Featured Publications
Malaria Resilience in South America: Epidemiology, Vector Biology, and Immunology Insights from the Amazonian International Center of Excellence in Malaria Research Network in Peru and Brazil
Torres K, Ferreira MU, Castro MC, Escalante AA, Conn JE, Villasis E, da Silva Araujo M, Almeida G, Rodrigues PT, Corder RM, Fernandes ARJ, Calil PR, Ladeia WA, Garcia-Castillo SS, Gomez J, do Valle Antonelli LR, Gazzinelli RT, Golenbock DT, Llanos-Cuentas A, Gamboa D, Vinetz JM. Malaria Resilience in South America: Epidemiology, Vector Biology, and Immunology Insights from the Amazonian International Center of Excellence in Malaria Research Network in Peru and Brazil. American Journal Of Tropical Medicine And Hygiene 2022, 107: 168-181. PMID: 36228921, PMCID: PMC9662219, DOI: 10.4269/ajtmh.22-0127.Peer-Reviewed Original ResearchConceptsImmune responseMalaria epidemiologyMalaria controlImportant public health priorityHuman immune responsePublic health priorityWestern Brazilian Amazon regionHealth priorityHuman reservoirSociodemographic featuresMalaria researchEpidemiologyMultidisciplinary approachRapid reemergenceVector biologyElimination strategyResearch NetworkMalaria vectorsInternational CentreFurther researchBrazilian Amazon regionSouth AmericaMalariaImmunologyResponse
2024
Recurrence patterns and evolution of submicroscopic and asymptomatic Plasmodium vivax infections in malaria-endemic areas of the Peruvian Amazon
Castillo S, Alvarez C, Rosas-Aguirre Á, Acosta C, Corder R, Gómez J, Guzmán M, Speybroeck N, Llanos-Cuentas A, Castro M, Rosanas-Urgell A, Ferreira M, Vinetz J, Gamboa D, Torres K. Recurrence patterns and evolution of submicroscopic and asymptomatic Plasmodium vivax infections in malaria-endemic areas of the Peruvian Amazon. PLOS Neglected Tropical Diseases 2024, 18: e0012566. PMID: 39480785, PMCID: PMC11527163, DOI: 10.1371/journal.pntd.0012566.Peer-Reviewed Original ResearchConceptsRisk of P. vivax infectionPlasmodium vivax malaria transmissionFactors associated with recurrenceVivax malaria transmissionPlasmodium vivax infectionMalaria-endemic areasPopulation-based cohort studyFrequency of recurrenceApplication of molecular diagnostic methodsMalaria recurrenceMalaria infectionMalaria transmissionVivax infectionRecurrence rateMolecular diagnostic methodsRecurrence riskConsequences of prolonged exposureFollow-upCohort studyEndemic settingsRecurrenceAsymptomatic infectionAsymptomatic casesInfectionInitial infectionGenomic surveillance of malaria parasites in an indigenous community in the Peruvian Amazon
Cabrera-Sosa L, Nolasco O, Kattenberg J, Fernandez-Miñope C, Valdivia H, Barazorda K, Arévalo de los Rios S, Rodriguez-Ferrucci H, Vinetz J, Rosanas-Urgell A, Van geertruyden J, Gamboa D, Delgado-Ratto C. Genomic surveillance of malaria parasites in an indigenous community in the Peruvian Amazon. Scientific Reports 2024, 14: 16291. PMID: 39009685, PMCID: PMC11250820, DOI: 10.1038/s41598-024-66925-x.Peer-Reviewed Original ResearchConceptsPassive case detectionPf parasitesPersistent malaria transmissionPfhrp2/3 gene deletionsDeep sequencing assaySulfadoxine-pyrimethamineMalaria casesMalaria eliminationPlasmodium vivaxMalaria parasitesMalaria transmissionP. falciparumResistance mutationsImported infectionsMalariaPopulation genetic indicesSequencing assayCase detectionGene deletionPV populationResistance markersGenetic diversityPeruvian AmazonGenomic surveillanceGenetic indicesEffect of spatiotemporal variables on abundance, biting activity and parity of Nyssorhynchus darlingi (Diptera: Culicidae) in peri-Iquitos, Peru
Bickersmith S, Saavedra M, Prussing C, Lange R, Morales J, Alava F, Vinetz J, Gamboa D, Moreno M, Conn J. Effect of spatiotemporal variables on abundance, biting activity and parity of Nyssorhynchus darlingi (Diptera: Culicidae) in peri-Iquitos, Peru. Malaria Journal 2024, 23: 112. PMID: 38641572, PMCID: PMC11031940, DOI: 10.1186/s12936-024-04940-z.Peer-Reviewed Original ResearchConceptsMalaria transmissionBreeding site availabilityPresence of Plasmodium vivaxDry seasonBiting behaviorRisk of malaria transmissionMalaria-endemic regionsHuman malaria casesPlasmodium falciparum sporozoitesRainy seasonCommunity typesNyssorhynchus darlingiRates of parityReal-time PCRAmazonian PeruP. vivaxMalaria casesPlasmodium vivaxHuman landing catchesP. falciparumEffect of seasonMosquito behaviorConclusionsThese dataDaily survivalDarlingiA prospective cohort study linking migration, climate, and malaria risk in the Peruvian Amazon – CORRIGENDUM
Gunderson A, Recalde-Coronel C, Zaitchik B, Yori P, Pinedo S, Olortegui M, Kosek M, Vinetz J, Pan W. A prospective cohort study linking migration, climate, and malaria risk in the Peruvian Amazon – CORRIGENDUM. Epidemiology And Infection 2024, 152: e5. PMID: 38214060, PMCID: PMC10789972, DOI: 10.1017/s0950268824000025.Peer-Reviewed Original Research
2023
A prospective cohort study linking migration, climate, and malaria risk in the Peruvian Amazon
Gunderson A, Recalde-Coronel C, Zaitchik B, Yori P, Pinedo S, Olortegui M, Kosek M, Vinetz J, Pan W. A prospective cohort study linking migration, climate, and malaria risk in the Peruvian Amazon. Epidemiology And Infection 2023, 151: e202. PMID: 38031496, PMCID: PMC10753477, DOI: 10.1017/s0950268823001838.Peer-Reviewed Original ResearchMutations Linked to Insecticide Resistance Not Detected in the Ace-1 or VGSC Genes in Nyssorhynchus darlingi from Multiple Localities in Amazonian Brazil and Peru
Bickersmith S, Jurczynski J, Sallum M, Chaves L, Bergo E, Rodriguez G, Morante C, Rios C, Saavedra M, Alava F, Gamboa D, Vinetz J, Conn J. Mutations Linked to Insecticide Resistance Not Detected in the Ace-1 or VGSC Genes in Nyssorhynchus darlingi from Multiple Localities in Amazonian Brazil and Peru. Genes 2023, 14: 1892. PMID: 37895241, PMCID: PMC10606710, DOI: 10.3390/genes14101892.Peer-Reviewed Original ResearchConceptsIndoor residual sprayMalaria endemic localitiesLow-intensity resistanceResistance mechanismsMalaria hotspotsCommon interventionInsecticidal netsMain resistance mechanismMalaria transmissionGenotypic detectionCodon 280Resistance mutationsEndemic localitiesPrimary malaria vectorVoltage-gated sodium channel geneResidual spraySodium channel geneChannel genesNon-synonymous mutationsACEPresent studyMalaria vectorsInterventionMutationsS6 segmentNatural Infection of Nyssorhynchus darlingi and Nyssorhynchus benarrochi B with Plasmodium during the Dry Season in the Understudied Low-Transmission Setting of Datem del Marañon Province, Amazonian Peru
Conn J, Bickersmith S, Saavedra M, Morales J, Alava F, Rodriguez G, del Aguila Morante C, Tong C, Alvarez-Antonio C, Huanahui J, Vinetz J, Gamboa D. Natural Infection of Nyssorhynchus darlingi and Nyssorhynchus benarrochi B with Plasmodium during the Dry Season in the Understudied Low-Transmission Setting of Datem del Marañon Province, Amazonian Peru. American Journal Of Tropical Medicine And Hygiene 2023, 109: 288-295. PMID: 37364858, PMCID: PMC10397451, DOI: 10.4269/ajtmh.23-0058.Peer-Reviewed Original ResearchConceptsLow transmission settingsAnnual parasite indexEntomological inoculation rateHuman biting rateHuman landing catchesMinistry of HealthMalaria hotspotsInfective bitesPlasmodium vivaxMalaria transmissionLanding catchesPlasmodium falciparumMalaria researchNatural infectionInoculation rateNyssorhynchus darlingiBiting rateRiverine villagesParasite indexBiteVector control unitsDarlingiLoreto DepartmentInfectionVivax
2022
Geographical distribution and genetic characterization of pfhrp2 negative Plasmodium falciparum parasites in the Peruvian Amazon
Bendezu J, Torres K, Villasis E, Incardona S, Bell D, Vinetz J, Gamboa D. Geographical distribution and genetic characterization of pfhrp2 negative Plasmodium falciparum parasites in the Peruvian Amazon. PLOS ONE 2022, 17: e0273872. PMID: 36413547, PMCID: PMC9681099, DOI: 10.1371/journal.pone.0273872.Peer-Reviewed Original ResearchEffect of out-of-village working activities on recent malaria exposure in the Peruvian Amazon using parametric g-formula
Carrasco-Escobar G, Rosado J, Nolasco O, White M, Mueller I, Castro M, Rodriguez-Ferruci H, Gamboa D, Llanos-Cuentas A, Vinetz J, Benmarhnia T. Effect of out-of-village working activities on recent malaria exposure in the Peruvian Amazon using parametric g-formula. Scientific Reports 2022, 12: 19144. PMID: 36351988, PMCID: PMC9645738, DOI: 10.1038/s41598-022-23528-8.Peer-Reviewed Original ResearchConceptsRecent malaria exposureMalaria exposurePopulation-based cross-sectional studyCommunity-level transmissionMalaria elimination strategiesCross-sectional studyPotential health benefitsWorking activitiesRisk differenceOccupational interventionsMalaria transmissionAdult populationOccupational activitiesHeterogeneous patternUnexposed populationIndividual riskHealth benefitsPopulation subgroupsRural areasElimination strategyVillage workersMean outcomeIntervention scenariosExposurePopulationEvidence-Based Malaria Control and Elimination in the Amazon: Input from the International Center of Excellence in Malaria Research Network in Peru and Brazil
Ferreira MU, Gamboa D, Torres K, Rodriguez-Ferrucci H, Soto-Calle VE, Pardo K, Fontoura PS, Tomko SS, Gazzinelli RT, Conn JE, Castro MC, Llanos-Cuentas A, Vinetz JM. Evidence-Based Malaria Control and Elimination in the Amazon: Input from the International Center of Excellence in Malaria Research Network in Peru and Brazil. American Journal Of Tropical Medicine And Hygiene 2022, 107: 160-167. PMID: 36228907, PMCID: PMC9662230, DOI: 10.4269/ajtmh.21-1272.Peer-Reviewed Original ResearchConceptsMalaria controlAsymptomatic Plasmodium infectionsPredominant malaria parasitePublic health decision makersMalaria control strategiesPublic health implicationsIndoor residual sprayingBetter malaria controlHealth decision makersVector control measuresPlasmodium infectionInfectious reservoirMalaria burdenPlasmodium vivaxBed netsPublic health policymakingElimination programMalaria parasitesResidual sprayingMalaria researchHealth implicationsNyssorhynchus darlingiDiagnostic methodsHealth policymakingResearch NetworkEtiologies of Acute Undifferentiated Febrile Illnesses in and near Iquitos from 1993 to 1999 in the Amazon River Basin of Peru
Watts DM, Russell KL, Wooster MT, Sharp TW, Morrison AC, Kochel TJ, Bautista CT, Block K, Guevara C, Aguilar P, Palermo PM, Calampa C, Porter KR, Hayes CG, Weaver SC, de Rosa AT, Vinetz JM, Shope RE, Gotuzzo E, Guzman H, Tesh RB. Etiologies of Acute Undifferentiated Febrile Illnesses in and near Iquitos from 1993 to 1999 in the Amazon River Basin of Peru. American Journal Of Tropical Medicine And Hygiene 2022, 107: 1114-1128. PMID: 36162442, PMCID: PMC9709010, DOI: 10.4269/ajtmh.22-0259.Peer-Reviewed Original ResearchConceptsFebrile illnessAcute undifferentiated febrile illnessUndifferentiated febrile illnessFebrile patientsAcute leptospirosisFebrile casesAsymptomatic infectionIgG antibodiesHealth burdenHealth clinicsClinical dataBlood samplesMicroscopic agglutinationDengue virusPatientsEncephalitis virusBlood smearsHuman morbidityLeptospira sppMalariaFurther studiesArbovirusesHealth impactsBaseline knowledgeEtiologyEpidemiological characteristics of P. vivax asymptomatic infections in the Peruvian Amazon
Villasis E, Castillo S, Guzman M, Torres J, Gomez J, Garro K, Cordova AM, Reategui C, Abanto C, Vinetz J, Gamboa D, Torres K. Epidemiological characteristics of P. vivax asymptomatic infections in the Peruvian Amazon. Frontiers In Cellular And Infection Microbiology 2022, 12: 901423. PMID: 36118037, PMCID: PMC9471197, DOI: 10.3389/fcimb.2022.901423.Peer-Reviewed Original ResearchConceptsCase-control studyAntimalarial treatmentMalaria symptomsHematological parametersControl individualsCommon malaria symptomsNegative microscopy resultsHistory of comorbiditiesMalaria control measuresClinical immunityMalaria microscopyAsymptomatic infectionMalaria infectionEpidemiological characteristicsChronic infectionMost infectionsMalaria eliminationDay 21Positive qPCR resultsInfected individualsPopulation screeningSYM groupInfectionQPCR resultsLast monthMalaria transmission structure in the Peruvian Amazon through antibody signatures to Plasmodium vivax
Rosado J, Carrasco-Escobar G, Nolasco O, Garro K, Rodriguez-Ferruci H, Guzman-Guzman M, Llanos-Cuentas A, Vinetz JM, Nekkab N, White MT, Mueller I, Gamboa D. Malaria transmission structure in the Peruvian Amazon through antibody signatures to Plasmodium vivax. PLOS Neglected Tropical Diseases 2022, 16: e0010415. PMID: 35533146, PMCID: PMC9119515, DOI: 10.1371/journal.pntd.0010415.Peer-Reviewed Original ResearchConceptsSerological exposure markersP. vivaxMalaria transmissionAntibody levelsRecent exposureRoutine malaria surveillanceHigher antibody levelsCross-sectional surveyLight microscopySeropositive casesAntibody signaturesPast infectionRisk factorsPrevalence surveyHigher oddsMalaria surveillanceExposure markersElimination effortsSignificant associationSocio-DemographicEpidemiological analysisSerological surveillanceParticular epidemiologyLocal transmissionVivax
2021
Integrating Parasitological and Entomological Observations to Understand Malaria Transmission in Riverine Villages in the Peruvian Amazon
Rosas-Aguirre A, Moreno M, Moreno-Gutierrez D, Llanos-Cuentas A, Saavedra M, Contreras-Mancilla J, Barboza J, Alava F, Aguirre K, Carrasco G, Prussing C, Vinetz J, Conn JE, Speybroeck N, Gamboa D. Integrating Parasitological and Entomological Observations to Understand Malaria Transmission in Riverine Villages in the Peruvian Amazon. The Journal Of Infectious Diseases 2021, 223: s99-s110. PMID: 33906225, PMCID: PMC8079135, DOI: 10.1093/infdis/jiaa496.Peer-Reviewed Original ResearchConceptsMalaria prevalenceMalaria transmissionRiverine villagesMalaria risk factorsMalaria transmission dynamicsSubmicroscopic infectionsLocal epidemiologyPolymerase chain reactionIncidence rateRisk factorsHigh prevalenceMalaria casesTreat interventionMalaria strategyPlasmodium vivaxPrevalenceChain reactionTransmission dynamicsAdult AnophelesHigh heterogeneityHeterogeneity in response to serological exposure markers of recent Plasmodium vivax infections in contrasting epidemiological contexts
Rosado J, White MT, Longley RJ, Lacerda M, Monteiro W, Brewster J, Sattabongkot J, Guzman-Guzman M, Llanos-Cuentas A, Vinetz JM, Gamboa D, Mueller I. Heterogeneity in response to serological exposure markers of recent Plasmodium vivax infections in contrasting epidemiological contexts. PLOS Neglected Tropical Diseases 2021, 15: e0009165. PMID: 33591976, PMCID: PMC7909627, DOI: 10.1371/journal.pntd.0009165.Peer-Reviewed Original ResearchConceptsSerological exposure markersBlood-stage infectionLow transmission settingsP. vivax infectionPlasmodium vivax infectionHigh transmission settingsVivax infectionTransmission settingsAntibody titersAntibody responseExposure markersDifferent transmission intensitiesIgG responsesSerological markersPrevious infectionReceiver Operating Characteristic (ROC) analysisEndemic settingsMalaria eliminationPeruvian cohortOperating characteristics analysisP. vivaxMalaria controlRecent exposureInfectionEpidemiological contextNew Records of Anopheles benarrochi B (Diptera: Culicidae) in Malaria Hotspots in the Amazon Regions of Ecuador and Peru
Viteri D, Herrera-Varela M, Albuja M, Quiroga C, Diaz G, del Aguila Morante C, Ramirez D, Vinetz J, Bickersmith S, Conn J. New Records of Anopheles benarrochi B (Diptera: Culicidae) in Malaria Hotspots in the Amazon Regions of Ecuador and Peru. Journal Of Medical Entomology 2021, 58: 1234-1240. PMID: 33511394, PMCID: PMC8349109, DOI: 10.1093/jme/tjaa293.Peer-Reviewed Original Research
2018
Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon
Moreno M, Tong-Rios C, Orjuela-Sanchez P, Carrasco-Escobar G, Campo B, Gamboa D, Winzeler EA, Vinetz JM. Continuous Supply of Plasmodium vivax Sporozoites from Colonized Anopheles darlingi in the Peruvian Amazon. ACS Infectious Diseases 2018, 4: 541-548. PMID: 29465219, PMCID: PMC5902790, DOI: 10.1021/acsinfecdis.7b00195.Peer-Reviewed Original ResearchConceptsPlasmodium vivax sporozoitesDonor seraPlasmodium vivax liver stagesStandard membrane feeding assaysMalaria-endemic settingsMembrane feeding assaysSporozoite productionInfectious blood mealInfected subjectsNaïve serumBlood-fed mosquitoesLiver stagesDonor bloodBlood samplesVaccine developmentMosquito midgutSerumBlood mealBloodMosquito survivalSporozoitesMosquitoesAnophelesAnticoagulantsInfection
2016
Epidemiology of Plasmodium vivax Malaria in Peru
Rosas-Aguirre A, Gamboa D, Manrique P, Conn JE, Moreno M, Lescano AG, Sanchez JF, Rodriguez H, Silva H, Llanos-Cuentas A, Vinetz JM. Epidemiology of Plasmodium vivax Malaria in Peru. American Journal Of Tropical Medicine And Hygiene 2016, 95: 133-144. PMID: 27799639, PMCID: PMC5201219, DOI: 10.4269/ajtmh.16-0268.Peer-Reviewed Original ResearchConceptsMalaria transmissionP. vivaxMalaria controlPlasmodium falciparum malaria casesMicroscopy-confirmed casesP. vivax malaria transmissionFalciparum malaria casesPlasmodium vivax malariaVivax malaria transmissionPublic health problemGlucose-6-phosphate dehydrogenase deficiencyFuture malaria controlImplementation of surveillanceCombination therapyVivax malariaClinical trialsMalaria casesPlasmodium vivaxElimination effortsHealth problemsP. falciparumMosquito transmissionDehydrogenase deficiencyVivaxOngoing surveillance activities
2000
Detection of pathogenic Leptospira spp. infections among mammals captured in the Peruvian Amazon basin region.
Bunnell J, Hice C, Watts D, Montrueil V, Tesh R, Vinetz J. Detection of pathogenic Leptospira spp. infections among mammals captured in the Peruvian Amazon basin region. American Journal Of Tropical Medicine And Hygiene 2000, 63: 255-8. PMID: 11421373, DOI: 10.4269/ajtmh.2000.63.255.Peer-Reviewed Original Research