2017
Mechanisms of bile acid mediated inflammation in the liver
Li M, Cai SY, Boyer JL. Mechanisms of bile acid mediated inflammation in the liver. Molecular Aspects Of Medicine 2017, 56: 45-53. PMID: 28606651, PMCID: PMC5662014, DOI: 10.1016/j.mam.2017.06.001.Peer-Reviewed Original ResearchConceptsLiver injuryBile acidsCholestatic animal modelsCauses of cholestasisCholestatic liver injuryInnate immune cellsBiliary injuryNeutrophil recruitmentBile flowImmune cellsEffective therapyInflammatory responseAnimal modelsMolecular mediatorsCholestasisInjuryNovel targetLiverElevated levelsPathological processesMolecular mechanismsHepatocytesInflammationPatientsPathogenesis
2016
CFTR-associated ligand is a negative regulator of Mrp2 expression
Li M, Soroka CJ, Harry K, Boyer JL. CFTR-associated ligand is a negative regulator of Mrp2 expression. American Journal Of Physiology - Cell Physiology 2016, 312: c40-c46. PMID: 27834195, PMCID: PMC5283898, DOI: 10.1152/ajpcell.00100.2016.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCells, CulturedChlorocebus aethiopsCOS CellsDown-RegulationGene Expression RegulationGolgi Matrix ProteinsHepatocytesHumansMaleMembrane ProteinsMembrane Transport ProteinsMiceMultidrug Resistance-Associated Protein 2Multidrug Resistance-Associated ProteinsRatsRats, Sprague-DawleySignal TransductionConceptsPull-down assaysGST pull-down assaysCOOH-terminal PDZNegative regulatorCotransfected COS-7 cellsGlutathione S-transferase fusion proteinS-transferase fusion proteinATP-binding cassette (ABC) transportersTrans-Golgi networkCystic fibrosis transmembrane conductance regulatorProtein-protein interactionsExchanger regulatory factor 1Fibrosis transmembrane conductance regulatorStreptavidin pull-down assaysTransmembrane conductance regulatorCOS-7 cellsRegulatory factor 1PDZ domainCell surface expressionPosttranscriptional regulationTransmembrane proteinPlasma membraneLLC-PK1 cellsCassette transportersCOS-7
2011
Drug‐induced cholestasis
Padda MS, Sanchez M, Akhtar AJ, Boyer JL. Drug‐induced cholestasis. Hepatology 2011, 53: 1377-1387. PMID: 21480339, PMCID: PMC3089004, DOI: 10.1002/hep.24229.Peer-Reviewed Original Research
2010
Cholestasis: Genetic and Acquired
Boyer J. Cholestasis: Genetic and Acquired. Seminars In Liver Disease 2010, 30: 113-115. PMID: 20422493, DOI: 10.1055/s-0030-1253220.Peer-Reviewed Original ResearchConceptsBile salt export pumpCholestatic liver diseaseMultidrug resistance protein 3Cholestatic liver injuryLiver diseaseBile acidsBile salt transportersIssue of SeminarsLiver injuryCholestatic disordersBile flowSulfate conjugatesSodium taurocholate co-transporting polypeptideBile duct-cannulated animalsBile formationExport pumpProtein 3Bile acid-independent bile flowSalt transportersTaurocholate co-transporting polypeptideOrganic solute transporters alphaDependent bile salt transporterApical sodium-dependent bile salt transporterFamilial intrahepatic cholestasis-1Drug-induced cholestasis
2002
Mechanisms of Hepatic Transport of Drugs: Implications for Cholestatic Drug Reactions
Bohan A, Boyer J. Mechanisms of Hepatic Transport of Drugs: Implications for Cholestatic Drug Reactions. Seminars In Liver Disease 2002, 22: 123-136. PMID: 12016544, DOI: 10.1055/s-2002-30099.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCholestasisDisease Models, AnimalHumansLiverPharmaceutical PreparationsPolymorphism, GeneticXenobioticsConceptsDrug-induced cholestasisCholestatic drug reactionDrug reactionsDrug transporter expressionNumber of drugsBiliary excretionCanalicular transport proteinsAnimal modelsHepatic uptakeHepatobiliary transportersCholestasisTransporter expressionHepatic transportGenetic polymorphismsNormal functionDrugsMajor determinantForeign compoundsStudy of mechanismsTransportersExcretion
2001
Adaptive regulation of bile salt transporters in kidney and liver in obstructive cholestasis in the rat
Lee J, Azzaroli F, Wang L, Soroka C, Gigliozzi A, Setchell K, Kramer W, Boyer J. Adaptive regulation of bile salt transporters in kidney and liver in obstructive cholestasis in the rat. Gastroenterology 2001, 121: 1473-1484. PMID: 11729126, DOI: 10.1053/gast.2001.29608.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAnimalsBile Acids and SaltsCarrier ProteinsCholestasisCommon Bile DuctFluorescent Antibody TechniqueKidneyLigationLiverMaleMicrovilliMitochondrial ProteinsOrganic Anion Transporters, Sodium-DependentRatsRats, Sprague-DawleyRibosomal ProteinsRNA, MessengerSaccharomyces cerevisiae ProteinsSymportersConceptsBile salt excretionCommon bile duct ligationBile salt transportersBile duct ligationSalt excretionObstructive cholestasisSalt transportersDuct ligationCommon bile duct obstructionKidney 14 daysBile duct obstructionBile salt transport proteinsSerum bile saltsBrush border membrane vesiclesProtein 2 expressionBile salt transportSodium-dependent uptakeExtrahepatic pathwaysLiver injuryDuct obstructionTissue immunofluorescenceBorder membrane vesiclesTransporter messenger RNAExtrahepatic tissuesTotal liverCl−-dependent secretory mechanisms in isolated rat bile duct epithelial units
Singh S, Mennone A, Gigliozzi A, Fraioli F, Boyer J. Cl−-dependent secretory mechanisms in isolated rat bile duct epithelial units. AJP Gastrointestinal And Liver Physiology 2001, 281: g438-g446. PMID: 11447024, DOI: 10.1152/ajpgi.2001.281.2.g438.Peer-Reviewed Original ResearchMeSH KeywordsAmmonium ChlorideAnimalsBariumBicarbonatesBile Ducts, IntrahepaticBumetanideCarbon DioxideCarrier ProteinsCell PolarityCells, CulturedChloridesColforsinEpithelial CellsHydrogen-Ion ConcentrationIon TransportMaleModels, BiologicalRatsRats, Sprague-DawleySodiumSodium-Potassium-Chloride SymportersConceptsCO2/HCOFluid secretionCotransport activityIntrahepatic bile duct epitheliumBile duct epitheliumDependent fluid secretionHigh intracellular Cl(-) concentrationIntracellular Cl- concentrationBile duct unitsApical Cl(-) secretionNH entryInitial acidification rateIntracellular cAMP concentrationNH4Cl exposureDuct epitheliumInhibitor bumetanideChannel inhibitorsCl(-) secretionCanalicular bileDuct unitsSecretionDependent secretionSecretory mechanismBumetanideForskolinExpression cloning of two genes that together mediate organic solute and steroid transport in the liver of a marine vertebrate
Wang W, Seward D, Li L, Boyer J, Ballatori N. Expression cloning of two genes that together mediate organic solute and steroid transport in the liver of a marine vertebrate. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 9431-9436. PMID: 11470901, PMCID: PMC55438, DOI: 10.1073/pnas.161099898.Peer-Reviewed Original Research
2000
ATP-dependent GSH and glutathioneS-conjugate transport in skate liver: role of an Mrp functional homologue
Rebbeor J, Connolly G, Henson J, Boyer J, Ballatori N. ATP-dependent GSH and glutathioneS-conjugate transport in skate liver: role of an Mrp functional homologue. AJP Gastrointestinal And Liver Physiology 2000, 279: g417-g425. PMID: 10915652, DOI: 10.1152/ajpgi.2000.279.2.g417.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAnion Transport ProteinsAnionsATP-Binding Cassette TransportersBileBiological TransportCarrier ProteinsCell MembraneEnzyme InhibitorsGlutathioneLiverMaleOxidation-ReductionProtein BindingRatsRats, Sprague-DawleySkates, FishSubstrate SpecificityTaurocholic AcidTritiumConceptsATP-dependent transportLiver membrane vesiclesATP-dependent GSH transportMembrane vesiclesTransport activityATP-independent mechanismGlutathione-conjugate transportFunctional homologueMammalian cellsDNP-SG uptakePlasma membraneHomologous proteinsSkate liverGSH transportLow-affinity pathwayLittle skateMRP familyMultidrug resistance-associated protein 1GSH effluxFunctional assaysProtein 1First direct evidenceProtein bandsDirect evidenceHomologuesExpression of the bile salt export pump is maintained after chronic cholestasis in the rat
Lee J, Trauner M, Soroka C, Stieger B, Meier P, Boyer J. Expression of the bile salt export pump is maintained after chronic cholestasis in the rat. Gastroenterology 2000, 118: 163-172. PMID: 10611165, DOI: 10.1016/s0016-5085(00)70425-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsATP Binding Cassette Transporter, Subfamily B, Member 1ATP Binding Cassette Transporter, Subfamily B, Member 11ATP-Binding Cassette TransportersBile Acids and SaltsBile DuctsCarrier ProteinsCholestasisChronic DiseaseDrug Resistance, MultipleEthinyl EstradiolLigationLipopolysaccharidesMembrane ProteinsMultidrug Resistance-Associated ProteinsOrganic Anion Transporters, Sodium-DependentRatsRats, Sprague-DawleyRNA, MessengerSymporters
1999
Carrier-mediated uptake of lucifer yellow in skate and rat hepatocytes: a fluid-phase marker revisited
Ballatori N, Hager D, Nundy S, Miller D, Boyer J. Carrier-mediated uptake of lucifer yellow in skate and rat hepatocytes: a fluid-phase marker revisited. American Journal Of Physiology 1999, 277: g896-g904. PMID: 10516157, DOI: 10.1152/ajpgi.1999.277.4.g896.Peer-Reviewed Original Research
1998
Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver
Cho W, Mennone A, Boyer J. Intracellular pH regulation in bombesin-stimulated secretion in isolated bile duct units from rat liver. American Journal Of Physiology 1998, 275: g1028-g1036. PMID: 9815033, DOI: 10.1152/ajpgi.1998.275.5.g1028.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBicarbonatesBile Ducts, IntrahepaticBombesinCarrier ProteinsChloride-Bicarbonate AntiportersChloridesFluoresceinsFluorescent DyesHEPESHydrogen-Ion ConcentrationIn Vitro TechniquesMaleModels, BiologicalRatsRats, Sprague-DawleySodiumSodium-Bicarbonate SymportersSodium-Hydrogen ExchangersConceptsHCO-3 secretionBile duct unitsDuct unitsAcid-extruding mechanismsEffect of bombesinRat liverCl-/HCOBombesin-stimulated secretionRegulation of intracellularBiliary secretionNormal rat liverBombesinCholangiocytesSecretionUnderlying mechanismLiverBasalPH regulationCounterregulatoryNeuropeptidesExchange activityActivityMolecular Pathogenesis of Cholestasis
Epstein F, Trauner M, Meier P, Boyer J. Molecular Pathogenesis of Cholestasis. New England Journal Of Medicine 1998, 339: 1217-1227. PMID: 9780343, DOI: 10.1056/nejm199810223391707.Peer-Reviewed Original ResearchMaternal cholestasis does not affect the ontogenic pattern of expression of the Na+/Taurocholate cotransporting polypeptide (ntcp) in the fetal and neonatal rat liver
Arrese M, Trauner M, Ananthanarayanan M, Boyer J, Suchy F. Maternal cholestasis does not affect the ontogenic pattern of expression of the Na+/Taurocholate cotransporting polypeptide (ntcp) in the fetal and neonatal rat liver. Hepatology 1998, 28: 789-795. PMID: 9731574, DOI: 10.1002/hep.510280328.Peer-Reviewed Original ResearchConceptsBile duct ligationWeeks of ageBile acid transporterFetal lifeCommon bile duct ligationOntogenic patternMaternal obstructive cholestasisBile acid excretionMRNA levelsEffect of cholestasisSham-operated animalsSteady-state mRNA levelsFunction of NTCPAcid transportersNeonatal rat liverPostnatal time pointsDays of ageMaternal cholestasisBasolateral bile acid transportersPregnant ratsCholestatic ratsLiver weightObstructive cholestasisDuct ligationMC groupEndotoxin downregulates rat hepatic ntcp gene expression via decreased activity of critical transcription factors.
Trauner M, Arrese M, Lee H, Boyer J, Karpen S. Endotoxin downregulates rat hepatic ntcp gene expression via decreased activity of critical transcription factors. Journal Of Clinical Investigation 1998, 101: 2092-2100. PMID: 9593765, PMCID: PMC508797, DOI: 10.1172/jci1680.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Acids and SaltsCarrier ProteinsCells, CulturedCholestasisDNA-Binding ProteinsDown-RegulationEndotoxinsGene Expression RegulationHepatocyte Nuclear Factor 1Hepatocyte Nuclear Factor 1-alphaHepatocyte Nuclear Factor 1-betaLiverMaleMembrane Transport ProteinsNuclear ProteinsOrganic Anion Transporters, Sodium-DependentPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSepsisSymportersTranscription FactorsConceptsNuclear binding activityNtcp mRNA levelsMRNA levelsHepatobiliary transportersMRNA expressionCritical transcription factorTime pointsSepsis-associated cholestasisNuclear factor-kappaBSodium-dependent uptakeEffector cytokinesSequential time pointsEndotoxin administrationPretreatment levelsBinding activityMaximal decreaseBile acidsFactor-kappaBHepatocyte nuclear factor 1Normal levelsHepatic basolateral membraneNuclear factorMarked reductionCoordinated downregulationEndotoxinCyclic AMP stimulates sorting of the canalicular organic anion transporter (Mrp2/cMoat) to the apical domain in hepatocyte couplets
Roelofsen H, Soroka C, Keppler D, Boyer J. Cyclic AMP stimulates sorting of the canalicular organic anion transporter (Mrp2/cMoat) to the apical domain in hepatocyte couplets. Journal Of Cell Science 1998, 111: 1137-1145. PMID: 9512509, DOI: 10.1242/jcs.111.8.1137.Peer-Reviewed Original ResearchConceptsTransport proteinsApical membraneApical domainTransporter-containing vesiclesMultidrug resistance protein 2Protein 2Cell-cell contactMicrotubule-dependent fashionBasolateral plasma membraneApical proteinsEffect of cAMPVesicle trafficAnion transportersSecretory polarityOrganic anion transportersPlasma membraneCanalicular membraneAnion transport capacityTransport activityApical excretionApical poleCanalicular organic anion transportMultispecific organic anion transporterAmphiphilic organic anionsTransport function
1997
Expression of the rat liver Na+/taurocholate cotransporter is regulated in vivo by retention of biliary constituents but not their depletion
Gartung C, Schuele S, Schlosser S, Boyer J. Expression of the rat liver Na+/taurocholate cotransporter is regulated in vivo by retention of biliary constituents but not their depletion. Hepatology 1997, 25: 284-290. PMID: 9021935, DOI: 10.1002/hep.510250205.Peer-Reviewed Original Research
1996
Down-regulation of expression and function of the rat liver Na+/bile acid cotransporter in extrahepatic cholestasis
Gartung C, Ananthanarayanan M, Rahman M, Schuele S, Nundy S, Soroka C, Stolz A, Suchy F, Boyer J. Down-regulation of expression and function of the rat liver Na+/bile acid cotransporter in extrahepatic cholestasis. Gastroenterology 1996, 110: 199-209. PMID: 8536857, DOI: 10.1053/gast.1996.v110.pm8536857.Peer-Reviewed Original ResearchMeSH Keywords3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)3-Hydroxysteroid DehydrogenasesAnimalsCarrier ProteinsCholestasis, ExtrahepaticCommon Bile DuctDown-RegulationHomeostasisLigationLiverMaleOrganic Anion Transporters, Sodium-DependentProteinsRatsRats, Sprague-DawleyRNA, MessengerSymportersTaurocholic AcidTissue DistributionTranscription, GeneticConceptsEcto-adenosine triphosphataseRegulation of expressionMolecular regulationPosttranscriptional regulationGene transcriptionSteady-state messenger RNA levelsTranscriptional activityAcid transportersProtective feedback mechanismComplementary DNAAcid cotransporterMessenger RNA levelsSodium-dependent taurocholateTranscriptionRNA levelsRegulationBile acid transporterMRNA levelsHepatic bile acid transportersCanalicular localizationTaurocholate uptakeAlpha-hydroxysteroid dehydrogenaseExpressionNtcp proteinTriphosphatase
1995
Expression of sodium-independent organic anion uptake systems of skate liver in Xenopus laevis oocytes
Jacquemin E, Hagenbuch B, Wolkoff A, Meier P, Boyer J. Expression of sodium-independent organic anion uptake systems of skate liver in Xenopus laevis oocytes. American Journal Of Physiology 1995, 268: g18-g23. PMID: 7840202, DOI: 10.1152/ajpgi.1995.268.1.g18.Peer-Reviewed Original ResearchConceptsBSP uptakeSkate liverTaurocholate uptakeXenopus laevis oocytesOrganic anion transport systemInjection of oocytesLaevis oocytesLiverAnion transport systemRat liverSulfobromophthalein uptakeUptake systemOocytesMRNADisulfonic acidUptake activityFunctional expressionLiver mRNAUptakeExpressionSerum albuminLower vertebrates
1994
Characterization of cloned rat liver Na(+)-bile acid cotransporter using peptide and fusion protein antibodies
Ananthanarayanan M, Ng O, Boyer J, Suchy F. Characterization of cloned rat liver Na(+)-bile acid cotransporter using peptide and fusion protein antibodies. American Journal Of Physiology 1994, 267: g637-g643. PMID: 7943329, DOI: 10.1152/ajpgi.1994.267.4.g637.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesBase SequenceBlotting, WesternCarrier ProteinsCloning, MolecularFluorescent Antibody TechniqueHumansImmune SeraImmunoblottingLiverMaleMolecular ProbesMolecular Sequence DataOrganic Anion Transporters, Sodium-DependentPeptide FragmentsRatsRats, Sprague-DawleyRecombinant Fusion ProteinsSymporters