2020
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
Vogels CBF, Brito AF, Wyllie AL, Fauver JR, Ott IM, Kalinich CC, Petrone ME, Casanovas-Massana A, Catherine Muenker M, Moore AJ, Klein J, Lu P, Lu-Culligan A, Jiang X, Kim DJ, Kudo E, Mao T, Moriyama M, Oh JE, Park A, Silva J, Song E, Takahashi T, Taura M, Tokuyama M, Venkataraman A, Weizman OE, Wong P, Yang Y, Cheemarla NR, White EB, Lapidus S, Earnest R, Geng B, Vijayakumar P, Odio C, Fournier J, Bermejo S, Farhadian S, Dela Cruz CS, Iwasaki A, Ko AI, Landry ML, Foxman EF, Grubaugh ND. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets. Nature Microbiology 2020, 5: 1299-1305. PMID: 32651556, PMCID: PMC9241364, DOI: 10.1038/s41564-020-0761-6.Peer-Reviewed Original ResearchConceptsSARS-CoV-2SARS-CoV-2 RTSevere acute respiratory syndrome coronavirusAcute respiratory syndrome coronavirusViral RNA copiesPublic health laboratoriesPublic health interventionsReverse transcription-PCR assaySARS-CoV-2 diagnostic testingDiagnostic assaysTranscription-PCR assaySARS-CoV-2 evolutionQuantitative reverse transcription-PCR assaysRapid diagnostic assaysHealth laboratoriesHealth interventionsDiagnostic testingRNA copiesPrimer-probe setsAssaysLow sensitivityCritical needAnalytical sensitivityHigh Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing
Landry ML, Topal JE, Estis J, Katzenbach P, Nolan N, Sandlund J. High Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing. Journal Of Clinical Microbiology 2020, 58: 10.1128/jcm.01629-19. PMID: 31776192, PMCID: PMC6989068, DOI: 10.1128/jcm.01629-19.Peer-Reviewed Original ResearchConceptsToxin enzyme immunoassayToxin A/BCell cytotoxicity neutralization assayEnzyme immunoassayStool samplesDifficile toxin assaysProspective clinical studyCytotoxicity neutralization assayCare algorithmChart reviewClinical studiesToxin ACytotoxin testingNeutralization assaysToxin assaysNegative agreementDiscordant samplesLaboratory algorithmTesting algorithmHigh agreementInfection diagnosticsGlutamate dehydrogenasePositive agreementImmunoassayAssays
2005
Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens
Landry ML, Garner R, Ferguson D. Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens. Journal Of Clinical Microbiology 2005, 43: 3136-3139. PMID: 16000425, PMCID: PMC1169110, DOI: 10.1128/jcm.43.7.3136-3139.2005.Peer-Reviewed Original ResearchConceptsNucleic acid sequence-based amplificationTime Nucleic Acid Sequence-Based AmplificationEV-positive samplesReal-time nucleic acid sequence-based amplificationDetection of enterovirusesEnterovirus RNAStool samplesCerebrospinal fluidNASBA assayClinical specimensEnterovirusesMolecular beacon technologyAssays
2000
A Standardized Plaque Reduction Assay for Determination of Drug Susceptibilities of Cytomegalovirus Clinical Isolates
Landry M, Stanat S, Biron K, Brambilla D, Britt W, Jokela J, Chou S, Drew W, Erice A, Gilliam B, Lurain N, Manischewitz J, Miner R, Nokta M, Reichelderfer P, Spector S, Weinberg A, Yen-Lieberman B, Crumpacker C, Group T. A Standardized Plaque Reduction Assay for Determination of Drug Susceptibilities of Cytomegalovirus Clinical Isolates. Antimicrobial Agents And Chemotherapy 2000, 44: 688-692. PMID: 10681339, PMCID: PMC89747, DOI: 10.1128/aac.44.3.688-692.2000.Peer-Reviewed Original Research