1994
Effect of insulin on rat heart and skeletal muscle phenylalanyl-tRNA labeling and protein synthesis in vivo
Young LH, Stirewalt W, McNulty PH, Revkin JH, Barrett EJ. Effect of insulin on rat heart and skeletal muscle phenylalanyl-tRNA labeling and protein synthesis in vivo. American Journal Of Physiology 1994, 267: e337-e342. PMID: 8074214, DOI: 10.1152/ajpendo.1994.267.2.e337.Peer-Reviewed Original ResearchConceptsMuscle protein synthesisAcid-soluble poolFemoral venous plasmaSkeletal muscleSkeletal muscle protein synthesisEffect of insulinMuscle protein hydrolysateProtein synthesisContinuous infusionVenous plasmaHyperinsulinemic clampSaline infusionAnesthetized ratsArterial plasmaRat heartInsulin effectInsulinHormonal regulationHeartInfusionMuscleSpecific activityMinVivo measurementsLabeling
1993
Response of rat heart and skeletal muscle protein in vivo to insulin and amino acid infusion
McNulty PH, Young LH, Barrett EJ. Response of rat heart and skeletal muscle protein in vivo to insulin and amino acid infusion. American Journal Of Physiology 1993, 264: e958-e965. PMID: 8333520, DOI: 10.1152/ajpendo.1993.264.6.e958.Peer-Reviewed Original ResearchConceptsAmino acid infusionMuscle protein synthesisContinuous infusionAcid infusionNet muscle protein synthesisSaline-treated ratsSkeletal muscle protein synthesisInfusion of insulinPlasma specific activitySkeletal muscle proteinsProtein synthesisAmino acid solutionMature ratsInfusionRat heartInsulinBolusTracer infusionHeart muscleSkeletal muscleTracer infusion methodRatsPhysiological concentrationsAmino acids
1987
An isotopic method for measurement of muscle protein synthesis and degradation in vivo
Barrett EJ, Revkin JH, Young LH, Zaret BL, Jacob R, Gelfand RA. An isotopic method for measurement of muscle protein synthesis and degradation in vivo. Biochemical Journal 1987, 245: 223-228. PMID: 3663148, PMCID: PMC1148103, DOI: 10.1042/bj2450223.Peer-Reviewed Original ResearchConceptsNet amino acid releaseFemoral venous plasmaTissue blood flowAmino acid releaseMuscle protein synthesisHindlimb flowTissue protein degradationSpecific radioactivityContinuous infusionUptake of leucineMuscle bedArterial circulationClinical studiesPlasma concentrationsDog hindlimbBlood flowProtein synthesisAcid releaseHindlimbProtein turnoverTissue proteinsPhenylalanine incorporationRemoval of tracerMuscle tissueDogs