ferguson D, Cartter ML, Hadler JL. An Outbreak of Concurrent Echovirus 30 and Coxsackievirus A1 Infections Associated with Sea Swimming among a Group of Travelers to Mexico. Clinical Infectious Diseases 2008, 47: 616-623. PMID: 18637756, DOI: 10.1086/590562.Peer-Reviewed Original ResearchConceptsEchovirus 30Enteroviral infectionNucleic acid amplification testingIllness onset datesUninfected control subjectsPoint-source exposureMultiple enterovirusesAseptic meningitisFrequent symptomsIllness onsetViral cultureAmplification testingControl subjectsCerebrospinal fluidHuman stoolInfectionNauseaConnecticut DepartmentStoolCoxsackievirus (CV) A1Public healthIllnessEnterovirusesMolecular amplificationGroups of travelersFalse negative PCR despite high levels of JC virus DNA in spinal fluid: Implications for diagnostic testing
Landry ML, Eid T, Bannykh S, Major E. False negative PCR despite high levels of JC virus DNA in spinal fluid: Implications for diagnostic testing. Journal Of Clinical Virology 2008, 43: 247-249. PMID: 18701345, PMCID: PMC2683978, DOI: 10.1016/j.jcv.2008.07.003.Peer-Reviewed Original ResearchConceptsProgressive multifocal leukoencephalopathyPolymerase chain reactionHerpes simplex virus polymerase chain reactionSpinal fluidCSF polymerase chain reactionNegative polymerase chain reactionFatal neurologic illnessesJC virus DNAFalse-negative polymerase chain reactionHigher positive resultsMultifocal leukoencephalopathyBrain biopsyNeurologic illnessDNA polymerase chain reactionNeurologic diseaseBlinded studyPCR testingDiagnostic testingVirus DNAReference laboratoryYoung mothersChain reactionNegative resultsDifferent virusesPositive resultsReal-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients
Landry ML, Cohen S, Ferguson D. Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients. Journal Of Clinical Virology 2008, 43: 148-151. PMID: 18639488, PMCID: PMC7173065, DOI: 10.1016/j.jcv.2008.06.006.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAntibodies, MonoclonalChildChild, PreschoolFluorescent Antibody Technique, DirectHospitalizationHumansImmunoassayInfantInfluenza A virusInfluenza B virusInfluenza, HumanMiddle AgedNasopharynxReagent Kits, DiagnosticReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityTaq PolymeraseTime FactorsVirus CultivationYoung AdultConceptsHospitalized patientsTaqMan RT-PCRRT-PCRInfluenza ARapid flu testsDiagnosis of influenzaInfluenza-positive samplesDetection of influenzaReal-time RT-PCRInfluenza BInfluenza diagnosisPatient managementReal-time PCRMore accurate testsPatientsBinaxTrue positive samplesRapid testClinical samplesPositive samplesFurther studiesHospitalAccurate testInfluenzaDiagnosisProspective Study of Human Metapneumovirus Detection in Clinical Samples by Use of Light Diagnostics Direct Immunofluorescence Reagent and Real-Time PCR
Landry ML, Cohen S, Ferguson D. Prospective Study of Human Metapneumovirus Detection in Clinical Samples by Use of Light Diagnostics Direct Immunofluorescence Reagent and Real-Time PCR. Journal Of Clinical Microbiology 2008, 46: 1098-1100. PMID: 18184854, PMCID: PMC2268359, DOI: 10.1128/jcm.01926-07.Peer-Reviewed Original Research
2007
Genotype Prevalence and Risk Factors for Severe Clinical Adenovirus Infection, United States 2004–2006
Gray GC, McCarthy T, Lebeck MG, Schnurr DP, Russell KL, Kajon AE, Landry ML, Leland DS, Storch GA, Ginocchio CC, Robinson CC, Demmler GJ, Saubolle MA, Kehl SC, Selvarangan R, Miller MB, Chappell JD, Zerr DM, Kiska DL, Halstead DC, Capuano AW, Setterquist SF, Chorazy ML, Dawson JD, Erdman DD. Genotype Prevalence and Risk Factors for Severe Clinical Adenovirus Infection, United States 2004–2006. Clinical Infectious Diseases 2007, 45: 1120-1131. PMID: 17918073, PMCID: PMC2064001, DOI: 10.1086/522188.Peer-Reviewed Original ResearchConceptsAdenovirus infectionIntensive care unit staySolid organ transplantationChronic disease conditionsAdenovirus isolatesUnited States 2004Adenovirus-positive specimensRisk factor modelingTyping methodsUnit stayRisk factorsAntigenic presentationChronic diseasesGenotype prevalenceClinical dataOrgan transplantationSevere diseaseRecent transplantationBone marrowBlinded comparisonDisease severityMilitary traineesSequence typing methodInfectionCancer diagnosisSerum IgA Antibodies to Epstein-Barr Virus (EBV) Early Lytic Antigens Are Present in Primary EBV Infection
Bhaduri-McIntosh S, Landry ML, Nikiforow S, Rotenberg M, El-Guindy A, Miller G. Serum IgA Antibodies to Epstein-Barr Virus (EBV) Early Lytic Antigens Are Present in Primary EBV Infection. The Journal Of Infectious Diseases 2007, 195: 483-492. PMID: 17230407, DOI: 10.1086/510916.Peer-Reviewed Original ResearchConceptsPrimary EBV infectionSerum IgA antibodiesIgA antibodiesEBV infectionLytic antigensPrimary infectionPrimary Epstein-Barr virus infectionHealthy EBV-seropositive individualsEpstein-Barr virus infectionEB nuclear antigenEBV-seropositive individualsViral capsid antigenAbsence of antibodiesFlow cytometry-based assayLytic EBV replicationCytometry-based assayBurkitt's lymphoma cellsCapsid antigenMucosal sitesEBV replicationIgM antibodiesVirus infectionInfectionLymphoma cellsSerum samples
2005
Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens
Landry ML, Garner R, Ferguson D. Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens. Journal Of Clinical Microbiology 2005, 43: 3136-3139. PMID: 16000425, PMCID: PMC1169110, DOI: 10.1128/jcm.43.7.3136-3139.2005.Peer-Reviewed Original ResearchConceptsNucleic acid sequence-based amplificationTime Nucleic Acid Sequence-Based AmplificationEV-positive samplesReal-time nucleic acid sequence-based amplificationDetection of enterovirusesEnterovirus RNAStool samplesCerebrospinal fluidNASBA assayClinical specimensEnterovirusesMolecular beacon technologyAssays
2004
Comparison of Binax NOW and Directigen for rapid detection of influenza A and B
Landry ML, Cohen S, Ferguson D. Comparison of Binax NOW and Directigen for rapid detection of influenza A and B. Journal Of Clinical Virology 2004, 31: 113-115. PMID: 15364266, DOI: 10.1016/j.jcv.2004.04.005.Peer-Reviewed Original Research
2003
Suboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin Immunofluorescence
Landry ML, Ferguson D. Suboptimal Detection of Influenza Virus in Adults by the Directigen Flu A+B Enzyme Immunoassay and Correlation of Results with the Number of Antigen-Positive Cells Detected by Cytospin Immunofluorescence. Journal Of Clinical Microbiology 2003, 41: 3407-3409. PMID: 12843105, PMCID: PMC165325, DOI: 10.1128/jcm.41.7.3407-3409.2003.Peer-Reviewed Original ResearchConceptsVirus infectionEnzyme immunoassayDirectigen FluParainfluenza virus infectionPositive EIA resultsRespiratory syncytial virusAntigen-positive cellsNasopharyngeal swabsSyncytial virusEmergency departmentInfluenza diagnosisDirect immunofluorescenceInfluenza virusVirology laboratoryEIA resultsNight shiftsInfectionInfluenzaImmunofluorescenceFluAdultsVirusCorrelation of resultsImmunoassayCells